1.A Study of Phototoxicity Using the Candida Albicans Test and the Photohemolysis Test.
Seung Hyun HONG ; Hyun Chul CHOI ; Kee Chan MOON
Korean Journal of Dermatology 1995;33(2):268-274
Background: If phototoxic:ity can be predetermined in vitro, the information will aid in prognosticating whether or not caimpounds have a photosensitizing pczrlial. OBJECTIVE: For the evaluation of the phototoxic potentials of reral drugs, we performed the Candida albicans test and the photohemolysis test. METHODS: The Candida altiicans test is based on growth inhibtion of Candida albicans after application of the drug and ultraviolet light A( UVA ) irradiatior and the photohemolysis test is based on hemolysis of red blood cells caused by irradiation ir the presence of photosensitizing compounds. In the Candida albicans test, clear zones around the drug patches were evaluated, which means positive results for the phototoxic potential of the drugs. In the photohemolysis test, the amounts of hemolysis were evaluated by measuring the relar absorbance at 540nm using a spectrophotometer. RESULTS: In the Candida albicans test, ibuprofen, naldix acid, chlorpromazine and thiodiphenylamine showed positive results, whereas others did not the photohemolysis test, griseofulvin, ibuprofen and nalicdixic acid showed increased amounts hemolysis at UVA and ultraviolet light B(UVB) irracliation, and chlorpromazine, thiodiphenylaiair, chlorothiazide and piroxicam showed increased amounts of hemolysis at UVA irradiation only. CONCLUSION: The results showed that both methods were goodness screening tests for demonstrating the phototoxicity of therapeutic drugs.
Candida albicans*
;
Candida*
;
Chlorothiazide
;
Chlorpromazine
;
Dermatitis, Phototoxic*
;
Erythrocytes
;
Griseofulvin
;
Hemolysis
;
Ibuprofen
;
Mass Screening
;
Piroxicam
;
Ultraviolet Rays
2.Ultrastructural In Situ Hybridization Method for Detection of Oncogenic Viruses.
Keun Hong KEE ; Chul Hee CHOI ; Ho Jong JEON
Korean Journal of Pathology 1996;30(8):687-698
In situ hybridization (ISH) is a standard method for localizing DNA or RNA sequences in tissue or cell preperation. The technique was developed at the electron-microscopic level, and enables the precise subcellular localization. A method was developed for detection of specific viral DNA. We have tested various methods and technique to detect specific viral DNA through ISH at the electron microscopic level. Postembedding method of ultrastructural ISH was developed and successfully applied for the detection of human papillomavirus type 16 in squamous cell carcinoma of the uterine cervix and Epstein-Barr virus in EBV-infected leukemia cell line. The following results are made. The best results were obtained using 0.2% glutaraldehyde and 4% paraformaldehyde fixed tissue or cell block. The labelling was best observed on Unicryl resin and Lowicryl K4M resin sections. Epon sections showed no reactivity. Thin sections of Unicryl resin were more easier than Lowicryl K4M resin. Enzymatic predigestion with proteinase K, pepsin and trypsind gave good results. However, high concentration of these produce poor results due to excessive destruction of the cellular components. Alkali treatment with 0.5N sodium hydroxide produced successful results in denaturation of target DNA. The labelling density of gold particles was independent of incubation time or temperature in hybridization step. The viral DNA labelling was localized mainly within the nucleus, both within and at the edge of electron dense regions, and below the nuclear membrane. And the labelling was seen in the form of a dense, roughly spherical shape. In conclusion, the best results are obtained by the conditions that tissue fixed by 0.2% glutaraldehyde and 4% paraformaldehyde solution, embedded with Unicryl resin, protein denaturation by 0.1ul/ml proteinase K, DNA denaturation by 0.5N sodium hydroxide, and reaction with DNA probe.
Humans
3.The psychological influences of shift work by "symptom checklist-90-revision".
Kee Woon CHOI ; Kyung Hwan CHO ; Myung Ho HONG
Journal of the Korean Academy of Family Medicine 1991;12(7):11-21
No abstract available.
4.The psychological influences of shift work by "symptom checklist-90-revision".
Kee Woon CHOI ; Kyung Hwan CHO ; Myung Ho HONG
Journal of the Korean Academy of Family Medicine 1991;12(7):11-21
No abstract available.
5.Tracheal homografts in the canine.
Hong Shik CHOI ; Eun Chang CHOI ; Kwang Moon KIM ; Nam Hoon CHO ; Kee Hyun PARK
Korean Journal of Otolaryngology - Head and Neck Surgery 1991;34(2):304-315
No abstract available.
Allografts*
6.Liposarcoma of the Breast in Male: Report of a case.
Jong Boum CHOI ; Sung Churl LIM ; Keun Hong KEE ; Ho Jong JEON ; Chae Hong SUH
Korean Journal of Pathology 1992;26(3):293-297
Liposarcoma of the male breast is rare. In English literature, nearly 90 tumors of this type can be found, but the majority of citation is female without detail descriptions of gross or microscopic features. Recently, we experienced a case with pleomorphic liposarcoma of the breast. This 62-year-old male patient presented with tumorous mass of right breast for 3 months ago. He underwent radical mastectomy under the impression of breast cancer. Received specimen was a breast and attached nodular bulging mass. The serial section reveal a relatively defined nodular mass consists of yellowish and glistened bulging tumorous lesion, measuring 5x4.5x5cm. Microscopically, there were multiple pleomorphic giant cells composed of plump esoinophilic or microvesiculated cytoplasm and bizarre nuclei with prominent nucleoli. These cells were positive staining for oil-red O. Ultrastructurally, variable sized numerous fatty vacuoles in the cytoplasm were seen.
Female
;
Male
;
Humans
;
Breast Neoplasms
7.Histopathologic Study of the Endometrium in Primary infertility.
Sung Churl LIM ; Jong Boum CHOI ; Keun Hong KEE ; Ho Jong JEON ; Chae Hong SUH
Korean Journal of Pathology 1991;25(3):196-205
This study was performed to fine out the possible causes of primary infertility in female and to provide some diagnostic and therapeutic modalities. A total of 104 cases of endometrium in primary infertility was obtained from the pathology files of Chosun University Hospital and Kwang-ju Christian Hospital during the period of 5 years and 10 months from January, 1984 to October, 1989. The endometrial biopsies were taken on the first of menstruation and classified according to Noyes, Hertig and Rock's criteria. Histologic findings which were compatible with their normal menstrual cycle (premenstrual of late secretory phase and bleeding phase) were noted in 52 cases (50%). Abnormal 52 cases (50%) revealed deficient secretory phase in 36 case (34.6%), proliferative phase in 5 cases (4.8%), irregular proliferation and chronic nonspecific endometritis in 4 cases (3.8%), respectively, endometrial hyperplasia in 3 cases (2.9%), tuberculous endometritis in 2 cases (1.9%), and asynchronous cycle in 1 case (1%). Among the case of dissociated delay was 21 cases (20.2%) and coordinated delay was 15 cases (14.4%). Therefore, deficient secretory phase with dissociate delay was most common abnormal endometrial finding in cases of female primary infertility. The peak age distribution of the female primary infertility in this study revealed 73 cases (63.8%) in patients between the age of 25 and 29. This finding indicated that there was no significant relationship between the age distributions and the endometrial histopathologic findings.
Female
;
Humans
;
Biopsy
8.A Case of Acute Mixed Lineage Leukemia (ANLL with TII) with neutropenic Enterocolitis.
Hyeon Joo CHOI ; Young Suk KOH ; Young Mee HONG ; Seung Joo LEE ; Kee Suk HONG ; Keum Ja CHOI
Journal of the Korean Pediatric Society 1990;33(9):1281-1287
No abstract available.
Enterocolitis, Neutropenic*
;
Leukemia*
9.Clinical Studies on Total Anomalous Pulmonary Venous Connection.
Young Jin HONG ; Kee Hong KWON ; Hae Il CHEONG ; Jung Yeun CHOI ; Yong Soo YOON ; Chang Yee HONG
Journal of the Korean Pediatric Society 1982;25(11):1120-1130
No abstract available.
10.Pseudolipomatosis of the Gastrointestinal Mucosa: Report of 6 cases with analysis of possible factors involved during endoscopic procedure.
Ghee Young CHOE ; Yong Il KIM ; Kyoo Wan CHOI ; Kee Suk HONG
Korean Journal of Pathology 1992;26(1):10-16
Pseudolipomatosis of the colonic mucosa has been recognized as a lesion featured with aggregations of gaseous spaces in the lamina propria, but its pathogenesis remains still unclear. This paper describes 6 cases of pseudolipomatosis occurring in the mucosa of stomach and large intestine, and the possible factors involved in gastrointestinal endoscopic procedure to produce gaseous entrapment in the lamina propria were analysed. All cases received either gastroscopy or colonoscpy before endoscopic biopsy. Mucosal tissues from both stomach and recto-sigmoid colon revealed multiple aggregations of small air-spaces resembling fatty infiltration in the lamina propria. Rarely were similar infiltrations within the muscularis mucosae and adjacent lymphoid follicles. Evidence for pneumatosis cystoides intestinalis or ulcerative colonic lesion was not associated, although one showed a small gastric ulcer nearby. Repeated inflations and deflations of the stomach or colon during the endoscopic procedure with miner mechanical trauma by instrument to the mucosa seem to contribute to its pathogenesis.