Objective To explore the mechanism of early-onset sub-clinical seizures following acute cerebral infarction. Methods Right middle cerebral artery occlusion (MCAO) rats models were made by threads methods. Sub-clinical seizures were detected with recording apparatus at the same time. Glial glutamate transporter-1 (GLT-1) were detected by immunohistochemical method in rats hippocampus. Results Sub-clinical seizures occurred in 27.4% rats following MCAO. The GLT-1 expressions in CA_1 and CA_3 regions were lower in sub-clinical seizure group[(344.5?35.0)?m~2 and (360.4?13.5)?m~2 respectively] than those in the controls[(447.0?22.8)?m~2 in CA_1 region and (402.3?28.5)?m~2 in CA_3 region]. But there were not significant differences of GLT-1 immunoreactivities in dentate gyrus between the two groups above. Conclusion Early-onset sub-clinical seizures occur following the acute MCAO in rats, which should be related to the decreased expression of GLT-1 in CA_1 and CA_3 of hippocampus.

Objective:To explore protective action of ultramicro-powder of Rhizoma Gastrodiae on nerves in the rat of Cerebral ischemia- reperfusion injury.Methods:The local ischemia-reperfusion injury at model was established by cerebral ischemia for 2 hours and reperfusion for 24 hours.HE staining,2,3,5-triphenytetrazolium(TTC)staining,immunohistochemical methods were respectively used to investigate pathological changes of brain slices,the size of cerebral infarction and nervous cell apoptotic relative proteins Bcl-2,Bax expression after treatment with ultramicro-powder of Rhizoma Gastrodiae.Results:(1)Ultramicro-powder of Rhizoma Gastrodiae could reduce the size of cerebral infarction.When the low,middle and high dose groups were compared with the ischemia group there were significant differences(P

The ultrastructural characteristics of the mouse mammary adenocarcinoma cell line (MA782/5S-8102) have been studied by transmission electron microscope. The results show that the cells were irregular in the shape, with abundant microvilli on cell surface. The nucleus was abnormal in shape with the nuclear membrane sunk or swollen, the nucleus/cytoplasmic ratio (N/C) was high. There were many free ribosomes, abundant mitochondrias, dilated rough endoplasmic reticulum in the cytoplasm. Golgi apparatus, lysosomes and lipid droplets were present. Moreover, many cytoplasmic type-A particles and a few immatural type-B particles at the surface of the cell were observed.

Aim To clone LVA calcium channel (also known as T-type calcium channel) from INS-1 cell line derived from rat pancreatic β cells. Methods RT-PCR, 3′ -RACE and 5′ -RACE were used to clone coding sequence of the whole gene. DNA sequencing and genomic DNA walking were used to identify the primary structure features and exons. Results The cloned gene, named as α 1 G-INS, was composed of 6 864 bp, encoding 2 288 amino acids, which shares 96.3％ identity to α 1G, the neuronal T-type calcium channel. Compared to α 1G, the amino acids in membrane-spanning regions of four transmembrane domains and intracellular loop LI-II of α 1G-INS were highly conserved, but there are three distinct regions. This discrepancy was due to alternative mRNA splicing. Scattering on other regions of the molecule there were 10 single amino acid substitutions, which could be explained as site-mutations of the gene. Conclusion A new isoform,α 1G-INS, of T-type calcium channel is successfully cloned from rat insulin-secreting cell line INS-1, which is significant to further understanding many Ca2＋ -involved biologically essential processes.

Objective To investigate the binding ability of the terminase large subunit of Pseudomonas aeruginosa bacteriophage PaP3 to the cos site. Methods The gene tls was amplified from the genome of bacteriophage PaP3 by PCR and subcloned into pMD18-T vector. Then the gene tls cut down from the vector was inserted into the plasmid pQE31 which could give a 6-His tag at the N-terminal of the expressed protein. The recombinant vector pQE-tls was transformed to E.coli. JM109, after induction with IPTG, the expressed bacteria were resuspended and sonicated, then after centrifugation, the inclusion body was obtained. The inclusion body was dissolved with lysis buffer, then the tagged protein was purified by Ni-NTA affinity chromatography and renatured by dialysis. Finally the DNA-binding ability of the fusion protein rTLS was determined by EMSA. Results The expression plasmid pQE31-tls was successfully constructed, and the target protein yield was up to 30% of the total bacterial proteins. After purification and renaturation, the fusion protein rTLS can partially bind the cos fragment. Conclusion The fusion protein rTLS was successfully expressed, purified and renatured. The rTLS has the specific DNA-binding activity. The present work lays the foundation for the further research of the gene tls.

Objective To determine the effect of percutaneous transforaminal endoscopic surgery for elderly patients with lumbar spinal stenosis .Methods 80 senile patients with lumbar spinal stenosis were collected .According to the treatment methods ,they were divided into observation group and control group .40 patients in the observation group underwent percutaneous transforaminal endoscopic treatment ,40 patients in the control group underwent conventional surgical treatment .The operation time , blood loss , hospitalization time , X -ray exposure time , postoperative visual analogue scale( VAS) score and disability index score were compared between the two groups .Results Compared with the control group,the operative time[(61.7 ±12.4)min vs.(89.3 ±31.2)min],bleeding volume[(22.3 ± 7.2) mL vs.(192.7 ±49.1) mL] and hospitalization time [(5.3 ±1.7) d vs.(11.2 ±3.7) d] of the observation group were significantly less,while the X -ray irradiation time [(17.1 ±3.9) min vs.(10.1 ±3.1) min] was significantly longer.As to VAS incision scores,,postoperative 12h[(4.1 ±1.5) points vs.(8.3 ±2.0) points], postoperative 24h[(2.3 ±1.2)points vs.(7.7 ±2.1)points],postoperative 48h[(1.3 ±0.6)points vs.(5.2 ± 1.6)points]and postoperative 72h[(0.5 ±1.9)points vs.(3.1 ±1.1)points]in the observation group were significantly lower than those in the control group .Compared with the control group ,the ODI scores at 6 months after operation[(11.2 ±3.6)points vs.(17.2 ±4.4)points],12 months after operation[(6.1 ±1.3)points vs.(10.3 ± 2.5)points]and 24 months after operation[(2.9 ±0.8)points vs.(6.4 ±2.1)points]in the observation group were significantly lower,the differences were statistically significant (all P <0.05).Conclusion Minimally invasive treatment of lumbar spinal stenosis with minimally invasive surgery has minimal trauma , less bleeding and shorter hospitalization time.It can significantly improve the symptoms of the incision pain and the functional activities of the limbs,and improve the quality of life of the patients ,and it is worthy of further popularization and application .