0.998. The detection limits were in the range of 16-40 ng/L(S/N=3). The quantitative detection limits were in the range of 45-105 ng/L(S/N=10) with recovery percentages ranging from 87.6% to 106% and the relative standard deviations was lower than 3%. Conclusion The method was proved to be satisfactory in precision,accuracy and sensitivity and applicable to the daily test of estrogens in water.

Objective To observe the effect of neuropeptide Y(NPY) on the levels of serum interleukins(ILs)in guinea pigs with experimental allergic encephalomyelitis(EAE).Methods 30 guinea pigs were divided randomly into normol control group,EAE group and NPY group.The NPY was injected into the intracerebroventricular in NPY group one week before the EAE model be made.The EAE molders were made by injecting the homogenate of rat's spinal cord into the plantar of guinea pigs in groups EAE and NPY.Then,the neurological deficits score was observed everyday,the incidence and the delitescence of EAE were observed too.The levels of serum IL-1?,IL-2,IL-4,IL-6 and IL-8 were detected,and the neuropathological examination was used when symotoms peak period of EAE models.Results(1)The incidences of the EAE in groups EAE and NPY were 100% and 90%,the delitescences of EAE were(10.0?4.8)d and(25.4?12.6)d,the neurological deficits scores at the symotoms peak period were(3.60?0.52) and(1.80?1.14),respectively.There were significantly differences between the two groups(all P

Objective To compare the effects of classic intrafascial supracervical hysterectomy(CISH)and laparoscope-assisted vaginal hysterectomy(LAVH)on structure and function of the pelvic floor in women.Methods From March 2003 to December 2005,totally 35 cases of CISH and 44 cases of LAVH were performed in our hospital.The patients were followed up for 22 to 38 months after the operations.During the period,they received physical examination and life quality questionnaire,by which the degree of pelvic organ prolapse and pelvic floor function were evaluated.Results The incidence of cystocele in CISH group was significantly lower than that in LAVH group [11.4%(4/35)vs 34.1%(15/44),?2=5.481,P=0.019],whereas,no significant difference was found in the incidence of rectocele between the two groups [17.1%(6/35)vs 13.6%(6/44),?2=0.186,P=0.666].Seven(15.9%)patients in the LAVH group developed cervix prolapse,while none of the CISH group had such a situation(P=0.016).Life quality questionnaire showed that 5 patients in CISH group and 16 patients in LAVH group were satisfied with their sexual life [14.3%(5/35)vs 38.1%(16/42),?2=5.456,P=0.019].Conclusion CISH is superior to LAVH in protecting the structure and function of the pelvic floor.

Objective To observe the effect of the combined therapy on headache after craniotomy.Methods 30 patients complained severe headache after craniotomy were divided into the treatment group (n=15, received the combined therapy mainly including nerve block) and control group (n=15, received professional medicine). The visual analogue scale (VAS), admission days after surgery, and medical fees of the patients in two groups were compared.Results The VAS scores of the patients in the treatment group decreased significantly after treatment ( P<0.01), the admission days after surgery significantly shortened ( P<0.01), and the fees of examination, medicine, and ward bed significantly decreased compared with the control group.Conclusion The combined therapy mainly including nerve block is effective on headache after craniotomy.

Adult ; Aged ; Aged, 80 and over ; Biliary Tract Neoplasms ; complications ; Cholangitis ; etiology ; Cholestasis ; etiology ; therapy ; Drainage ; adverse effects ; Female ; Hepatic Encephalopathy ; etiology ; Humans ; Intraoperative Period ; Jaundice, Obstructive ; etiology ; therapy ; Liver Neoplasms ; complications ; Male ; Middle Aged ; Pancreatitis ; etiology ; Stents ; adverse effects

Objective:To assess the ability of axillary reverse mapping (ARM) to identify and preserve the arm lymphatics drainage as well as determine its ability to reduce lymphedema. Methods:A total of 300 breast cancer patients who underwent axillary lymph node dissection (ALND) from June 2009 to May 2011 were enrolled in this study. Methylene blue dye (2 mL to 3 mL) was injected into the ipsilateral upper inner arm along the medial intramuscular groove to map the upper extremity lymphatic drainage system prior to ALND. The blue lymphatic and lymph nodes were identified and preserved during the operation. The change in the arm circumference was the selected method of measurement. The difference in the bilateral upper arm circumference was recorded after 2 months (difference≥2 cm is defined as lymphedema). Results:The ARM was performed successfully in 195 (65%) of 300 patients. The incidence of lymphedema was significantly lower in the successfully mapped patients than in the failing mapping patients, with statistically significant difference during follow-up at 6, 12, 18, and 24 months post operation. Conclusion:The ARM technique can identify and preserve the arm lymphatics drainage and prevent upper extremity lymphedema after breast cancer axillary lymphadenectomy.

Background The pathogenesis of age-related cataract is associated with the apoptosis of lens epithelial cells (LECs) caused by oxidative stress.Previous studies showed that intracellular focal adhesion kinase (FAK) pathway can be activated by H2O2 in vitro,which induced apoptosis of cells.To investigate the effect of oxidative on FAK expression in LECs is one of important studies in the prevention of age-related cataract.Objective This study was to investigate the expression and function of FAK in human LECs treated by H2O2.Methods Human LECs strain (HLECs-B3) were cultured in vitro in the low glucose DMEM with 10％ fetal bovine serum.Different concentrations (0,30,50,70,100,300,500,700,1000 μmol/L) of H2O2 were added into the culture medium for 24 hours.The survival rate of the cells was detected by Cell Counting Kit-8 (CCK-8) assay.Cell morphology as well as the expression and distribution of FAK in the cells were observed by immunofluorescent staining under the laser confocal microscope.Apoptosis was observed by hoechst33258 staining,and Western blot assay was used to quantitatively detect the expression and phosphorylation of FAK.Results The survival rate of the cells was (1.00±0.03) ％,(1.24±0.03)％,(1.36±0.24) ％,(0.93±0.02)％,(1.75±0.19)％,(1.37±0.18) ％,(0.64±0.01)％,(0.59±0.11)％,(0.14±0.05)％ in 0,30,50,70,100,300,500,700,1000 μmol/L H2O2 groups,with a significant difference among them (F =95.30,P =0.00).The survival rates of the cells in the below 300 μmol/L H2O2 groups were significantly higher than those in the 0 μmol/L H2O2 group,and survival rates of the cells in the above 500 μmol/L H2O2 groups were significantly lower than those in the 0 μmol/L H2O2 group(all at P＜0.05).After H2 O2 treatment for 24 hours,HLECs-B3 cells transformed from polygon shape to spindle shape and extended pseudopodiums,meanwhile the green fluorescence for FAK exhibited in the cytoplasm.Cell apoptosis was found in the 1000 μ mol/L H2O2 group.Western blot assay revealed that the expressing levels (grey scale) were significantly different among the various groups (F=28.08,P=0.00),and FAK expressing levels in the below 300 μmol/L H2O2 groups were significantly higher than those of the 0 μmol/L H2O2 group; while the expressing levels in the above 500 μmol/L H2O2 groups were lower than those of the control 0 μmol/L H202 group (all at P＜0.05).After treated by different concentrations of H2O2,the phosphorylation level of intracellular FAK (p-FAK) was significantly higher in 3 hours group than that in 30 minutes group (all at P＜0.05).Conclusions H2 O2 can affect the survival,proliferation and morphology of human LECs by activating the intracellular FAK pathway,indicating that FAK may play roles in the regulation process of cell biological behavior.

Objective To explore the clinical features of subcutaneous fluid collection and post-operative headache after craniotomy and assess the efficacy and side effects of the millimeter wave treatment.MethodsA total of 32 neurosurgical patients with post-operative subcutaneous fluid collection were involved in this study and divided into traditional and millimeter wave treatment groups randomly. Subcutaneous fluid volume after 3 days, time for complete fluid absorption, time of hospital stay, incidence of related infections and severe headache between two groups were assessed.ResultsThe fluid reduction is 93.8% in the millimeter wave treatment group and 76.5% in the traditional treatment group 3 days after treatment (P<0.05); time for complete fluid absorption was also shorter in the millimeter wave treatment group; there is no infection in the millimeter wave treatment group and 3 cases in the traditional treatment group, millimeter wave treatment also reduced the occurrence of severe post-operative headache; there is no treatment-related side-effects in the millimeter treatment group.ConclusionMillimeter wave treatment is an efficacious and safe method for subcutaneous fluid collection after craniotomy, and can reduce the occurrences of related infections and post-operative pain.

Objective To explore the clinical features and the management of incision of scalp pain after craniotomy. Methods 129 patients after craniotomy without postoperative neuralgia were involved. The onset, severity, and characteristics of the pain were recorded within 1 week after the craniotomy. Patients with moderate-severe pain were given oxycodone and acetaminophen (treatment group) or rotundine (control) for 3 d. Results 39.5%（51/129）of patients experienced moderate to severe postoperative pain. After treatment, 76.9% （20/26） in treatment group and 36.0% （9/25） in control were released from the pain (P<0.01). The pain intensity differences and sum of pain intensity differences were more in treatment than in control (P<0.01). Conclusion Incision of scalp pain is common after craniotomy. Oxycodone and acetaminophen, called multimodal analgesia is efficacious and safe for it.

OBJECTIVETo explore the improvement of dendritic cells' (DCs) functions in chronic hepatitis B (CHB) patients by two different drugs plasma, i.e., Shen supplementing and detoxification (SSD) and Pi invigorating and detoxification (PID), thus comparing which method was more effective to activate DCs to improve T lymphocyte functions.

METHODSTotally 30 CHB outpatients were recruited. They were assigned to the immune tolerant group and the immune clearance group, 15 in each group. Totally 60 mL peripheral blood was extracted to isolate and develop mature DCs. Chinese compound containing (Liuwei Ganlu Syrup for SSD) and (Sijun Ganlu Syrup for PID) plasma were added to promote the maturation of DCs on the 7th day. Besides, non-drug plasma was taken as the control. On the ninth day, HBV core 18-27 loaded core peptide and its own T lymphocyte were co-cultivated for 72 h. Then T lymphocytes were collected. The expression levels of CD3, CD28, CD4, and CD8, programmed death-1 (PD-1) were detected using flow cytometry.

RESULTSCompared with non-drug plasma, the expression levels of CD3, CD4, and CD28 could be improved, and the expression levels of CD8 and PD-1 could be reduced by the two methods, showing statistical difference (P < 0.05). Besides, SSD containing plasma showed better effect in improving the molecular CD28 expression rate, and reducing the molecular PD-1 expression rate on the T cell surface, showing statistical difference when compared with that of PID containing plasma (P < 0.05).

CONCLUSIONSIn vitro intervention of DCs by SSD and PID containing plasmas combined co-cultivation of its own T lymphocytes could promote the activation of DCs to improve the function of T cells and the expression of T cell surface molecules. Besides, SSD showed more significant effect on infection immune of HBV patients in the tolerance stage.

Adult ; Cells, Cultured ; Dendritic Cells ; drug effects ; immunology ; Drugs, Chinese Herbal ; pharmacology ; Female ; Hepatitis B, Chronic ; immunology ; Humans ; Male ; Middle Aged ; T-Lymphocytes ; immunology ; Young Adult