Objective To evaluate atrial stunning and the recovery of atrial function after successful cardioversion in the patients with atrial fibrillation by utilizing tissue Doppler imaging(TDI) and M-mode echocardiography in atria-ventricular annulus motion, together with pulsed Doppler echocardiography and apexcardiogram. Methods Thirty-four patients with atrial fibrillation received ultrasound examinations in 1 hour,1 day,1 week and 1 month after successful cardioversion. The results were compared with those of 20 age-matched healthy individuals. From the echocardiography apical 4-chamber views, the atrial mechnical activities in the mitral and tricuspid annulus were recorded using two-dimensional echocardiography guided M-mode and TDI. Furthermore, pulsed Doppler transmitral and transtricuspid inflow velocity and apexcardiogram were also recorded. Atrial stunning means the lack of atrial mechanical function after atrial electrical activity was recovered with successful cardioversion. Results Left and right atrial functions after cardioversion were both inferior to those of the healthy ones,but improved with time,up to 1 week right arial function turned basically to normal and 1 month left one recovered to normal.The incidence of atrial stunning in left and right atrium accounted for (20.6)% and (14.7)% respectively at 1 hour after successful cardioversion, (11.76)% and (14.7)% at 1 day, (6.5)% and (3.2)% at 1 week. Conclusions Both left and right atrial functions are lower than the normal after cardioversion,and atrial stunning happens in both atria.Left and right atria recover to normal respectively in 1 month and 1 week.

OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of benzoic acid and salicylic acid in compound benzoic acid ointment.METHODS:Chromatographic condition included Hypersil ODS column and mobile phase of a mixture of potassium dihydrogen phosphate-methanol(78∶22),the detection wavelength was254nm.RESULTS:The linear ranges of benzoic acid and salicylic acid were0.4～2.0mg/ml(r=0.9999)and0.2～1.0mg/ml(r=0.9999);the average re?coveries were98.74%(RSD=0.55%)and98.67%(RSD=0.59%);the intra-day RSDs were0.56%and0.73%(n=5);the inter-day RSDs were0.75%and0.82%(n=5)respectively.CONCLUSION:This method is simple,accurate and quick,and can be used for the quality control of the preparation.

Objective To evaluate atrial stunning after successful cardioversion in patients with atrial fibrillation by utilizing tissue Doppler imaging(TDI),M-mode echocardiography in left atria-ventricuhr annulus motion,together with pulsed Doppler echocardiography and apexcardiogram.Methods Thirtyeight patients with atrial fibrillation who in 1 hour after successfully underwent electrical or pharmacological cardioversion were involved.The peak motion velocity of mitral annulus during atrial contraction period(Am)and peak motion velocity during early diastolic period(Em) was detected by TDI.The biggest motion argument of mitral annulus during atrial contraction period(DAD)and biggest motion argument during early diastolic period(DED)was detected byM-mode echocardiography.The blood flow velocity of mitral during early diastole(E)and atrial contraction(A) was also measured by pulsed Dopphr echocardiography and pressure wave during atrial systole was recorded by apexcardiogram.The patients were divided into two groups according to whether they had atrial stunning(atrial stunning group and non-atrial stunning group).Results The incidence of atrial stunning occurred in left atrium was 28.9%(11/38)at 1 hour after successful cardioversion.There was no significant difference in cardioversion method between two groups(P>0.05).The patients in atrial stunning group had longer duration of atrial fibrillation and Larger left atrium(P<0.05).A,Am and DAD in the atrial systole period in atrial stunning group was 0.but in non-atrial stunning group was(43±34)cm/s,(6±4)cm/s,(0.27 ±0.18)cm,respectively,and there were significant difference between two groups(P<0.05).Conclusion Atrial stunning can happen in left atrium after successful cardioversion,and atrial stunning is related to the duration of atrial fibrillation and the dimension of left atrium.

Objective To investigate the effects of adenosine postconditioning (AP) on serum IL-10 and TNF-α concentrations following myocardial ischemia-reperfusion(VR)in rats.Methods Twenty-four SD ratsweighing 180-250 g were randomly divided into 4 groups(n=6 each):group I sham operation (group S);group Ⅱ myocardial I/R;group Ⅲ ischemic postconditioning(group IP)and group Ⅳ AP.Myocardial I/R was induced by 30 rain occlusion of anterior descending branch of left coronary artery followed by 120 min reperfnsion.IP was induced by 3 cycles of 30 s myocardial ischemia followed by 30 s reperfusion at the end of ischemia.In AP group adenosine 1.5 mg/kg was infused at 40μg·kg-1·min-1 before the onset of reperfusion.SP,DP and HR were recorded before ischemia (baseline) at 30 min of ischemia and 30 and 120 min of reperfusion.Arterial bloodsarnples were collected at 120 min of repednsion for determination of serum TNF-α and IL-10 concentrations.Theanimals were then killed.Their hearts were removed for microscopic examination.Myocardial infarct size wasmeasured and myocardial MDA content was determined.Results BP and HR were signilicandy decreased duringreperfusion while myocardial infarct size.MDA content and serum concentrations of IL-10 and TNF-α weresignificantly increased in I/R group compared with group S.Ischemic and adenosine postconditioning significantlyattenuated hypotension,reduced infarct size,myocardial MDA content and serum TNF-α concentration and increased serum IL-10 concentration in group AP and IP as compared with I/R group.There was no significant difference in the above changes between group AP and IP. Myocardial injury was ameliorated in group AP and IP as compared with I/R group. Conclusion Adenosine postconditioning can protect myocardium from I/R injury by increasing IL-10 production and inhibiting TNF-a release.

OBJECTIVE: To study clinical characteristics, diagnosis, pathological types and therapy methods of nonsquamous cell neoplasm in subglottis, in order to improve comprehension of this rare disease. METHOD: To analysis 3 patients' clinical data with noosquamous cell neoplasm in subglottis by reviewing related literature, and to generalize the symptoms, differentiation and treatment experience of nonsquamous cell neoplasm in subglottis. RESULT: Among the 3 patients, one's main complain was hoarseness and the other two's main symptom was inspiratory dyspnea. As diagnosis was definite and acute obstruction of airway was relieved, the neoplasms were removed totally. Two patients were undergone operation through oral cavity by using suspended laryngoscope, and one patient was done surgery by neck approach. Paraffin wax result showed that one patient suffered epithelial-myoepithelial carcinoma, who received radiotherapy after surgery. There is no recurrence in all patients by following up more than 1 year. CONCLUSION The occurrence of nonsquamous cell neoplasm in subglottis is relatively low, and the subglottis should be inspected carefully to avoid missed diagnosis. Operation is the preferred method to treat this kind of disease. To choose a suitable operational manner according to size, location of the neoplasm is crucial.
Female ; Glottis ; pathology ; Humans ; Laryngeal Neoplasms ; diagnosis ; pathology ; surgery ; Male ; Middle Aged

The individuality of mangrove ecotope determined the diversity and specificity of symbiotic microorganisms and their metabolites. However, being confined by the knowledge and cultivation means, the field on mangrove symbiotic microorganisms was on starting. In this paper, the progresses on symbiotic microorganisms of mangrove were reviewed, which hint the essentiality and trends of that field.

The molecular characterization of self-antigens expressed by human malignancies that are capable of elicitation of anti-tumor immune responses in patients has been an active field in hematology, oncology, and tumor immunology. More than 2000 tumor antigens have been identified. These significant progresses have led to the renaissance of tumor immunology and studies on novel anti-tumor immunotherapies in lymphomas, other hematologic malignancies and tumors. However, despite of the progress in the identification of these self-tumor antigens, current antigen-specific immunotherapies for tumors are far less satisfactory than that expected, which reflects the urgent need to improve our understanding on the basic principles underlying the selection of these self-tumor antigens. In order to develop more effective antigen-specific anti-tumor immunotherapies and to monitor the responses to these immunotherapies in patients with lymphomas and other malignancies, many additional questions need to be addressed. In this brief review, the progress in the identification of tumor antigens in lymphomas and other malignancies was outlined and the new principles of self-tumor antigens and their significance for future immunotherapies to these malignancies were summarized.
Antigens, Neoplasm ; classification ; immunology ; therapeutic use ; Autoantigens ; classification ; immunology ; Hematologic Neoplasms ; therapy ; Humans ; Immunotherapy ; methods ; trends ; Lymphoma ; immunology ; therapy ; Neoplasms ; immunology ; therapy

Dental Bonding ; methods ; Dentin ; Dentin-Bonding Agents ; chemistry ; Electricity ; Humans

Objective To investigate the characteristics and risk factors of the cerebral artery atherosclerotic stenosis in patients aged 90 years and older. Methods A total of 92 patients,aged 90 years or older,admitted to our hospital from July 2009 to July 2014 were respec-tively enrolled in this study. All participants underwent CTA to assess the presence and severity of atherosclerotic stenosis with intracranial and extracranial cerebral artery. The association between atherosclerotic stenosis and related risk factors were analyzed. Results The preva-lence of cerebral artery atherosclerotic stenosis was high in the cohort. The frequency of stenosis with intracranial artery was higher than the frequency with extracranial artery (86. 2% vs 44. 8%). The most common positions of cerebral artery atherosclerotic stenosis were the middle cerebral artery (43. 1%),the posterior cerebral artery (43. 1%),and extracranial vertebral artery (32. 8%). The mean age,body mass in-dex,systolic and diastolic blood pressure,and fasting blood glucose of stenosis group are significantly different from the non-stenosis group in single factor analysis (P<0. 05). Multivariate Logistic regression analysis showed body mass index was the independent risk factor of cere-bral artery atherosclerotic stenosis(β=1. 326,OR=3. 765,P<0. 05). Conclusion The prevalence of cerebral artery atherosclerotic steno-sis was high in the patients aged 90 years and older. Body mass index was the independent risk factor. The weight control should be pay atten-tion to prevent cerebral artery atherosclerotic stenosis and stroke.

Objective To explore the diagnostic value of conventional ultrasound(US) and contrast enhanced ultrasound(CEUS) in predicting extrathyroidal extension of papillary thyroid cancer(PTC).Methods Eighty-five PTCs in 75 patients were selected for thyroid surgery underwent ultrasound and contrast-enhanced ultrasound.The degrees of contact between PTCs and capsule were observed by US and CEUS respectively(0,0-25％,25％-50％,≥50％),and the diagnostic efficiency in different degree of contact (＞0 ％,≥25 ％,≥50％) as preoperative diagnostic criteria were analyzed.The diagnostic efficiency between US and CEUS in predicting extrathyroidal extension of PTC were compared.Results Of the 85 PTCs,extrathyroidal extension was presented in 57 (67.06％) based on pathologic results.When the degree of contact (＞ 0 ％,＜ 25 ％,25 ％-50 ％,≥ 50 ％) was gradually increased,the incidence of extrathyroidal extension of the thyroid cancer was also gradually risen (P ＜0.001).Comparing the sensitivity,accuracy,odds ratio,and Az value of three groups(＞0％,≥25％,≥50％),it showed that the general diagnostic efficiency between two groups(＞0％,≥25％) was similar by US and CEUS.However,the sensitivity and accuracy of ＞0％ contact with the adjacent capsule were markedly higher than those of the other two groups(P ＜0.001).Selecting ＞0％ contact with the adjacent capsule as preoperative criteria,the Az value of CEUS was markedly higher than that of US (Z =2.208,P =0.027).Conclusions The preoperative imaging feature of more than 0％ contact with the adjacent capsule is more sensitive and accurate degree in predicting extrathyroidal extension of PTC.Compared with US,CEUS may serve as a better useful tool to predict extrathyroidal extension of PTC.