1.Effect of group counseling on job burnout of nurses
Ting-Ting YIN ; Ke-Man YING ; Yue KONG
Chinese Journal of Modern Nursing 2012;18(28):3409-3412
Objective To establish the model of group counseling for nurse and observe the effects on job burnout of the nurses.Methods Totals of 40 nurses with occupation burnout were randomly divided into control group (n =20) and experimental group (n =20).During the operation,eight times group counseling applied in the experimental group and traditional head nurse heart-to-heart talking was applied in the control group.Nurses were tested with nurse job burnout scale (NBS) before and after intervention.Results No significant difference was found in the NBS,pressure source,occupation burnout,personality characteristic,coping style,physiological and psychological symptom scores between two groups before the intervention (P>0.05).The experimental group,as compared to control group,the scores of NBS,pressure source,occupation burnout,personality characteristics,coping style,physiological and psychological symptom were statistically lower after the intervention [(144.15 ±5.37) vs (186.95 ±6.38),(43.95 ±2.52) vs (46.95 ±2.37),(23.05 ±1.70) vs (34.35 ±2.23),(23.35 ±2.06) vs (33.70±1.95),(15.80±1.82) vs (25.05 ±2.11),(38.00 ± 2.10) vs (46.90 ± 5.21) ;t =22.951,3.874,18.016,16.325,14.814,7.084,respectively; P <0.01].The values of heavy workload,non-nursing work to ambiguity,somatic symptoms in two groups had no significant differences (P > 0.05).Conclusions The group counseling mode established by researcher can relieve the job burnout of the nurse.
2.High vaginal uterosacral ligament suspension for treatment of uterine prolapse
Yong-Xian LU ; Wen-Fie SHEN ; Xin LIU ; Jing-Xia LIU ; Ying-Hui ZHANG ; Ying ZHAO ; Lin ZHANG ; Man-Luo HU ; Jing GE ; Ke NIU ;
Chinese Journal of Obstetrics and Gynecology 2000;0(12):-
Objective To evaluate the physiological and anatomic basis,indications,surgical skills, prevention of ureter injury and clinic outcomes of using high uterosacral ligament suspension(HUS)for correction of advanced uterine prolapse by the vaginal route.Methods Fifty women with advanced uterine prolapse underwent transvaginal HUS after vaginal hysterectomy with reconstruction of pubocervical and rectovaginal fascia to correct their uterine prolapse between June 2003 and September 2007.The average age of the women was 60.1 years.The mean follow-up period was 24 months(range 4-51 months).The degree of pelvic organ prolapse preoperatively and anatomic outcomes postoperatively were assessed with pelvic organ prolapse quantification system(POP-Q).Results The remnants of the uterosacral ligaments were clearly identified and palpated posterior and medial to the ischial spines by traction with a 24 cm long Allis clamp and used for successful vaginal vault suspension and reconstruction in all 50 consecutive advanced uterine prolapse patients.The ureter injury was avoided by complete knowledge of the ureter's course from the cervix/apex toward its insertion in the sacral region and how far outside of the uterosacral ligament,by uteri palpation and by suturing purposefully placed"deep"dorsally and posteriorly toward the sacrum,as well as by cystoscopy examination of the spillage of urine from both ureters.Mean POP-Q point C improved from 1.5 to-7.5 cm with a median follow-up of 24 months.If the successful HUS was defined as point C≤stage I prolapse,both the objective and subjective cure rates were as high as 100% with a maximum follow-up of 51 months.None of the 50 patients had repeat operation for recurrence of prolapse.There was no major intra-or postoperative complications,such as ureter and other pelvic organ injury.Conclusion HUS with fascial reconstruction seems to be a safe,minimal traumatic,tolerable and highly successful procedure for vaginal repair of advanced uterine prolapse.Because of the use of native tissue as suspension site HUS is more physiologic and cost effective.
3.Result of serology test for yellow fever virus (YFV) in the entry-exit persons in Guangzhou frontier port and the forecast of risk on YFV infection.
Man-quan LI ; Jin-feng ZHANG ; Jian-ming ZHANG ; Wei KE ; Jing BAI ; Yun-kai YANG ; Rong-rong LIANG ; Ying MENG ; Yan-rong ZHOU ; Mei-li CHEN
Chinese Journal of Epidemiology 2012;33(5):544-545
4.Analysis of lymphocyte immune abnormality in 52 cases of children idiopathic aplastic anemia.
Chun CHEN ; Jing WEI ; Ying-Fei LI ; Shao-Liang HUANG ; Jian-Pei FANG ; Dun-Hua ZHOU ; Hong-Man XUE ; Ke HUANG
Journal of Experimental Hematology 2008;16(5):1091-1095
This study was aimed to investigate the characteristics of lymphocyte immune abnormality in children idiopathic aplastic anemia (IAA) in order to explore the immune pathogenesis of childhood IAA. The phenotypes of lymphocytes, the ratios of Th1/Th2 and Tc1/Tc2, the levels of CD25(+) and CD4(+)CD25(+) T lymphocytes in peripheral blood of IAA patients were measured at the onset of disease by flow cytometry and were compared with that in normal controls. The influences of those immunological indicators on prognosis of IAA were also analyzed. The results showed that there were 40 cases of severe aplastic anemia (SAA) and 12 cases of mild aplastic anemia (MAA). The levels of CD3(+) CD8(+) T cells in SAA group and MAA group were significantly higher than those in controls (p < 0.05). The levels of CD3(+) and CD3(+) CD4(+) T cells in MAA group were lower than that in SAA group (p < 0.05), but there were no difference was compared with control group. No differences of the levels of CD3(-)CD19(+) T cells were between the both SAA and MAA groups and the control group. The levels of CD3(-)CD56(+) T cells in SAA group and MAA groups were lower significantly than that in control group. As compared to control group, the levels of Th1 and Tc1 in SAA group and MAA groups increased significantly (p < 0.05), and the ratios of Th1/Th2 and Tc1/Tc2 in SAA group and MAA groups increased significantly (p < 0.05). The level of Th2 increased in SAA group. As compared to MAA group, the levels of Th1 and Tc1 and the ratios of Th1/Th2 and Tc1/Tc2 in SAA group increased significantly (p < 0.05). The levels of CD25(+) T lymphocyte in SAA group and MAA group increased significantly (p < 0.05), and were higher than that in normal controls, but levels of CD4(+)CD25(+) T lymphocyte and ratio of CD4(+)CD25(+)/CD4(+) in SAA group and MAA group had no significant difference. It is concluded that the abnormal lymphocyte immune function exist in the onset of childhood IAA. The polarization of Th1/Th2 and Tc1/Tc2 shifts to Th1 and Tc1 cells. These changes closely relate to severity of the disease. There is high level of CD25(+) T lymphocyte in children IAA. These changes reveal that abnormality of immune function plays an important role at the onset of childhood idiopathic aplastic anemia.
Adolescent
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Anemia, Aplastic
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immunology
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Child
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Child, Preschool
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Female
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Humans
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Infant
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Lymphocyte Count
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Male
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T-Lymphocytes, Cytotoxic
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immunology
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Th1 Cells
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immunology
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Th2 Cells
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immunology
5.Effect of Tiantai No.1 on gene expression profiles in hippocampus of Alzheimer's disease rats by bioinformatic analysis.
Ying-hong LI ; Zheng-zhi WU ; Mei-qun CAO ; Ming LI ; Ke-huan SUN ; Min YANG ; Man-yin CHEN ; Andrew C J HUANG
Chinese journal of integrative medicine 2015;21(2):123-131
OBJECTIVETo study the effect of Tiantai No. 1 [symbol in text] on gene expression profile in hippocampus of Alzheimer's disease (AD) rat, molecular genetic target points of the effect of this drug were defined, its molecular genetic pharmacodynamic mechanism of anti-AD was further explored at molecular gene level, and a scientific basis was provided for its clinical availability and promotion.
METHODSThirty male Sprague-Dawley rats were divided into three groups with 10 rats per group: sham-operation group, model group and Tiantai No. 1 group. Sterile surgical procedure was applied, the model group with bilateral hippocampal injection of Aβ1-40 was established, and normal saline was used instead of Aβ1-40 in the sham-operation group. One week after the models was made, rats were administered by gastric lavage once every day for three consecutive weeks. The rats of the sham-operation group and the model group were daily fed with purified water by lavage; the rats of the Tiantai No.1 group treated group were administered with Tiantai No.1 by lavage. Total RNAs of hippocampus tissues were extracted with Trizol, the changes of hippocampus gene expression profiles in the above three groups were analyzed by using Affymetrix rat whole genome expression profile microarray.
RESULTSMicroarray analysis showed that, compared with the sham-operation group, the hippocampus of the model group had 50 up-regulated genes with significant difference (fold change >2), and 21 down-regulated genes with significant difference (fold change <0.5); compared with the hippocampus of the model group, the hippocampus of the Tiantai No. 1 group was found to have 5 up-regulated genes with significant difference (fold change >2) and 20 down-regulated genes with significant difference (fold change <0.5). The functions of differentially expressed genes of the groups were involved in nervous system's development, neuronic differentiation and function-regulation, cellular growth and differentiation and apoptosis, synaptic occurrence and plasticity, inflammation and immune response, ion channels/transporters, cellular signal transduction, cellular material/energy metabolism and so on.
CONCLUSIONTiantai No. 1 can regulate hippocampal function, and further regulate the brain function of animals in multiple gene target points by a number of ways.
Alzheimer Disease ; genetics ; pathology ; Animals ; Body Weight ; drug effects ; Computational Biology ; methods ; Drugs, Chinese Herbal ; pharmacology ; Electrophoresis, Agar Gel ; Gene Expression Profiling ; Gene Expression Regulation ; drug effects ; Hippocampus ; drug effects ; metabolism ; pathology ; Male ; Nucleic Acid Denaturation ; Organ Size ; drug effects ; RNA ; isolation & purification ; metabolism ; Rats, Sprague-Dawley
6.Clinical study of 28 patients with adult-to-infant living donor liver transplantation.
Ming-man ZHANG ; Lu-nan YAN ; Cong-lun PU ; Ying-cun LI ; Quan KANG ; Chun-bao GUO ; Xiao-ke DAI ; Zhi-mei REN ; Yu-hua DENG
Chinese Journal of Hepatology 2010;18(10):754-757
OBJECTIVETo summarize our experience in adult-to-infant living donor liver transplantation (A-ILDLT) and to analyze the efficacy and complications of A-ILDLT.
METHODSThe clinical data, surgical strategies and complications of 28 adult donors and infantile recipients who underwent A-ILDLT from April 2006 to December 2009 were retrospectively analyzed. These 28 patients (14 boys and 14 girls) aged from 80 days to 11.5 months with body weights of 3.08 to 10.3 kg at the time of operation . They suffered from biliary atresia with decompensated cirrhosis. The living donors were 15 mothers, 9 fathers, 3 grandma and 1 elder brother with ABO compatible with the infantile recipients. 27 Donor organs were the left lateral lobe grafts (segment II, III) and 1 graft was segment II. All patients were followed up for 5 to 24 months.
RESULTSThese grafts were orthotopically transplanted into the infantile recipients. The average length of stay was 9.3 days for the donor group without any complications. Postoperative immunosuppression included prednisone, Cyclosporin and mycophenolate mofetil (MMF). A total of 24 postoperative complications occurred in 20 recipients, including 5 vascular complications, 4 bleeding, 7 pneumonia, 2 bowel obstruction, 4 intestinal perforation and 3 rejection. Three recipients died of hepatic arterial thrombosis (HAT). The perioperative mortality rate of recipients was 10.7% (3/28) and the survival rate was 89.3% in peroperative period. One died of stricture of hepatic vein and 1 of accidental asphyxia during follow-up term. At present, 23 cases are still alive.
CONCLUSIONA-ILDLT has become an effective method to infants with end-stage liver disease. The postoperative vascular complication is the predominant cause of death.
Female ; Humans ; Infant ; Liver Diseases ; surgery ; Liver Transplantation ; methods ; Living Donors ; Male ; Retrospective Studies ; Treatment Outcome
7.A novel missense mutation, GGC(Arg454) --> TGC(Cys), of CYP11B1 gene identified in a Chinese family with steroid 11beta-hydroxylase deficiency.
Zheng-qin YE ; Man-na ZHANG ; Hui-jie ZHANG ; Jing-jing JIANG ; Xiao-ying LI ; Ke-qin ZHANG
Chinese Medical Journal 2010;123(10):1264-1268
BACKGROUNDSteroid 11beta-hydroxylase deficiency (11beta-OHD), an autosomal recessive inherited disease, accounts for 5% - 8% of congenital adrenal hyperplasia. It was scarcely reported in China. This article reports two Chinese girls with 11beta-OHD.
METHODSThe two patients were sisters and presented with hypertrichosis, skin pigmentation, laryngeal prominence and virilization of external genitalia. The patients were followed up for their clinical symptoms and signs, hormone profile, and adrenal image. The genomic deoxyribonucleic acids of the patients and their parents were isolated. 11beta-hydroxylase gene (CYP11B1) was amplified by polymerase chain reaction and directly sequenced.
RESULTSHormone tests showed that serum cortisol was in the low limit of normal range, whereas the concentrations of adrenocorticotropic hormone, testosterone and progesterone were much higher than those of normal adult females. There were obvious adrenal hyperplasia and advance of bone age. After 11 months of treatment with dexamethasone, the skin pigment became regressed; the breast, uterus and ovary gradually developed and normal menstrual cycle started while the manifestations of virilization did not change. A single point mutation of CYP11B1 (R454C, GGC --> TGC) in all the members of this family was detected. The sisters were homozygous and their parents were heterozygous.
CONCLUSIONSThe clinical manifestation of 11beta-OHD is complicated. The manifestation of virilization could not regress after treatment with dexamethasone. The novel missense mutation of CYP11B1 (R454C, GGC --> TGC) is the pathogenesis of 11beta-OHD at least in some Chinese patients.
Adrenal Hyperplasia, Congenital ; diagnosis ; drug therapy ; genetics ; Child ; Dexamethasone ; therapeutic use ; Female ; Glucocorticoids ; therapeutic use ; Humans ; Male ; Mutation, Missense ; genetics ; Steroid 11-beta-Hydroxylase ; genetics
8.Research progress and therapeutic perspective of iron transport balance based on "iron-inflammation" homeostatic coupling theory
Li-na YANG ; Xin-ke DU ; Li LIU ; Man-jing LI ; Qing-sen RAN ; Qing YANG ; Li-dong SUN ; Yu-jie LI ; Ying CHEN ; Xiao-xin ZHU ; Qi LI
Acta Pharmaceutica Sinica 2022;57(6):1593-1603
The iron and inflammation homeostasis are closely coupled, forming an integrated functional unit under physiological conditions. "Iron transport balance" has become the key mechanism to maintain iron homeostasis through bidirectional regulation of iron uptake and release and dynamic management of transmembrane concentration. It is also the physiological basis for the inflammatory balance between promotion and resolution. Under pathological conditions, represented by inflammatory bowel disease (IBD), disturbed iron transportation was highly involved in almost every step of inflammatory diseases. Therefore, the iron transporting rebalancing provides the mechanistic basis and effective approach for the normalization of inflammatory microenvironment. Macrophage is the key regulator of inflammation homeostasis and determinant for iron transport balance. Unfortunately, the current clinical transformation based on iron transport balance theory has still been insufficient. Sometimes, this strategy even showed high complexity and contradiction, severely restricting its clinical application. By summarizing the theoretical research progress of iron transport balance, especially its relevance to macrophage phenotypic polarization, this review aims to explore the therapeutic value in inflammation intervention by targeting iron transporting balance. This review will provide the necessary knowledge and hints for the research and development of candidate drugs in treating inflammatory diseases.
10.A new indole alkaloid from bulbils of Dioscorea opposite Thunb.
Wei-sheng FENG ; Meng-huan GUO ; Yi-ge YIN ; Yan-gang CAO ; Cui-lan YANG ; Yang-yang WANG ; Man QI ; Yan-li ZHANG ; Ying-jie REN ; Yan-ling LIU ; Xiao-ke ZHENG
Acta Pharmaceutica Sinica 2018;53(7):1131-1133
This study was designed to study the chemical constituents from bulbil of Dioscorea opposite Thunb.. Four compounds were isolated by silica gel column chromatography. On the basis of physic-chemical characters and spectroscopic data analysis, these compounds were identified as lyzalkaloid (3,4-dihydro-6-hydroxy-4-methyl-6H-pyrido[6,5-b]indol-5(1H)-one) (1), anoectochine (2), ginsenine (3), and 2-hydroxy-3-(1H-indol-3-yl) propanoic acid methyl ester (4). Compound 1 is a new indole alkaloid, named as lyzalkaloid. Compounds 2-4 were isolated from this plant for the first time. The cytotoxic activities were assessed by MTT assay. All compounds exhibited the cytotoxic activity against HepG2 and MDA-231 with IC50 values of over 100 μmol·L-1, respectively. All compounds show no significant cytotoxic activities against HepG2, MDA-231 cancer cell.