BACKGROUND:Tetramethylpyrazine (TMP) can inhibit the expression of vascular endothelial growth factor (VEGF), but it is uncertain that TMP inhibit the growth and proliferation of HL-60 leukemic cells induced by VEGF.OBJECTIVE:To observe the effect of TMP on the proliferation of HL-60 leukemic cells induced by VEGF.DESIGN:Repetitive measurement and observation.SETTING:School of Medicine, Wuhan University of Science and Technology.MATERIALS:The experiment was carried out in the Molecular Biology Laboratory Center, School of Medicine, Wuhan University of Science and Technology from March to June in 2007. Human leukemic cell line HL-60 cells were purchased from Shanghai Institute of Cell Biology. TMP hydrochloride injection was produced by Wuxi Seventh Pharmaceutical Products Limited (Lot number:011014), protamine sulfate injection was produced by Shanghai First Biochemical Pharmaceuticals (Batch number:010302), and immunohistochemistry kit was purchased from Boster company.METHODS:①Human leukemic cell line HL-60 cells at log phase were used for the experiments. Cells were treated with 100 μg/L VEGF, and then TMP at final concentrations of 1.5, 15, 150 mg/L was added into culture medium. While the cells in medium without TMP were taken as blank control group, and the cells in medium with 20 mg/L protamine as positive control group. Meanwhile cells without treatment of VEGF were served as VEGF control group. After cells were incubated for 48 hours, the growth inhibiting rate of HL-60 cells was detected by MTT assay.②After HL-60 cells were treated with TMP at the final concentrations of 1.5, 15, 150 mg/L for 24 hours, the protein expression of VEGF in HL-60 cells was examined by SP immunohistochemistry.MAIN OUTCOME MEASURES:①Growth inhibiting rate of HL-60 cells.②Protein expression of VEGF.RESULTS:①Growth inhibiting rate of HL-60 cells:After HL-60 cells induced by VEGF were treated with 15 and 150 mg/L TMP, the absorbance value was significantly lower than that in VEGF control group (P < 0.05).②Protein expression of VEGF:After HL-60 cells were treated with TMP for 24 hours, the protein expression of VEGF was down-regulated with increasing TMP concentration in a dependent manner. Significant differences were observed in the protein expression of VEGF between cells treated by TMP and the controls (P < 0.01).CONCLUSION:TMP can inhibit the proliferation of HL-60 cells stimulated by VEGF and down-regulate the expression of VEGF.

OBJECTIVE: To assess the efficacy and safety of Sanjin tablets in the treatment of urinary tract infection. METHODS: Retrieved from electronic databases and references literatures by on-line or manual way, RCTs about Sanjin tablets in the treatment of urinary tract infection were collected. Related data were obtained and analyzed with evidence-based medical software statistically. RESULTS: 1 046 patients in 13 studies with were included. Two studies showed that Sanjin tablets had better effect than amoxicillin, ciprofloxacin, erythromycin and norfloxacin in the treatment of urinary tract infections. One study showed there was no significant difference in efficacy of Sanjin tablets and Modified juling tang. Other trials showed that there was no difference in efficacy between Sanjin tablets and Chinese herbal medicine, antibiotics. Three trials showed Sanjin tablets combined with Chinese herbal medicine or antibiotics had the same effect with antibiotics alone in the treatment of urinary tract infection. No severe ADR induced by Sanjin tablets was found in included studies. Funnel plot which assumed asymmetry indicated the existence of publication bias and unsatisfactory methodological quality. CONCLUSION: Few trials show efficacy of Sanjin tablets are better than antibiotics. Most of studies show that there is no significant difference between Sanjin tablets and antibiotics. At present no evidence indicate that efficacy of Sanjin tablet is superior to other drugs. The methodological quality of clinical trials of Sanjin tablets for urinary tract infections should be improved.

Objective To assess the efficacy of nebulized budesonide and salbutamol in the treatment of asthma by Meta‐analy‐sis .Methods According to the requirements of Cochrane systematic review ,a through literature search was performed among Wan‐fang database ,CNKI database ,VIP database of Chinese Periodicals and Pubmed database .Meta‐analysis was conducted on a total of 4 649 patients involved in 51 papers that met the inclusion criteria by RevMan4 .3 software .Results Compared with control group (RR=1 .27 ,95% CI=1 .23-1 .32) ,budesonide group which was treated by atomization inhales of nebulized budesonide based on routine treatment(RR=1 .31 ,95% CI=1 .16-1 .49) and salbutamol group which was treated by atomization inhales of salbutamol based on routine treatment(RR=1 .23 ,95% CI= 1 .17 -1 .28) ,the combined group which was treated by atomization inhales of nebulized budesonide and salbutamol based on routine treatment exhibited higher rates of total effectiveness with significant inter‐group difference when taking rates of total effectiveness as index(P<0 .01) .When taking hospitalization rate as index ,the result of the combined group was lower ,compared with the salbutamol group (RR=0 .34 ,95% CI=0 .13-0 .90) ,and the intergroup differ‐ence was significant(P<0 .05) .Conclusion The effect of nebulized budesonide and salbutamol in the treatment of asthma is cer‐tain .

Isokinetic training, as a technique of orthopaedic rehabilitation, is a new and valuable method in evaluation of muscle function and in training muscle strength. It has higher accuracy and reproducibility in evaluation of muscle function. When isokinetic training of muscle strength is done, kinetic velocity is fixed beforehand in the dynamic instrument so that the resistance matches the actual force applied by the individual. The resistance maintains the maximum throughout the range of motion and produces the greatest muscle strength. As a result, the isokinetic muscle strength is effective and safe in the orthopaedic rehabilitation.

Objective:To investigate the efficacy of postoperative chemotherapy in patients with early-stage pulmonary large cell neu-roendocrine carcinoma (LCNEC) after resection. Methods:A cohort of 50 patients who underwent resection and systematic nodal dissection for LCNEC between January 2008 and December 2014 in our institution was retrospectively reviewed. The patients were divided into adjuvant chemotherapy group (32 cases) and non-chemotherapy group (18 cases). Follow-up information was investigated. Results:The median survival and the 5-year survival rate were 48 months and 72.5%for the adjuvant chemotherapy group whereas 29 months and 35.6%for the non-adjuvant chemotherapy group, respectively. Univariate and multivariate analyses using Cox's proportional hazard models showed that postoperative chemotherapy was a signifi cant prognostic factor for OS (P=0.005;hazard ratio=0.281, P=0.008, respectively). Conclusion:Postoperative chemotherapy is beneficial to patients with early-stage pulmonary LCNEC after complete resection.

Objective To assess the efficiency of central urban and rural hospitals in the 7 cities, counties and districts under the jurisdiction of Hangzhou City and the status of the medical markets so as to provide theoretical basis for the promotion of decentralized medical markets. Methods On the basis of defining the scope of a medical market, the HHI values of the medical markets in places where 37 hospitals in Hangzhou were located was calculated, the average efficiency of the various hospitals was measured by means of DEA, and an analysis was made on the correlation between the average efficiency and the HHI values. Results The central urban and rural medical markets in the various places surveyed, whose HHI values were all greater than 1 800, belonged to markets of monopolistic competition. Of the 37 hospitals, there were 11 whose relative efficiency reached 100% and another 11 whose relative efficiency was less than 60%. The greater the monopoly of a medical market, the lower the average efficiency of its hospitals. Conclusion Establishing competitive markets with low market concentration is an effective way of enhancing overall hospital efficiency.

Purpose:Analysing the imaging performance of solitary plasmacytoma of spine,to raise the awareness and the diagnosis accuracy.Materials and Methods: Five cases of spinal solitary plasmacytoma confirmed by clinical pathology were retrospectively analysed,to sum up the imaging performance characteristics.Results: All lesions were single vertebra involved,3 cases in thoracic and 2 cases in lumbar,3 cases occurred vertebral compression fracture.X-ray and CT showed osteolytic destruction,expansive growth and soft tissue mass,MRI showed diversity of signals,and remarkable enhancement after contrast injection.Conclusions: X-ray presents lower diagnostic sensitivity,but contributes to exclude multiple myeloma.CT and MRI can depict the morphological features and extent of tumor regions definitely,CT shows better than MRI on the thickening trabecula and residual bone in the tumor,MRI can provide a lot of information for plasmacytoma,including the diagnosis,identification and staging,which is considered as the best imaging diagnostic methods of plasmacytoma.

Objective To prepare complex material—hydroxyapatite/silicone rubber (HA/SR) used in filling parenchyma and to measure and analyse its mechanical properties, looking for proper proportion of material. Methods The complex material was mixed based on different proportion (the HA/SR mass ratio of 23%, 33.3% and 41%), and its respective properties were tested according to national standard and performed statistical analysis. Results With the increasing proportion of hydroxyapatite in complex material, the rigidity of complex material enhanced gradually, but the mechanical property decreased. Conclusion If the HA/SR mass ratio was 23% to 33.3%, the mechanical properties of complex material are equal to that of pure SR. If the HA/SR mass ratio was over 41%, the mechanical properties of complex material decrease so that the complex material can not be used in filling.

Computer laboratory teaching is the most important method to train the computer operating skills of medical students.Therefore,we have attempted the student-centered open-laboratory teaching model.This teaching model can train the computer operating skills and creative ability of students in medical college.