Objective To investigate the epidemiological features and influential factors of prehypertensionin Yongchuan district of Chongqing .Methods The cluster sampling method was adopted to conduct the survey on randomly selected 3 100 local people (≥18 years old) ,it concluded questionnaire ,physical examination ,blood specimen collection and so on .Results The prevalence of hypertension in Yongchuan district of Chongqing adults was 43 .0% .The prevalence of hypertension in male(49 .5% ) was higher significantly than that of female(35 .0% )(P<0 .05);The prevalence of prehypertension was increased with age up and then de‐clined in older age ,it was achieve highest at 40- <50 years old(50 .7% ) .The average age ,smoking rate ,drinking rate ,BMI ,waist circumference ,TC ,TG ,Purine trione of the prehypertension group was higher than that of the normal group(P<0 .05);the results of Logistic regression indicated that the risk factors of prehypertension weresmale ,higher BMI ,abdominal obesity ,hypercholesterol‐emia ,hypertriglyceridemia ,smoking regularly ,drinking regularly and so on(P<0 .05) ,physical activities was protective factor(P<0 .05) .Conclusion The prevalence of prehypertension in the adults of Yongchuan district of Chongqing is high ,relative intervention on these factors should be tookas soon as possible .

OBJECTIVE To compare the detection rates of Ureaplasma urealyticum(Uu),by 4 kinds rits and find out the best one.METHODS Using four kinds of mycoplasma detection kits,namely are French mycoplasmaIST2,Zhuhai Livzon′s ICS,Dark horse′s IES and Shanghai Its Prachanda,under the same conditions,the mycoplasma of the Clinical specimens for 260 cases were tested.RESULTS The detection rate of mycoplasma(Uu) for 48 hours by four kinds of brands kits about mycoplasma liquid culture was as follows 45.4% for French MycoplasmaIST2,36.2% for Zhuhai Livzon′s ICS,37.7% for Dark horse′s IES and 36.8% for Shanghai Its prachanda.The results of susceptibility test: the reagent of IST2 had.the lowest resistance to josamycin,doxycycline,clarithromycin,ofloxacin and azithromycin,and had the better results than the other three kinds of reagents.Mycoplasma reagents form different companies had significant differences in resistance rates to five kinds of antibiotics.CONCLUSIONS The results suggest that when we cultivate clinical mycoplasma should be careful to select of the mycoplasma liquid culture identification kit.

Objective To investigate the expression of netrin-1 in placenta from patients with pre- eclampsia and the relation to placental angiogenesis.Methods Twenty patients with pre-eclampsia(12 mild cases and 8 severe cases)and 20 normal late pregnant women were investigated.The expression of netrin-1 mRNA and protein was determined with RT-PCR and Western blotting respectively.The placenta vascular density was examined by immunohistochemical F8 staining.Results (1)The values of netrin-1 mRNA in normal group and pre-eclampsia groups were 0.51?0.08 and 0.41?0.06;The values of netrin- 1 protein in normal group and pre-eclampsia groups were 26.4?1.8 and 20.5?1.3(P

Objective To study the image examination of renal injuries and discuss renal explorative indications so as to spare the kidney or nephron as much as possible and improve curative rate of diagnosis and treatment. Methods An analysis was done on 286 cases that included 231 cases with close injury, 54 with open injuries, one with iatrogenic injury and 91 with combined injuries. Of all, 212 cases were examined by B-ultrasonography, 163 by CT and 132 by intravenous urography(IVU) and 6 by digital subtraction angiography(DSA); 202 cases were treated with conservative treatment and 84 with operation. Results The diagnostic positive rates of IVU, B-ultrasonography and CT were 67.4%, 72.2% and 87.7%, respectively. Among the operation cases, 42 cases were treated by renal repair, 12 by partial nephrectomy and 30 by nephrectomy. The operation rate was 29.4% and the nephrectomy rate 35.5%. Interventional treatment of the kidney was carried out in three cases. Conclusions For renal injury cases, the first and most important step is to evaluate the injury condition so as to correctly determine whether an operation exploration is needed. The injury conditions and severity are mainly determined by the image examinations that change according to injury cause, injury type and clinical symptoms. Renal exploration or not, and the operation time exert great influence on renal reservation rate and complication rate.

Objective To evaluate the effects of pretreatment with different doses of phosphocreatine on hepatic ischemia-repeffusion (I/R) injury in rats.Methods.Thirty male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 5 groups (n =6 each):sham operation group (group S),hepatic I/R group (group I/R),and pretreatment with different doses of phosphocreatine groups (groups P1-3).Hepatic I/R was induced by 90 min occlusion of the hepatic artery and portal vein entering the middle and left lobes of the liver followed by 4 h reperfusion in anesthetized rats.Phosphocreatine 50,150 and 450 mg/kg were injected via the tail vein at 60 min before ischemia in groups P1-3,respectively.In groups S and I/R,the equal volume of normal saline was given instead.Blood samples were taken from the abdominal aorta at 4 h of reperfusion for determination of plasma alanine aminotransferase (ALT),aspartate aminotransferase (AST),TNF-α and IL-1β concentrations.The rats were then sacrificed and the livers were removed for determination of myeloperoxidase (MPO) activity (by ELISA),intercellular adhesion molecule-1 (ICAM-1) expression (by immunohistochemistry),and cell apoptosis (by TUNEL) and for microscopic examination (by electron microscopy).Results The MPO activity in liver tissues,plasma ALT and AST activities,TNF-α and IL-1β concentrations and the number of apoptotic cells were significantly higher in groups I/R and P1-3 than in group S,while lower in groups P1-3 than in group I/R (P ＜ 0.05).The parameters mentioned above were decreasedin turn in groups P1-3 (P ＜ 0.05).Conclusion Phosphocreatine pretreatment can attenuate the hepatic I/R injury in rats in a dose-dependent manner and inhibition of the inflammatory responses is involved in the mechanism.

BACKGROUND:Treatment methods for high anal fistula include fistula removal, incision and thread drawing, selective mucosal flap displacement, fibrin glue closure, fistula liagtion between sphincter, most of which show many advantages, including long-term healing, a low success rate, high recurrence rate, and high postoperative complication rate.
OBJECTIVE:To observe the clinical effect of acelular extracelular matrix in the treatment of high anal fistula, and to explore a minimaly invasive treatment for high anal fistula.
METHODS: Totaly 100 cases of high anal fistula were randomly divided into treatment group and control group, 50 cases in each group. Treatment group were treated with alogenic acelular extracelular matrix, and control group were treated with traditional low incision with high thread-drawing. Then, we observed and compared the operation time, bleeding volume, postoperative pain score (visual analog scale score), postoperative pain
duration, anal incontinence severity score (Wexner score), wound healing time, one-stage success rate, cure rate, recurrence rate.
RESULTS AND CONCLUSION: Compared with the control group, the treatment group showed lower scores in the operation time, bleeding volume, wound healing time, visual analog scale score, postoperative pain duration, and anal incontinence severity score (P < 0.05), but higher scores in one-stage success rate and cure rate (P < 0.05). There was no difference in the recurrence rate between the two groups. These findings indicate that the alogenic acelular extracelular matrix for treatment of high anal fistula exhibits smal trauma, quick recovery,
short course of treatment, high cure rate and has no damage to the anal function and appearance.

Purpose　The aim is to study the pharmacokinetic of iv thymopeptide in rabbit.Method　Thymopeptide concentration of rabbit plasma was determined by HPLC and pharmacokinetic parameters were computerized with 3P87 program.Results and Conclusions　The pharmacokinetic parameters of iv thymopeptide in rabbit were:Cmax=(30.06±9.27)μg/ml,t1/2(β)=(29.24±23.78)min, Ke=(0.032 3±0.013 8)min-1 and AUC 0→∞=(205.63±46.48)μg*min/ml.

OBJECTIVE To explore the distribution of 16S rRNA methylase and aminoglycoside modifying enzymes in continuous isolates of Pseudomonas aeruginosa(PAE).METHODS The antibiotics susceptibility was tested by K-B method,the 16S rRNA methylase(rmtB) and aminoglycoside modifying enzymes were detected by polymerase chain reaction(PCR),the positive genes were amplified and sequenced by PCR fluorescence spectrophotometry.RESULTS Among 20 strains of PAE,the 16S rRNA methylase and 5 kinds of aminoglycoside modifying enzymes had been detected.The 5 kinds of aminoglycoside modifying enzymes were aac(3)-Ⅱ,aac(6′)-Ⅰb,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ,respectively.And more,the rmtB in the first and third strains was sequenced,and translated into amino acid,and the translated amino acid sequence was compared with GenBank,suggesting there be different amino acid sequence.This was confirmed as two new subtypes.CONCLUSIONS Resistant to antibiotics PAE in our hospital is mainly related to 16S rRNA methylase and 5 aminoglycoside modifying enzymes,and 2 new subtypes of 16S rRNA methylase are discovered.

OBJECTIVE To explore the distribution of ?-lactamase and drug resistant gene mediated by transposon and integron in continuous isolates of Pseudomonas aeruginosa.METHODS The antibiotics susceptibility was tested by K-B method;the ?-lactamase and the outer membrane protein gene oprD2 were detected by polymerase chain reaction(PCR);the genotype of merA encoding Tn21/Tn501 type trandsposon and qacE?1-suI1 encoding Ⅰ type integron was detected by PCR and the positive genes were amplified and sequenced by PCR fluorescence spectrophotometry.RESULTS The TEM type,OXA-2,OXA-10 and CARB of ?-lactamase genes had been detected in 20 strains of P.aeruginosa,but plasmid-mediated ampC enzyme and metallo-?-lactamase had not been detected,the gene oprD2 encoding porins was not detected.The gene merA was detected in 7 strains(35.0%),and the qacE?-sul1 had been detected in 10 strains(50.0%).The gene OXA-2 in the isolate No.2 was sequenced,and translated into amino acid,and the translated amino acid sequence was compared with GenBank,the results indicated that amino acid sequence of OXA in the isolate No.2 was simlar to that in GenBank,exsited 2 different amino acid sequences,so was confirmed as a new subtype.CONCLUSIONS Resistance to ?-lactamase compounds in P.aeruginosa of our hospital is related to TEM,OXA and CARB genes,and integron and transposon contribute to the drug resistance and multidrug resistance in P.aeruginosa.

OBJECTIVE To explore the distribution of related resistance genes of chloramphenicol and tetracycline in Pseudomonas aeruqinosa isolates.METHODS The antibiotics susceptibility was tested by K-B method,catB cmlA,tetA tetB and smr-2 were detected by polymerase chain reaction(PCR),the gene cmlA was sequenced by PCR fluorescence spectrophotometry.RESULTS The drug-resistant rates of 20 strains of P.aeruginosa against 17 kinds of antibiotics ranged from 10.0% to 100.0%,and multidrug resistant strains were found.The gene of cmlA had been detected in 6 strains of 15 resistant P.aeruginosa isolates,but the genes of tetA,tetB and smr had not been detected.The cml was sequenced,and compared with GenBank,the result showed the gene fragment shared 99.0% homology in nucleotides with the GenBank sequences of cmlA7 and cmlA8.CONCLUSIONS Resistance to antibiotics in P.aeruginosa of our hospital is mainly related to cmlA.The resistance to chloramphenicol and tetracycline was not related to tetA,tetB and smr-2.