Objective: To explore influence of rehabilitation intervention on quality of life in patients with coronary heart disease (CHD). Methods: A total of 108 CHD patients were selected from department of cardiology of our hospital. They were randomly and equally divided into routine treatment group and rehabilitation intervention group (received bedside rehabilitation intervention based on routine treatment). The course of treatment was eight weeks. The medical outcomes 36 item short form health survey (SF-36) was used to assess quality of life before and after treatment of two groups. Results: There were no significant difference in scores of SF-36 at primary evaluation between two groups, P>0.05; when re-evaluation after eight-week treatment, there were no significant difference in all scores of SF-36 in routine treatment group compared with before treatment, P>0.05. Compared with primary evaluation and routine treatment group after treatment, there were significant improvements in all indexes [Physiology: bodily pain (69.89±16.84) vs. (54.62±16.77) vs. (56.63±6.74), role-physical (54.56±35.62) vs. (29.84±39.07) vs. (29.44±37.12), general health (65.74±15.82) vs. (48.49±8.79) vs. (48.28±10.29),Psychology: vitality (62.92±14.31) vs. (37.57±16.65) vs. (38.31±16.32), role-emotional (51.37±18.94) vs. (25.05±14.57) vs. (28.27±12.04), social functioning (74.02±9.35) vs. (47.49±9.87) vs. (51.35±9.29), mental health (72.18±13.26) vs. (55.69±14.05) vs. (58.43±11.36), P<0.01 all] except physical functioning in rehabilitation intervention group after treatment. Conclusion: Rehabilitation intervention can effectively improve quality of life in patients with coronary heart disease.

Childhood obesity is a complex disease that is caused by potygene inheritance and environmental factors.It is the main cause of obesity that energy intake is more than energy consumption.Neuroendocrine regulation participates in the pathogenesis of obesity.Nerve fibers are the basic structure and functional units of the nervous system.The study of the relationship between the distribution of nerve fibers and obesity in children may be a useful exploration for the pathogenesis of obesity.Diffusion tensor imaging (DTI) is a widely used magnetic resonance imaging (MRI) technique.It is a mature technique for observing human white matter fibers.It is an important technique to display the the tacts of the white matter,The diagnosis of the disease and the changes of the central nervous system can be deterined by describing the relationship between the white matter fibers and observing whether the integrity of the white matter is destroyed or not,so as to reflect the structure and function of the brain.But in obese children,the research is lacking.The application of DTI in childhood obesity research is reviewed in this article.

Background Glaucoma is common blinding eye diseases characterized by chronic loss of retinal ganglion cells(RGCs).Currently glaucoma pathogenesis is not completely understood,heat shock protein 27 ( HSP27 )may be associated with the pathogenesis of glaucomatous optic neuropathy. Objective Through the establishment of a rat model of high intraocular pressure,detection of the expression of HSP27 antibody in serum and RGCs apoptosis to investigate the role of HSP27 in RGCs apoptosis. Methods Fifty-one clean Wistar rats were divided into high intraocular pressure group (34 rats)and sham operation group( 17 rats)using a random number table.An animal model of high intraocular pressure was established in the right eye by the application of bipolar underwater electrocoagulation on vein of sclera surface in the experimental group,and rats with conjunctiva incision only without electric coagulation were served as sham operation (control).The intraocular pressure of rats of the both groups including experimental and control rats was measured 1,2,4,6,8 weeks after operation and then the rats were sacrificed.1 ml serum was collected from these rats to determine the concentration of HSP27 antibody.The retinas of the rats were isolated and homogenated for the extraction and analysis of the retinal protein by Western blot.Apoptosis of RGCs were assayed by TUNEL.The use of the experimental animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by Kunming Medical Collegc. Results Intraocular pressure was elevated significantly after modeling and remained a high value during the expcrimental duration,showing a significant difference among the different groups ( F =318.502,P＜0.01 ).However,no significant change in intraocular pressure before and after operation in the sham-operation group ( F =2.076,P ＞ 0.05 ).The serum concentration of HSP27 antibody was increased significantly overtime from 1,2,4,6 to 8 weeks after the surgery applied,with a significant difference among various time points( F =154.221,P＜0.01 ),but no obvious difference was seen in the shamoperation group( F =0.422,P＞0.05 ).Importantly,Apoptosis rate of RGCs in the experimental eyes was significant higher as the time prolong after intraocular elevated(x2=856.12,P＜0.05 ).The positive rate of RGCs apoptosis in the rats with high intraocular pressure group was elevated significantly compared to the rats in the sham operation group(P＜0.05). Conclusions High intraocular pressure induces the expressions of HSP27 in retina and the accumulation of HSP27 antibody in rats serum;the elevated HSP27 is associated with the increased cell death in RGCs.

[Objective]To explore and analyze Professor GUO Weifeng's clinical experience in treating posterior circulation ischemic vertigo. [Methods] By learning with the teacher, collection of relevant information and medical records ,to expound Professor GUO Weifeng 's academic thoughts and clinical experience in posterior circulation ischemic vertigo, from aspects of etiology, pathogenesis, and syndrome differentiation and treatment variation, prescription medication, summarizing the characteristics of his prescriptions and ways of treatment as well as exemplifying them. [Results] Professor GUO Weifeng through many years of clinical practice proposes that the basic pathogenesis of this disease is liver with kidney deficiency and hyperactivity of liver-yang. Because of its more associated with obesity and high blood pressure, high blood lipids, diabetes, always mingle phlegm and stasis. The main treatment method is to nourish the Yin of liver-kidney, pacify liver and extinguish wind. Then, transform phlegm and disperse blood stasis. If pathogenic into collateral for a long time,we need using insect drug for treatment. Clinical treatment should be based on positive and evil of the partial ups and downs, the disease of both, flexible adjustment of medication, it is not limited by present method. [Conclusion] Professor GUO Weifeng 's clinical experience in treating posterior circulation ischemic vertigo is effective and worthy of wide application.

Blood-brain barrier (BBB) injury is an important pathological change of ischemic stroke.Occludin constitutes the major component of the tight junction of BBB, which plays a significant role in maintaining the BBB integrity.Cerebral ischemia initiates occludin degradation through the induction of the activation of inflammatory mediators, reactive oxygen, and matrix metalloproteinases, and causes the changes of tight junction cytoskeletal protein interaction, resulting in BBB integrity destruction and increased permeability.This article mainly elaborates the relationships of occludin with BBB, brain edema and hemorrhagic transformation, as well as the possibility of occludin as an intervention target for ischemic stroke.

Objective To investigate the effect of biofilm cleaning agents and multienzyme cleaning agents in the cleaning of tubular instruments. Methods About 200 pieces of intramedullary nail used in tibial fracture and intramedullary nailing were selected and divided into multienzyme cleaning group and biomembrane cleaning group according to the order of recovery. According to the random number table method, each group had 100 pieces. The biofilm cleaning group was cleaned with biological membrane cleaning agent, and the multienzyme cleaning group was cleaned with multienzyme cleaning agent. The cleaning effect of luminal instruments was observed by means of microscopy, dipstick test and ATP bioluminescence assay. Result The cleaning quality and biofilm removal effect of the biofilm cleaning group were better than those of the multienzyme cleaning group (P<0.05). Conclusion Cleaning the luminal instruments with biomembrane cleaning agent can improve the cleaning quality and prevent the formation of bacterial biofilm.

Hemorrhagic transformation(HT) is the most severe complication after tissue-type plasminogen activator (tPA) thrombolysis. It greatly limits the clinical application of tPA. Early blood-brain barrier ( BBB) damage may be an important mechanism for HT. Maintaining the integrity of BBB can significantly decrease the risk of HT. This article review s the roles, mechanisms, and preventive measure of early BBB damage in HT after tPA thrombolysis.

Objective:To study correlation analysis between Ki-67 and p53 expression in breast cancer tissues and preoperative neoadjuvant chemotherapy (NAC).Methods:From January 2014 to September 2015 in our hospital,73 cases of breast cancer patients treated with NAC were selected as the research object.NAC was administered to all patients for 3 weeks,and then the surgery was performed,and the biopsy specimens were performed before and after chemotherapy.Analysis of the effect of the patients and the expression of p53 and Ki-67.Results:In the 73 patients,CR had 24 cases,accounting for 32.88％,PR had 39 cases,accounting for 53.42％,OR had 63 cases,accounting for 86.30％.The results of pathological grading showed that grade 5 were 13cases,grade 4 in 26 cases,grade 3 in 22 cases,grade 2 in 9 cases,grade 1 in 3 cases.There was no significant difference in the expression of p53 in all patients before and after chemotherapy(P＞0.05).However,the expression of Ki67 was significantly lower than that before chemotherapy,and the difference was statistically significant (P＜0.05).The percentage of p53 positive expression in OR patients after chemotherapy was 34.92％,significantly lower than that of the negative expression of 65.08％,and the positive expression of Ki-67 was 71.43％,significantly higher than that of negative expression of 28.57％,the difference was statistically significant (P＜0.05).According to Pearson analysis,the negative expression of p53 in breast cancer tissues was positively correlated with the efficacy of chemotherapy(r=0.689,P＜0.06);Ki-67 positive expression was positively correlated with the efficacy of chemotherapy (r=0.714,P＜0.05).Conclusion:The expression of p53 and Ki-67 in breast cancer tissues and its effect on NAC were closely related to the preoperative,and it can be used to determine the effect of NAC by monitoring the expression of p53 and Ki-67.

Objective To study the chemical constituents from Vernonia chunii of Hainan Province.Methods The constituents were isolated and purified by repeated column chromatography.Their structures were identified on the basis of physiochemical properties and spectral data.Results Four compounds were isolated and identified as:lupenyl propionate(Ⅰ),lupenyl(Ⅱ),oleanolic acid(Ⅲ),and daucosterol(Ⅳ).Conclusion Compound Ⅰ is a new compound,the others are isolated from this plant for the first time.

Blood Gas Analysis ; Female ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; therapy ; Intensive Care Units, Neonatal ; Male ; Respiration, Artificial