Chronic Disease ; therapy ; Constipation ; diagnosis ; therapy ; Gastrointestinal Transit ; Humans

[Objective]To discuss the failure and countermeasure of Ni-Ti shape memory alloy fixer for treatment of limb fracture.[Method]A retrospective survey was performed in 259 cases with fracture treated by Ni-Ti shape memory alloy fixer.Eight cases of the fixer had been ruptured,risen,loosen after operation.[Result]The failure case of Ni-Ti shape memory alloy fixer for treatment of limb fracture were 8 case in 259 case.There were one case of ulna and two cases of femur fracture had be ruptured after the first operation,healing up after the second operation.The fixer has been risen of shoulder bone and femur fracture in each one and two cases of clavicle,the fracture has be malformation concrescence.One case of patella fracture was loosn after concrescence.[Conclusion]The requisite for Ni-Ti shape memory alloy fixer is needed for treatment of limb fracture,the right operation's condition,the best type and length are selected for different part,the convalescent exercise is regnired after operation to avoid failure of treatment.

[Objective]To compare the reliability and value of clinical signs,MRI imaging and arthroscopy in management of meniscal tears in order to increase the diagnostic rate.[Method]A retrospective study was done on 176 cases undergoing MRI examination and arthroscopic treatment,and being diagnosed as meniscal injuries in knee joint by any one of the three clinical,MRI or arthroscopic examinations.Using arthroscopic diagnosis as the standard,the sensitivity,specificity.and overall accuracy of clinical diagnosis and MRI were then calculated respectively.Significant differences between arthroscopic diagnosis and clinical diagnosis or MRI diagnosis were analyzed using statistical method of Chi-square test.[Result]The sensitivity,specificity and accuracy of clinical diagnosis were 79.3%,26.3% and 73.8% respectively.There was statistically significant difference between arthroscopic diagnosis and clinical diagnosis.The sensitivity,specificity and accuracy of MRI diagnosis were 94.1%,92.9% and 93.4% respectively.The difference between arthroscopic diagnosis and MRI diagnosis had no statistically significance and can be used as an important examination before knee arthroscopy.[Conclusion]MRI is a reliable,noninvasive diagnostic tool for meniscal tears and can be used as an important examination before knee arthros copy with MRI examination combining with the clinical signs,diagnotic rate of meniscal tears can be increased.Unnecessary arthroscopic therapy could be avoided.

Corneal neovascularization is a significant and sight-threatening complication of many ocular surface disorders, and may cause corneal sear and rejection reaction after corneal grafting. Recent studies have revealed that vascular endothelial growth factor (VEGF) plays an important role in corneal neovascularization, and inhibition of VEGF has become a main strategy for treatment of corneal neovascularization. This article reviews the research progress in inhibition of corneal neovascularization by anti-VEGF therapy.

Objective To investigate expression of glycogen synthase kinase(GSK)-3? and phosphorylation GSK-3? (p-GSK-3?) in the anterior temporal neocortex in patients with refractory epilepsy(RE). Methods Expression of GSK-3? and p-GSK-3? were detected by RT-PCR, FQ-PCR, Western Blot and immunohistochemistry in the anterior temporal cortices of 36 RE cases. 8 patients without RE had been used as the controls. Results Compared with control group,the expression of GSK-3? mRNA and protein were significant higher(allP

Objective To study the feasibility of transumbilical single-incision laparoscopic surgery ( SILS) in the treatment of tubal pregnancy, as compared with conventional multi-port laparoscopic surgery. Methods Sixty-two patients diagnosed as tubal pregnancy undergoing laparoscopic surgery at this hospital between September 2013 and June 2014 were selected for investigation. There were 29 cases of transumbilical SILS ( SILS group) and 33 cases of conventional multi-port laparoscopic surgery ( MPLS group) . We retrospectively reviewed the medical records of all patients and analyzed the surgical outcomes, including operative time, blood loss, surgical complications and hospital stay. Results In both groups, all procedures were performed without failure.No conversion to open surgery or additional skin incision was needed.There were no differences between the SILS and MPLS groups in the operation time [(51.5 ±10.8) min vs.(47.3 ±9.4) min, t=1.637, P=0.107], the mean estimated blood loss [(15.5 ±10.5) ml vs.(18.4 ±12.2) ml, t=-0.996, P=0.323], the absolute decrease of hemoglobin from preoperative to postoperative [(14 ± 5) g/L vs.(13 ±4) g/L, t=0.874, P=0.386], the postoperative hospital stay [(3.5 ±0.9) d vs.(3.8 ±0.8) d, t=-1.390, P=0.170], and the incidence of postoperative fever (2 cases vs.3 cases, χ2 =0.000, P=1.000).Follow-up for 1-3 months ( mean, 1.8 months) in all the cases showed smooth recovery and no postoperative complications. Conclusion SILS is a feasible and safe approach in the treatment of tubal pregnancy.

Objective:To explore the therapeutic potential of preemptive targeting of the epitope spreading cascade by adoptive transfer after EAE onset of immunoregulatory T cells specific for the MBP87-99 spreading determinant.Methods:EAE was induced by PLP139-151 in SWXJ mice, all mice were weighed and examined daily for neurologic signs. On days 6 after EAE onset, mice were injected with IL-2prom→IL-10cDNA-transfected Th2/Tr1-like T cells specific for MBP87-99,PLP104-117,or BSA. Finally demyelination was quantified by analysis of digitized images of PLP immunostained spinal cord sections.Results:Mice received Th2/Tr1-like T cells specific for MBP87-99 had a significantly improved clinical outcome(P=0.02) compared with control mice, reduction in the relapse rate and delay in mean time to onset of first relapse were also observed(P

Animals ; Chromatography, Gas ; methods ; Hexanones ; blood ; Mice