Objective:To study difference in arterial oxygen(PO 2) pressure between the carotid artery and the femoral artery and its relation with hypoxia of brain after head injury.Methods:Forty patients with acute head injury were divided into four groups according to the GCS(Ⅰ:6 patients for GCS 3-5score;Ⅱ:16 patients for GCS 6-8 score;Ⅲ:8 patients for GCS 9-12 score;Ⅳ:10 patients for GCS 13-15score).The PO 2 of the carotid artery and the femoral artery was monitored for every patient.At the same time,26 patients from all groups were monitored using transcranial dopplergraphy.Additional,22 patients with severe head injury were continuously monitored on PO 2 of the carotid artery and the femoral artery for 5 days.Results:(1)The difference in PO 2 between the carotid artery and the femoral artery was identified in Ⅰ and Ⅱ groups.The PO 2 of carotid artery is much less than that of femoral artery(P

The study analyzes the major factors which h ave led to the differences between the current situation of drug research and de velopment in China and that in the developed countries: Long-time-existing pla nned economy has prevented Chinese medical enterprises from rapidly becoming the principal part in drug research and development; there is a lack of the entrepr eneurs who can establish the drug research and development systems and availa ble market environment; scientists and entrepreneurs have difficulty in cooperat ing well; the reorientation about the government functions leaves much to be desired Based on above factors, this study has developed related measureme nt s to reduce the differences, such as conducting the structural innovation; esta blishing the drug research and developments systems unalterably; rearranging the functions of the governments and optimizing the market environment; strengthen ing the cooperation among the excellent pharmceutical enterprises in China

The incidence of allergic diseases (asthma, allergic rhinitis, atopic dermatitis and food allergy) increased gradually over recent decades, and an increasing number of patients are suffering from allergic diseases, thus the rise in allergic disease incidence warrants concern. Researchers have already had a deeper understanding of the pathogenesis of allergic diseases. Mounting evidence has shown that the eosinophils play an important role in the pathogenesis of allergic diseases. Many systematic studies have been conducted about eosinophils, therefore, this review will provide a summary on recent progress in eosinophils and allergic diseases.
Asthma ; etiology ; immunology ; Dermatitis, Atopic ; etiology ; immunology ; Eczema ; etiology ; immunology ; Eosinophils ; Food Hypersensitivity ; etiology ; immunology ; Humans ; Rhinitis, Allergic ; etiology ; immunology

AIM: To investigate the effects of microRNA (miRNA)-483-3p on the growth and migration of human glioma cell line A172 and its potential mechanisms.METHODS: The abundance of miRNA-483-3p in human embryonic kidney 293 cells and different human glioma cell lines (A172, U251 and SHG44) was measured by RT-qPCR.After down-regulation of miRNA-483-3p by transfection of inhibitor in the A172 cells, the cell viability, cell cycle distribution and cell migration were detected by CCK-8 assay, flow cytometry and Transwell assay, respectively.Furthermore, the protein levels of cell cycle-related molecules and epithelial-mesenchymal transition markers were measured by Western blot.Luciferase reporter assay was used to predict and verify the target gene of miRNA-483-3p.RESULTS: miRNA-483-3p was highly expressed in human glioma cells.Knockdown of miRNA-483-3p inhibited A172 cell viability, arrested cell cycle and decreased cell migration rate.Furthermore, the protein levels of cyclin D1, cyclin-dependent kinase 4, phoshorylated retinoblastoma protein, N-cadherin and vimentin were significantly decreased after knockdown of miRNA-483-3p, accompanied with the up-regulation of E-cadherin and β-catenin protein expression.Luciferase reporter assay demonstrated that Smad4 was a potential target gene of miRNA-483-3p.Down-regulation of Smad4 in the A172 cells transfected with miRNA-483-3p inhibitor partially reversed the effect of miRNA-483-3p on cell viability and migration.CONCLUSION: Knockdown of miRNA-483-3p restrains the growth and migration of A172 cells by targeting Smad4.

Mucosa-associated lymphoid tissue (MALT) lymphoma often occurs in the stomach as well as the head and neck.This disease has characteristics of multi-site dissemination and indolent development,and about 80% of MALT lymphoma patients are in early stage.Helicobacter pylori (Hp) eradication is the main method for treating early-stage Hp-positive gastric MALT lymphoma.For patients with other types of early-stage MALT lymphoma and those with gastric MALT lymphoma who failed Hp eradication therapy,radiotherapy has a good clinical effect.In recent years,radiotherapy target volume has changed from the involved field to the involved site,and the dose has been decreased.This article provides a review of the above aspects.

Objective To explore the causes of occupational exposure and methods of personal protection from occupational exposure.Methods Twenty seven cases with occupational exposure to HIV hospitalized in our hospital from 2003 to 2008 were analyzed retrospectively.Results Of the 27 cases with occupational exposure to HIV,8 were policemen,4-physicians,12-nurses and 3-laboratory technicians.Of them 4(3 medical workers)were of skin exposure,17(16 medical workers)were injured by sharp devices,and 6(all policemen)were injured by scratch or bite.Post exposure prophylactic interventions were provided for all of them,and no infection was identified after 12 month medical observation.Conclusion Regular training of occupational exposure to HIV for medical workers and judicial staff will be needed.

Objective:To investigate the effects of sodium phenylbutyrate(NaPB) on the matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1.Methods: CGTHW-1 cells were treated with different concentrations of NaPB,then the invasive ability of CGTHW-1 cells was assessed using Transwell assay.The expression of MMP-9 and TIMP-1 was examined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells.Results: After treatment with NaPB(4 mmol/L) for 72 h,CGTHW-1 cells passing the Transwell were significantly reduced[(29.8?1.77) vs(11.00?2.59),P

Background and purpose:Gastric cancer is one of the common malignancies seen in our country, most patients were diagnosed at a late stage. Surgery remains as one of the main treatments for the patients but the local local and regional failures were still high. The importance of the role of chemotherapy and radiotherapy has been investigated in the treatment of gastric cancer.We evaluated the efficacy and side-effects of CPT-11 combined with capecitabine for the treatment of advanced gastric cancer. Methods:Since May 2004 to May 2006, twenty patients with pathologically proved gastric cancer have been treated by CPT-11 plus capecitabine in our hospital. The regimen consisted of CPT-11 120 mg/m2 infusion at 1st day and 8th day, capecitabine 1000 mg/(m2?d) twice a day from 1st-14th day . The cycles were repeated every three weeks. All the patients were planned to receive at least 2 cycles of chemotherapy.Results:In 20 evaluable patients, 1 case had complete response; 8 case had partial response; 6 case had stable disease and 5 case with progressive disease. The response rate of the whole group was 45.0%(9/20); Clinical tumor control rate was 75.0%(15/20). The major toxicities were bone marrow suppression (52.1%) and reactions relative to digestive system (35.2%). All of complications were RTOGⅠ/Ⅱ degree.Conclusions:Combination of CPT-11 and capecitabine is effective for advanced gastric cancer and well tolerated by the patients. For the patients with either old age, poor general status or relapse after other chemotherapeutic regimen, the regimen could be the one of choice.

Adolescent scoliosis is spinal deformity happening in early adolescent or before bone mature.It can affect the body figure and appearance of children,threat the physical and mental health of the youth.Serious malformation even leads to heart and lung function failure.This paper summarizes the causes of disease incidence,the incidence situation in domestic and abroad,census method,the latest gene chip technique,the latest progress of treatment,and investigates the method of early prevention,early diagnosis,and early treatment.For younger children,non-surgical treatment such as brace could be used to avoid scoliosis aggravating.

Objective To evaluate atrial stunning and the recovery of atrial function after successful cardioversion in the patients with atrial fibrillation by utilizing tissue Doppler imaging(TDI) and M-mode echocardiography in atria-ventricular annulus motion, together with pulsed Doppler echocardiography and apexcardiogram. Methods Thirty-four patients with atrial fibrillation received ultrasound examinations in 1 hour,1 day,1 week and 1 month after successful cardioversion. The results were compared with those of 20 age-matched healthy individuals. From the echocardiography apical 4-chamber views, the atrial mechnical activities in the mitral and tricuspid annulus were recorded using two-dimensional echocardiography guided M-mode and TDI. Furthermore, pulsed Doppler transmitral and transtricuspid inflow velocity and apexcardiogram were also recorded. Atrial stunning means the lack of atrial mechanical function after atrial electrical activity was recovered with successful cardioversion. Results Left and right atrial functions after cardioversion were both inferior to those of the healthy ones,but improved with time,up to 1 week right arial function turned basically to normal and 1 month left one recovered to normal.The incidence of atrial stunning in left and right atrium accounted for (20.6)% and (14.7)% respectively at 1 hour after successful cardioversion, (11.76)% and (14.7)% at 1 day, (6.5)% and (3.2)% at 1 week. Conclusions Both left and right atrial functions are lower than the normal after cardioversion,and atrial stunning happens in both atria.Left and right atria recover to normal respectively in 1 month and 1 week.