BACKGROUND: Cervical vertebra corpectomy decompression is one of the commonly used methods of anterior cervical decompression. In recent years, under the guidance of the principle of anterior cervical surgery, scholars propose the anterior cervical vertebra corpectomy decompression with preserving the posterior wall of the corpectomied vertebral body. It retains the posterior wall of the vertebral body, not only increases the stability of the cervical spine, but also increases the area of bone graft, which is conducive to the long-term fusion. At the same time, retaining posterior wall of the vertebral body can effectively prevent bone- and implant-induced spinal cord injury.OBJECTIVE: To evaluate the biomechanical stability of anterior cervical vertebra corpectomy decompression with preserving the posterior wall of the corpectomied vertebral body procedure on sheep by the establishment of finite element model with CT data.METHODS: Cervical vertebra specimens of adult sheep were selected, and scanned to capture its CT data. The geometrical model captured by CT was then transformed into finite element model through finite element software (without surgery). In the posterior wall preserving group, C4 corpectomy decompression was conducted while preserving its posterior wall followed by titanium-meshes and AO plate fixation. On the basis of posterior wall preserving group,corpectomy decompression was operated without preserving the posterior wall in another group; titanium mesh and plate were fixed. Finite element software was used to test and analyze the changes in stress and displacement of cervical vertebra under different conditions.RESULTS AND CONCLUSION: (1) The displacement was slightly small, and immediate postoperative stability was good in the posterior wall preserving group. However, the displacement and stress were not significantly different between posterior wall preserving group and posterior wall non-preserving group. (2) To sum up, a procedure preserving posterior wall of the vertebra body gains better immediate postoperative stability compared with conventional method.

Objective To study the effect of Yinxingdamo (compound of ginkgo biloba extract) on hemodynamics of cerebral circulation in patients with acute cerebral infarction (ACI). Methods Sixty patients with first ACI of carotid artery system were randomly divided into a treatment group (30 cases) and a control group (30 cases), and treated with the drug Yinxingdamo plus therapeutic exercise and therapeutic exercise, respectively. The cerebrovascular dynamics indexes (CVDI) of both groups were investigated before and after the treatment. Results The Vmin and Qmin of carotid artery in the two groups were increased after treatment and the cerebrovascular resistance (R) was decreased (P

AIM: To study the effect of sphingosine 1-phosphate (S1P) on the increase in microvessel permeability induced by platelet activating factor (PAF). METHODS: The microvessel permeability was assessed by measuring hydraulic conductivity (Lp). To observe the effect of S1P and PAF on vascular endothelial-cadherin (VE-Cadherin), the microvessels were stained with immunofluorescence and examined by laser confocal microscopy. RESULTS: After giving PAF at concentration of 10 nmol/L, the Lp value of rat mesentery microvessel was significantly increased. However, after pretreatment with S1P, PAF did not give rise to a further significant change. The effect of PAF on microvascular endothelial cells could be seen: the formation of endothelial gap was induced, the microvascular fluorescence intensity significantly increased, a large number of fluorescent microspheres (FMs) distributed in the space among the endothelial cells. However, after pretreated with S1P, no obvious gap opening and the FMs accumulation were observed. Compared to normal control, no significant difference of the microvascular fluorescence intensity was found. CONCLUSION: PAF changes the structure of VE-Cadherin, leading to detachment of adherent junction, formation of intercellular gaps, which contributes to the increase in the permeability. S1P improves the increase in the microvessel permeability caused by PAF, which might be mediated by strengthening adherent junction and inhibiting the formation of endothelial gaps.

Objective To investigate the effect ot ionizing radiation (IR) on the expressions of HMGB1 in the radiation-sensitive and radiation-resistant human cervical cancer cells and to analysis the role of HMGB1 in the regulation of radiosensitivity.Methods Human cervical cancer cells HeLa and its radioresistant strain HeLaR cells were irradiated with different doses of X-rays.The cells were collected at different time points after irradiation.The expressions of protein and mRNA of HMGB1 were detected by Western blot and real-time quantitative PCR.Results At the protein level,the expression of HMGB1 in HeLaR cells was significantly reduced at 6-36 h after 2,5 and 10 Gy X-ray irradiation (t =3.574-9.754,P ＜0.05),and then it was recovered to the control level at 48 h after IR.On the contrary,the expression of HMGB1 in HeLa cells was significantly increased at 6,12,48 h after 2 Gy IR (t =3.945-4.864,P＜0.05),at 6,36,48hafter5 GyIR (t=-2.875-3.295,P＜0.05),and at 36,48 h after 10 Gy IR (t =-4.480,-4.517,P ＜ 0.05).At mRNA level,the trend of HMGB1 expression alteration was consistent with that of protein expression.Conclusions The changes of HMGB1 expression can be differently induced by X-rays in the human cervical cancer radiation-sensitivity cells and radiation-resistant cells.HMGB1 may be involved in the radioresistance of human cervical cancer.

Liposome is an artificially prepared spherical vesicle that has a phospholipid bilayer. Given that the basic structure of its biological membrane is also a lipid bilayer membrane, liposome shares similar structures with body cells Therefore, liposome has good biocompatibility and advantages such as biodegradability, low immunogenicity, and subtle toxicity. Liposome has been widely ap-plied as an effective drug carrier. Studies on liposome-encapsulated fluorescent dye on tumor tracing have been reported in recent years. Liposome can become a more advantageous transport carrier with continuous development of surface modification materials and prepa-ration methods. The long cycle, targeted liposome-encapsulated drugs, and fluorescent dye have become the focus of interest for several researchers. This article mainly discusses the application and progress of long cycle and targeted liposome in cancer research.

Objective To explore the application of the exercise test (ET) in paroxysmal kinesigenic dyskinesia (PKD).Methods We conducted the ET in 33 controls and 45 PKD patients following standardized protocols,and the decrement in the amplitude and in the area of compound muscle action potentials (CMAP) after long ET and other seven parameters were calculated and compared in both groups,and the normal range of parameters was defined as the mean ± two standard deviation of the control values.Results According to the control group,the normal range of the change in the amplitude of CMAP immediately after short ET was-13％-27％ and the normal range of decrement in the amplitude of CMAP after long ET was less than 33％.And the ET was abnormal in 16 of the 45 PKD patients (36％).Comparing the nine parameters between two groups,we found that both the decreases of the amplitude (25.5％ ±13.4％ vs16.2％ ±8.6％,t=-3.72,P=0.00) and the area(31.8％ ±16.3％ vs19.0％ ± 16.8％,t =-2.39,P =0.02) of CMAP after long ET in PKD patients were larger than those in controls.No statistically significant difference was found between 19 non-ion channel disease patients and 14 normal controls.No statistically significant difference was found between the nine parameters in 27 PKD patients before and after medication,though all the patients had obvious clinical improvement.Conclusion We found that abnormal ET may appear in PKD patients,and this finding hinted that abnormal muscle membrane excitability might be an underlying mechanism responsible for PKD.

Objective: To investigate the effect of over-expression of transducer of ErbB2, 1 (TOB1) on migration and invasion of human lung adenocarcinoma cell line A549. Methods: The expression levels of TOB1, phosphatase and tensin homolog deleted on chromosome ten (PTEN), breast cancer metastasis suppressor 1 (BRMS1), ras homolog gene family, member C (RHOC) and non-metastatic cells 1 (NME1) mRNAs in human bronchial epithelial (HBE) cells and ten types of human lung cancer cells were detected by RT-PCR. The recombinant plasmid pcDNA3.0-TOB1 and the empty vector pcDNA3.0 were transfected into A549 cells, respectively. The A549 cells over-expressing TOB1 protein (A549/TOB1 cells) and the A549/PC3 cells (as the control cells) were established. The abilities of migration and invasion of A549/TOB1 cells were determined by wound healing and Transwell assay. The expression levels of PTEN, BRMS1, RHOC and NME1 mRNAs were detected by RT-PCR, and the expression levels of α-N-catenin, β-catenin, γ-catenin, matrix metalloproteinase 2 (MMP2) and MMP9 were detected by Western blotting. Results: The expression levels of TOB1 mRNA in ten types of lung cancer cells were lower than that in HBE cells (P <0.05). The expression level of TOB1 mRNA was positively correlated with the expression levels of PTEN, BRMS1 and NME1 mRNAs (P <0.05). The abilities of migration and invasion of the A549/TOB1 cells were obviously decreased as compared with those of the A549 cells (P <0.05). The expression levels of PTEN, BRMS1 and NME1 mRNAs were higher whereas the expression level of RHOC mRNA was lower in the A549/TOB1 cells as compared with those in the A549 cells (P <0.05). The expression levels of α-N-catenin, β-catenin, γ-catenin, MMP2 and MMP9 proteins in the A549/TOB1 cells were lower than those in the A549 cells (P <0.05). Conclusion: Exogenous TOB1 over-expression can inhibit the abilities of migration and invasion of A549 human lung cancer cells in vitro . This effect may be associated with the downregulation of α-N-catenin, β-cateninm γ-catenin, MMP2 and MMP9 expressions, and which was induced through the regulation of PTEN, BRMS1, RHOC and NME1 expressions by TOB1. Copyright © 2012 by TUMOR.

Objective To study the chemical constituents of sesquiterpenoids of agarwood from Aquilaria crassna. Methods Seven squiterpenoids were isolated and purified by various column chromatographic techniques and HPLC method. The structures of the compounds were identified through the combined analysis of physicochemical properties and spectral data. The acetylcholinesterase and α-glucosidase inhibitory activity of compounds were evaluated by Ellman colorimetric method and pNPG method, respectively. Results Seven compounds were isolated from the ethyl acetate extract obtained from 95% aq. ethanol extract of agarwood from Aquilaria crassna and were identified as 2-[(2β,8β,8aα)-8,8a-dimethyl-1,2,3,4,6,7,8,8a-octahydro naphthalen-2-yl]-3- hydroxy-2-methoxpropanoic acid (1), 2-[(2β,8α,8aα)-8,8a-dimethyl-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl] propane-1,2-diol (2), (1β,3α,4aβ,5β,8aα)-4,4a-dimethyl-6 (prop-1-en-2-yl) octahydronaphtha-lene-1,8a(1H)-diol (3), eremophila-9-en-8β,11-diol (4), eudesma-4-en-8,11-diol (5), eudesma-4-en-11,15-diol (6), and methyl-15-oxo-eudesmane-4,11 (13)-dien-12-oate (7). Moreover, compounds 1, 4 and 5 showed acetylcholinesterase inhibitory activity, and compound 5 exhibited α-glucosidase inhibitory activity.Conclusion Compound 1 is a new compound named as crasscid A, and compounds 1-3 and 7 are obtained from agarwood for the first time.

Objective To investigate the clinical significance of the alteration of left atrium diameter in hypertension patients.Methods Among 85 hypertension patients,65 cases were selected from the patients of our hospital.20 healthy subjects were also included in our study.Ultrasonography imager was used to measure the internal diameter of left atrium(LAD),septal thickness(IVST),left ventricular posterior wall thickness(LVPWT),left ventricular ejection fraction(LVEF),left ventricular mass index(LVMI),E/A ratio and rest heart rate(RHR) for the analysis among them.Results LAD significantly enlarged in hypertension and normal LVMI patients compared with that of the control group(P

An absorbable hemostatic material based on polysaccharide was prepared. The concentration of blood cells and coagulation factors was increased by reducing the water content in the blood, so as to reduce the coagulation time and achieve the purpose of rapid hemostasis. The specific surface area of starch was increased by using hydrochloric acid to hydrolyze potato starch, which made it easier to combine with α-amylase and increased the degradation rate. Starch was crosslinked into microspheres by crosslinking agent, which made the particle size uniform and greatly improved the water absorption. The surface modification of crosslinked starch microspheres with carboxymethyl group can further improve the water absorption of hemostatic materials. The results showed that the water absorption rate of our hemostatic material was more than 800%, and the average hemostatic time in the animal model was 138.7s. Compared with the imported products on the market, our hemostatic material have better hemostatic performance.
Animals ; Hemostasis ; Hemostatics/pharmacology* ; Polysaccharides/pharmacology* ; Starch/pharmacology* ; Water/pharmacology*