Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Blood Circulation ; drug effects ; Cardiovascular Agents ; therapeutic use ; Cardiovascular Diseases ; prevention & control ; China ; Humans ; Medicine, Chinese Traditional

Academies and Institutes ; organization & administration ; China ; Combined Modality Therapy ; methods ; trends ; Drugs, Chinese Herbal ; therapeutic use ; Education, Medical, Continuing ; Humans ; Integrative Medicine ; legislation & jurisprudence ; trends ; International Cooperation ; Medicine, Chinese Traditional ; methods ; trends ; Western World

Cardiovascular Diseases ; Cardiovascular System ; Diet, Vegetarian ; Humans ; Integrative Medicine ; Vegetarians

Decision Making ; Esophageal Diseases ; diagnosis ; therapy ; Humans ; Physician's Role ; Thoracic Surgical Procedures

Drug Compounding ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Materia Medica

Drugs, Chinese Herbal ; administration & dosage ; standards ; Humans ; Injections, Intravenous ; Pharmaceutical Preparations ; administration & dosage ; standards ; Quality Control

Intestinal mucosa plays important roles in digestion, absorption and substance exchange between organism and external environment. Meanwhile, it is the largest immune organ and mucosal barrier, including mechanical, biological and immune barrier. A variety of diseases, especially postoperative complications, are associated with the damage of mucosal barrier. Esophageal cancer surgery is complex and many perioperative factors, especially hypoperfusion and fasting, may affect the integrity of intestinal barrier. Understanding of the mechanism of intestinal barrier (mechanical, biological and immune barrier), the physiological function of probiotics, and the benefit of early enteral nutrition to intestinal barrier are important components to achieve fast recovery after surgery for esophageal cancer.
Enteral Nutrition ; Esophageal Neoplasms ; surgery ; therapy ; Humans ; Intestinal Mucosa ; Perioperative Period

Humans ; Hypertension ; therapy ; Integrative Medicine ; Medicine, Chinese Traditional

Critical Pathways ; Integrative Medicine