Objective To investigate the effect of levothyrocine-thyroxine 4 (L-T4) replacement therapy on cognitive status in elderly patients with subclinical hypothyroidism (SCH).Methods One hundred and twenty elderly patients with SCH were selected and treated with thyroid hormone replacement therapy for 6 months.The thyroid functions and cognitive impairments were tested for all the subjects before and after the therapy.Results After 6 months of thyroid hormone replacement therapy,the level of thyrotropic hormone was significantly lower than that of before herapy((15.21 ± 1.78) mU/L vs.(3.46 ± 0.37) mU/L,t =6.417,P =0.0083));The neuropsyehological test performance in patients with hypothyroidism showed statistically significant improvements in clock drawing test,iconic memory,block design,complex graphics recall and delayed recall,trail making A,word list learningl,recall and recognize,forward and backward digital span,verbal fluency,and digital symbol from WAIS-RC ((8.74 ± 1.08) vs.(9.63 ± 1.32),(6.14 ± 1.03) vs.(8.47 ± 1.38),(30.36 ± 7.27) vs.(36.42 ±8.54),(13.71 ± 1.05) vs.(14.87 ± 1.38),(11.21 ± 1.13) vs.(13.56 ±1.34),(11.36 ± 1.27)vs.(13.06 ±1.47),(5.27 ±0.84) vs.(6.54 ±1.03),(63.13 ±12.09)vs.(54.63 ±10.07),(3.64 ± 1.07)vs.(4.91 ±1.32),(5.27 ±0.84) vs.(6.54±1.03),(8.52±1.16) vs.(9.83 ±0.96),(8.01 ± 0.83) vs.(8.94 ± 1.08),(4.04 ±0.57)vs.(5.37 ±0.75),(11.21 ± 1.03) vs.(13.43 ± 1.23),(33.46 ± 8.72) vs.(38.06 ± 9.58),t =13.907,11.027,12.093,16.374,10.361,11.536,8.794,-10.617,9.601,8.794,17.814,7.913,12.415,13.992,10.128 respectively,P =0.028,0.039,0.036,0.029,0.042,0.040,0.048,0.042,0.045,0.048,0.027,0.048,0.038,0.032,0.041 respectively).Conclusion The treatment of thyroid hormone for the edlerly patients with SCH has positive effects on theimprovement of mild cognitive impairment.

Spherical aberration is the major cause which affects the functional vision of the patients implanted with spherical intraocular lenses (IOLs). Aspheric IOLs are designed to eliminate the aberration like the nature lens, thus they can produce better visual quality. If it is feasible to customize IOL design to the individual cataract patient, aspheric IOLs may substitute the traditional spherical IOLs and become the trend of IOLs.

0.05);HIF-1?positive rate of control groups was higher than that in normal group and treatment group with significant difference among them(P0.05);VEGF positive rate in control groups was higher than that in normal group and treatment group with significant difference among them(P

Objective:To study the changes of metabolites in the acute cerebral infarction by 1HMRS and to identify the pre-sent time window and range of ischemic penumbra.Methods:Forty-four patients with hyperacute and acute cerebral infarctions were divided into five groups according to the time of attack.The normal side was taken as control.Routine MRI,DWI and PRESS multiple voxel acquisition were performed in all cases.The metabolites of NAA,Cr,Lac and the ratio of NAA/Cr and Lac/Cr were analyzed.Statistical analysis was performed by SPSS 13.0 software package,t-test and ANOVA analysis were used to detect the significance within each group and among different groups.Results:(1)Compared with counterpart,the decrease of NAA/Cr and the increase of Lac/Cr in cehtral region and posterior marginal region were significant inCompared with counterpart,the decrease of NAA and the increase of Lac in the acute lesions were significant(P0.05).But the significant differences were shown over time(P

Objective:To prepare doxorubicin liposome and investigate its effect on human MCF-7 in vitro. Methods:DOX liposome was prepared by(NH4)2SO4-gradient method.The MTT method was used to test cytotoxicity and reverse multidrug resistance of DOX liposome on MCF-7/DOX. Results:DOX was encapsulated by 90.77%. The results of liposome MTT experiment suggested that IC50 of free DOX was 4 times bigger than DOX liposome.The DOX liposomes’reversing effect on MDR was 4 times as much as that of free DOX. Conclusion:The method of(NH4)2SO4 gradient is suitable for producing DOX liposome and it has better cytotoxicity and reversing MDR effect on MCF-7 than free DOX.

Objective:To investigate the protection mechanism of adenosine A1 receptor agonist(2-chloro-N6-cyclopentyladenosine, CCPA)delayed preconditioning for rabbit myocardium during ischemia reperfusion.Methods:Thirty New Zealand male white rabbits were randomly assigned to Sham group,I/R group and CCPA group,with ten in each.Group CCPA was given CCPA 0.1 mg/kg before myocardial ischemia.Twenty-four hours later the I/R and CCPA underwent 40 min of coronary occlusion followed by 2 h of reperfusion.Blood samples were taken from arterial line at 20 min before occlusion(T)1,20 min after occlusion(T2),40 min after occlusion(T)3,1 h after reperfusion(T)4and 2 h after reperfusion(T)5for determination of the plasma levels of IL-10.At the end of the reperfusion,infarct size(IS)and area at risk(AAR)were defined by Evans blue and TTC staining.The heart was harvested and levels of the HSP70 were determined by Western blot analysis,and ultrastructures were observed under the electron microscopy.Results:The IL-10 and HSP70 levels of group CCPA was higher than those of group I/R.The CCPA(P

Objective:To investigate the effect of pcDNA3.1 vector on house keeping gene expression and set guide to further study work in using pcDNA3.1. Methods:5 cell lines were transfected with pcDNA3.1,48 h post transfection total RNA was extracted and applied for real time PCR to detect the RNA level of GAPDH、 ?-actin、18sRNA difference.Meanwhile,extract total protein and analyze the GAPDH and?-actin expression by Western blot.Results:RNA and protein levels all show of a decrease in 5 cell lines after transfection,with the most obviously ?-actin.Conclusion:pcDNA3.1 might have non specifinhibition effect to cell,when using pcDNA3. 1 for gene function research,it is not recommend to use ?- actin as reference gene.

Transjugular intrahepatic portosystemic stent shunt(TIPSS)was per- formed in 30 cases of liver cirrhosis with portal hypertention.Operations were susceeded in 29 cases with only one failure.Complications happened in 7 cases,In 29 patients the average blood bilirubin was 25.12?9.80 mmol/l before TIPSS turning to 50.46?34.50 mmol/l after TIPSS;The average blood amonia was 152.33?65.30 ?g/dl before TIPSS changing to 233.33?599.96 ?g/dl after TIPSS.The contributing factors were due to liver function state and shunting channel diameter for causing hepatic encephalopathy and jaundice.Other complications such as multipule liver abscesses,pneumonia,bacteriemia were related to the accompanied diseases and operative technique.The authors introduced the principles of pre- vention and treatment of complications,together with the indications and contraindications of TIPSS.

The study analyzes the major factors which h ave led to the differences between the current situation of drug research and de velopment in China and that in the developed countries: Long-time-existing pla nned economy has prevented Chinese medical enterprises from rapidly becoming the principal part in drug research and development; there is a lack of the entrepr eneurs who can establish the drug research and development systems and availa ble market environment; scientists and entrepreneurs have difficulty in cooperat ing well; the reorientation about the government functions leaves much to be desired Based on above factors, this study has developed related measureme nt s to reduce the differences, such as conducting the structural innovation; esta blishing the drug research and developments systems unalterably; rearranging the functions of the governments and optimizing the market environment; strengthen ing the cooperation among the excellent pharmceutical enterprises in China

Objective To study the natural history of HIV infection among paid blood donors(PBD).Methods Two thousand three hundred and sixty four individuals infected with HIV/AIDS via blood donation in twelve villages of Henan province were included in this study,Retrospective data were analyzed in combination with a cross-sectional survey.Results The number of deaths were 709 among these 2 364 subjects under study by the end of 2004,with a mortality rate of 30.1%.Among 712 deaths,32.8% were companied with serious lung infections,18.1% were complicated with serious diarrhea,8.2%-with central nervous infections,8.2%-with cirrhosis,6.6%-with various types of cancers,3% died of suicide and 23% died with unclear cause.Of them 544 AIDS patients died before the ARV treatment was available in rural areas of Henan at the end of 2002,with a mortality rate of 23.0%.The median survival period for individuals with HIV infection was 9.5 years,and the median incubation period was about 8.5 years.Conclusion The median aurvival period for individuals with HIV infection via blood donation in rural areas of Henan was 9.5 years and the median incubation period was about 8.5 years among them.