Rats were cauterized with moxa on the hips, and the subcutaneous and intramuscular tempetratures were kept at 45°C and at 40°C for 15min, respectively. Rats were killed by perfusion of sucrose solution under deep anesthesia and the muscle tissues of the hips were collected from the bodies at 0h, 3h and 24h after the moxibustion. The proteins were extracted from the homogenized and centrifuged tissues. Two dimensional gel electrophoreses of the proteins were carried out. Heat-shock proteins of molecular mass 70, 000 (hsp 70) and 71, 000 (hsp 71) were detected for the rat killed 3h after the moxibustion. However, no hsp 70 but hsp 71 was detected in the rats killed 0h and 24h after the moxibustion. Hsp 71 was detected in the control rat muscle as well.

Urethan-anesthetized rats were electrically stimulated for 15min by inserting acupuncture needles as electrodes into the muscle of the hips (5Hz, 3-5mA). Another rats were stimulated by moxibustion (moxa, 10mg, 10 times) for 15min. Both groups of rats were sacrificed and muscular tissues were excised 3 hours after the stimulation. The proteins extracted from the homogenized and centrifuged tissues were analyzed by two-dimensional gel electrophoreses. Stress proteins (hsp) with molecular weight of 70000 (p70), 85000 (p85) and 100000 (p100) were detected in both rats stimulated by electroacupuncture and moxibustion. Each ratio of p70, p85 and p100 to control proteins analyzed by personal image analyze system was 4.5%, 17.4% and 1.0% in electroacupuncture, 6.8%, 20.8% and 1.2% in moxibustion, respectively. The tendency of the ratios in both cases was almost similar.

We show here that stress proteins (Mr 70K, 85K and 100K) were induced by both electroacupuncture and moxibustion.
Rats (Wister strain, male, 250g) were cauterized with moxa on the hip, and the subcutaneous and intramuscular temperatures of the hip were kept at 45°C and at 39-40°C for 15 minutes. While, another group of rats were electrically stimulated for 15min inserting acupuncture needles as electrodes into the muscle of the hips in the depth of 1cm and using current pulse acupuncture system (5Hz, 3-5mA). Both groups of rats were sacrificed under deep anesthesia and the muscular tissues were excised 3 hours after the stimulations. The proteins extracted from the homogenized and centrifuged tissues in the two groups of rats and control rats were analyzed by two-dimensional gel electrophoreses. The heat shock proteins with molecular weight of 70000 (Mr 70K, hsp 70), 85000 (Mr 85K, hsp 85) and 100000 (Mr 100K, hsp 100) were detected in the rats stimulated by moxibustion. The stress proteins of molecular mass 70000, 85000 and 100000 similar to hsps induced by moxibustion were found in the rats stimulated by electroacupuncture.

Questionnaire survey were conducted three times to evalute the social life and functional problems in patients with orthotopic neobladder after cystectomy. A total of 34 responses were collected froml9 patients with a mean age of 66 years at diagnosis. The patients with orthotopic neobladder were found to be troubled with prolonged urinary incontinence such as nocturnal enuresis and stress incontinence. The problems was deteriorating the quality of life of the patients.

Approximately 14.1 million patients have dyslipidemia in Japan. Promotion of self-medication for the prevention of dyslipidemia is needed. After a change in law in 2014, residents of Japan are allowed to measure HbA1c, cholesterol, and triglyceride levels by self-blood sampling from the fingers under the guidance of pharmacists. In this study, we held an event to measure the lipid levels within a community and evaluated the usefulness of a simple measurement for the early detection of dyslipidemia. Furthermore, we surveyed community members’ knowledge of dyslipidemia by self-questionnaires. Of the 48 local residents who came to the event, 45 had their lipids measured and answered the questionnaires. In 12 applicants, HDL-cholesterol or non-HDL-cholesterol, which are not affected by meals, exceeded the standard range. In the questionnaire study, 89% of applicants selected arteriosclerosis as the main consequence of dyslipidemia. Additionally, over 82% of applicants selected blue-backed fish, dietary fiber, and soybeans as food items that inhibit the rise in lipid levels. However, only 31% of applicants recognized that fruits also affect lipid levels. Although it was disappointing that 60% of applicants did not know that they could measure HbA1c and lipids at community pharmacies, 62% desired measurement of those levels in the future. This study suggested that the simple lipid measurement is useful for the early detection of dyslipidemia but local residents need to be informed about it. Furthermore, improvement in the recognition of dyslipidemia by local residents is needed.

10 male and 8 female skillful tester on physical fitness measurements were attended. They were measured one another the body girth and blood pressure of themselves. The result obtained on the same person by the different tester were not always coincident. The following issues were obtained.
(1) Mean value of individual error finding on the systolic pressure was about 4mmHg, on the diastolic pressure was about 6mmHg. Mean value of the difference between the maximum and minimum value of the systolic pressure reported on the same person was 15.4mmHg in male group. That of the diastolic value was 21.4mmHg in male group. For the female group, that of the systolic pressure was 17.0mmHg, that of the diastolic pressure was 23.0mmHg.
(2) Mean value of individual error (as under, be showed by“error”) obtained on the chest girth was 1.43cm in male group, 1.05cm in female group. Mean value of the difference between the maximum and minimum value (as under, be showed by“breadth”) was 5.99cm in male, 4.08cm in female.
(3) “Error”observed on the overarm extended girth was 0.73cm in male, 0.79 cm in female, “breadth”was 2.96cm in male, 2.99cm in female.
(4) “Error”finding on the overarm flexed girth was 0.60cm for both sex, “breadth”was 2.81cm in male, 2.43cm in female.
(5) For the forearm girth, “Error”was 0.40cm in male. 0.24cm in female, “breadth”was 1.81cm in male, was 1.35cm in female.
(6) For the thigh girth, “Error”was 0.90cm in male, 0.69cm in female, “breadth”was 3.91cm in male, 2.93cm in female.
(7) On the calf girth measurement, in male and female group, “Error”was 0.44 and 0.40cm, “breadth”was 1.51 and 1.48cm respectively.
From above mentioned findings, we considered that“Error”is possible to regard as unavoidable error, “breadth”might be probable error.

OBJECTIVESTo prepare a dietary recommendation for young Japanese for the prevention of coronary heart disease, we surveyed the dietary habits, serum lipids, serum fatty acids and blood pressure levels among male and female students.

METHODSIn this study, 175 male and 246 female university students were interviewed for the frequency of consumption of selected food items and the habit of skipping breakfast. Serum fatty acids were analyzed by gas chromatography.

RESULTSThe frequencies of consumption of dishes cooked with oil and intake of vegetables were higher in female students than in male students. Serum n-6 polyunsaturated fatty acids were slightly higher in females students than in male students while that of serum n-3 polyunsaturated fatty acids was 6.4% in both sexes. For both males and females, those who skipped breakfast at least once a week were less likely to consume vegetables and fruits than those who did not. Female students who skipped breakfast were also less likely to consume fish and had lower composition of n-3 polyunsaturated fatty acid and higher systolic and diastolic blood pressure levels than males. For female, the intake of milk and dairy products correlated inversely with systolic blood pressure levels and the intake of n-3 polyunsaturated fatty acids correlated inversely with diastolic blood pressure levels. For males, the intake of fruits and consumption of dishes cooked with oil correlated inversely with systolic and diastolic blood pressure levels.

CONCLUSIONOur findings suggest that increased intake of fish, milk and dairy products, fruits and vegetables from early adulthood and a regular dietary pattern are important to reduce the risk factors for coronary heart disease.