1.Identification of a Novel Restriction Enzyme from Helicobacter Pylori that Recognzes and Clevages 5'GATC-3' 3'CTAG-5'.
Kazuhiko NAKAJIMA ; Tsuyoshi MORITA ; Hiromasa KOIZUKA ; Takashi SHIMOYAMA ; Toshihide TAMURA
Journal of the Korean Society for Microbiology 2000;35(5):376-376
No Abstract Available.
Helicobacter pylori*
;
Helicobacter*
2.Thrombomodulin Concentration in Patient Undergoing Surgery for Abdominal Aortic Aneurysm.
Takaaki Sugita ; Shoji Watarida ; Kazuhiko Katsuyama ; Yasuhiko Nakajima ; Rie Yamamoto ; Atsumi Mori
Japanese Journal of Cardiovascular Surgery 1997;26(2):87-89
Thrombomodulin is an endothelial cell membrane protein that is released into the blood in soluble forms in response to endothelial damage. We evaluated thrombomodulin in blood samples taken from the femoral vein before, during and after elective surgery for abdominal aortic aneurysm in 9 patients. Thrombomodulin was measured using an ETA assay. Thrombomodulin decreased significantly just before aortic declamp from before surgery (p<0.001), and remained unchanged just after and 1 hour after aortic declamp. Three hours after aortic declamp, thrombomodulin increased significantly from 1 hour after aortic declamp (p<0.01), peaked at 6 hours after aortic declamp and then decreased. These data suggest that thrombomodulin did not increase significantly during surgery for abdominal aortic aneurysm.
3.Clinical Experience with Recombinant Thrombomodulin in Patients Undergoing Cardiovascular Surgery Complicated by Disseminated Intravascular Coagulopathy
Hiroyuki Koike ; Atsushi Iguchi ; Hiroyuki Nakajima ; Kazuhiko Uebe ; Toshihisa Asakura ; Kozo Morita ; Masaru Kanbe ; Ken Takahashi ; Masahiro Ikeda ; Hiroshi Niinami
Japanese Journal of Cardiovascular Surgery 2013;42(4):267-273
Studies have shown that postoperative disseminated intravascular coagulopathy (DIC) occurs in some patients with cardiac disease, acute aortic dissection, and ruptured abdominal aortic aneurysm. The specific pathophysiology of DIC in these settings are related to low cardiac function, shock, infection and sepsis as well as activation of coagulation cascade in the aneurysm sac or dissected aorta. A soluble form of recombinant human thrombomodulin (rhsTM) was approved in 2008 for the treatment of DIC. This report describes the safety and efficacy of rhsTM for the treatment of DIC in patients with cardiovascular disease operated in our department. Between October 2010 and March 2012, 35 patients with postoperative DIC were treated with rhsTM. Diagnosis of DIC was based on the diagnostic criteria for DIC of the Japanese Association for Acute Medicine (JAAM). During the first 6 months of the study period, after a diagnosis of DIC was made, the patients were treated with gabexate mesilate and antithrombin III, and if patients showed no improvement with conventional treatment, they received rhsTM for 6 days. During the last 10 months of the study period, patients received rhsTM soon after a diagnosis of DIC was made. Twenty seven patients survived for 28 days after rhsTM treatment, and the mortality rate was 22.9% (8/35). Patients who survived showed improvement in acute phase DIC scores, FDP levels, D-Dimer, fibrinogen and platelet counts during rhsTM treatment, but no improvement was observed in patients who died. No serious adverse events were found up to 28 days after the start of rhsTM administration. In conclusion, this study showed no adverse events of rhsTM, and further studies are needed to confirm that rhsTM administration is an effective therapeutic modality in the management of DIC after cardiovascular surgery.
4.Anastomotic External Iliac Artery False Aneurysm Developing 15 Years Later at the Site of Peripheral Anastomosis of a Temporary Bypass
Yasuhiko Nakajima ; Takaaki Sugita ; Shoji Watarida ; Masahiko Onoe ; Takehisa Nojima ; Kazuhiko Katsuyama ; Ryoko Tabata ; Shuichi Matsuno ; Atsumi Mori
Japanese Journal of Cardiovascular Surgery 1995;24(4):268-271
Anastomotic false aneurysm (AFA) of the aorta or iliac artery is a rare but life-threatening complication of prosthetic grafts. We report a surgical case involving AFA of the right external iliac artery which developed at the site of peripheral anastomosis of the temporary bypass procedure used during prosthetic reconstruction of the descending aorta for dissecting aneurysm (DeBakey IIIb) 15 years previously. A 60-year-old woman was hospitalized with rapidly growing right lower abdominal mass. Computed tomography and angiography revealed that the mass was an anastomotic external iliac artery false aneurysm and surgery was performed. The AFA was exposed transperitoneally and resected with a part of the intact external iliac artery without complication. Anatomical reconstruction was completed with a prosthesis. The postoperative course was uneventful. We conclude that patients with retroperitoneal grafts require lifelong routine periodic follow-up and if an AFA is discovered, it should be resected.
5.Unusual Dilatation of Gelatin-Impregnated Knitted Dacron Prostheses after Abdominal Aortic Aneurysm Surgery.
Takaaki Sugita ; Shoji Watarida ; Masahiko Onoe ; Takehisa Nojima ; Kazuhiko Katsuyama ; Yasuhiko Nakajima ; Rie Yamamoto ; Ryoko Tabata ; Shuichi Matsuno ; Atsumi Mori
Japanese Journal of Cardiovascular Surgery 1995;24(6):363-367
We experienced unusual dilatation of gelatin-impregnated knitted Dacron prostheses after abdominal aortic aneurysm surgery. Therefore, we investigated dilatation of gelatin impregnated knitted Dacron grafts compared with other types of Dacron grafts after abdominal aortic aneurysm surgery. Eighteen grafts inserted after abdominal aortic aneurysm surgery were studied for to evaluate dilatation. Enhance CT was used to determine the external diameter of the most dilated portion of the abdominal aortic grafts and high speed plain CT was used to determine the most dilated internal diameter. The gelatin-impregnated knitted Dacron grafts dilated from 25% to 43.8% (mean 31.8±7.2%), significantly more than collagen impregnated woven Dacron grafts (p=0.0003). Moreover, high fever was frequently noticed after these grafts implantation (66.7%). Therefore, caution must be used concerning these implantation in aortic lesions and careful follow-up study should be performed after implantation.
6.The Physical Effects of Body Movement on Axillary-to-Femoral Artery Graft.
Rie Yamamoto ; Takaaki Sugita ; Shouji Watarida ; Masahiko Onoe ; Kazuhiko Katsuyama ; Yasuhiko Nakajima ; Ryoukou Tabata ; Shuuichi Matuno ; Astushi Mori
Japanese Journal of Cardiovascular Surgery 1996;25(2):109-112
We encountered an unusual disruption of an expanded polytetrafluoroethylene (EPTFE) axillofemoral bypass graft apart from that anastomoses. We suspected the possible robe of the physical effects of body movement provoking the disruption of the axillofemoral bypass graft and therefore examined the physical effect of body movement on the axillary-to-femoral artery graft in 15 healthy men. At the lower part of the graft, the physical effect was significantly stronger. The disruption of this axillary-to-femoral artery graft was associated with the physical effect of body movement.