The aim of the present study was to investigate whether personal hamstring muscular activation increased by landing with the trunk bent forward. First, 14 healthy female university students were instructed to perform normal drop landing from a 40 cm-high box on both legs. Second, subjects were directed to perform drop landing with the trunk bent forward. Using images obtained from a high-speed video camera, the upper center of mass was calculated in the sagittal plane for determining the differences in these landings. For 0.1 second after toe contact, the muscular activation of quadriceps femoris and hamstring muscles was analyzed using an electromyogram (EMG), and these findings were compared between the 2 types of landings. There was no significant difference in the activation of quadriceps femoris, but the activation tended to decrease during landing with the trunk bent forward. However, the activation of hamstring muscles increased significantly during landing with the trunk bent forward compared with that during normal landing. During landing with the trunk bent forward, the activation of hamstring muscles increased, and the activation of quadriceps femoris tended to decrease. Because contraction of hamstring muscles decreases tension in the anterior cruciate ligament, the findings of this study may help in the prevention of anterior cruciate ligament (ACL) injury.