Animals ; Bile Ducts ; cytology ; Cell Transdifferentiation ; Hepatocytes ; cytology ; Liver ; embryology ; metabolism ; Mice ; Mice, Inbred C57BL ; Vascular Endothelial Growth Factor Receptor-2 ; biosynthesis

OBJECTIVETo characterize the differential and proliferative activities of FLK-1(+) cells derived from mouse fetal liver.

METHODThe FLK-1(+) fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1(+) cells into the inferior pole of the spleen of nine-week-old male C57 BL/6 mice after two-thirds hepatectomy.

RESULTSIn response to growth factors, FLK-1(+) cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1(+) cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver.

CONCLUSIONThese results indicate that FLK-1(+) cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cell-based therapy.

Animals ; Cell Differentiation ; Cells, Cultured ; Fetus ; cytology ; Male ; Mice ; Mice, Inbred C57BL ; Myocytes, Smooth Muscle ; cytology ; metabolism ; Stem Cells ; cytology ; metabolism ; Vascular Endothelial Growth Factor Receptor-2 ; metabolism