1.Erratum: Abstract & Figure Correction. Effects of natural eggshell membrane (NEM) on monosodium iodoacetate-induced arthritisin rats.
Boo Yong SIM ; Ji Won BAK ; Hae Jin LEE ; Ji Ae JUN ; Hak Joo CHOI ; Chang Ju KWON ; Hwa Young KIM ; Kevin J RUFF ; Karsten BRANDT ; Dong Hee KIM
Journal of Nutrition and Health 2015;48(5):457-458
We made a mistake in presenting abstract & figure correction.
2.Effects of natural eggshell membrane (NEM) on monosodium iodoacetate-induced arthritis in rats.
Boo Yong SIM ; Ji Won BAK ; Hae Jin LEE ; Ji Ae JUN ; Hak Joo CHOI ; Chang Ju KWON ; Hwa Young KIM ; Kevin J RUFF ; Karsten BRANDT ; Dong Hee KIM
Journal of Nutrition and Health 2015;48(4):310-318
PURPOSE: The aim of this study is to investigate anti-arthritis activity using natural eggshell membrane (NEM). METHODS: NEM was administered at 52 mg/kg, 200 mg/kg, and 400 mg/kg to SD-Rat, where arthritis was induced by monosodium iodoacetate (MIA) at 3 mg. NO production in serum was measured using Griess reagent. Cytokines including IL-1beta, and IL-6 were measured by Luminex and PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP were measured by ELISA. The cartilage of patella volume was examined and 3-D high-resolution reconstructions of the cartilage of patella were obtained using a Micro-CT system. RESULTS: Production of NO, IL-1beta, IL-6, PGE2, MMP-2, MMP-9, TIMP-1, LTB4, and hs-CRP in serum was decreased, respectively, in comparison with control. The cartilage of patella volume increased significantly. In addition, the NEM group showed a decrease in the cartilage of patella, synovial membrane, and transformation of fibrous tissue. CONCLUSION: The results for NEM showed significant anti-arthritis activity. These results may be developed as a raw material for new health food to ease the symptoms mentioned above.
Animals
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Arthritis*
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Cartilage
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Cytokines
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Dinoprostone
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Enzyme-Linked Immunosorbent Assay
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Food, Organic
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Interleukin-6
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Leukotriene B4
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Membranes*
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Osteoarthritis
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Patella
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Rats*
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Synovial Membrane
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Tissue Inhibitor of Metalloproteinase-1