Bovine growth hormone (bGH) gene was expressed in an insect spodoptera frugiperda cell line using a Baculovirus, Hyphantria cunea nuclear polyhedrosis virus (HcNPV). The bGH gene in pbGH plasmid was sequenced and amplified by PCR technique with two primers containing NcoI sites. The bGH gene consisted of 654 bp (217 amino acid residues), the 5'-untranslated region of the cloned bGH cDNA contains 56 bp, and the 3'-untranslated region contains 145 bp and two pallindromic regions. The amplified bGH gene DNA fragment (654 bp) was inserted into the NcoI site of the pHcEVII vector, which was named pHcbGH. The pHcbGH transfer vector DNA and the wild type HcNPV DNA were cotransfected into s. frugiperda cells to construct a recombinant virus. Eight recombinant viruses were selected and named HcbGH. One clone, HcbGH-4-1 showed largest plaque size, therefore the recombinant virus was further studied. The multiplication patters of the recombinant HcbGH-4-1 was similar to that of the wild type HcNPV. The bGH gene DNA in the HcbGH-4-1 recombinant was confirmed by Southern lot hybridization. The amount of the bGH (217 amino acid residues, 21 kDa) produced in S. frugiperda cells infected with the HcbGH-4-1 recombinant was approximately 5.5 ng per ml (106 cells) by radioimmunoassay.
Baculoviridae* ; Cell Line ; Clone Cells ; DNA ; DNA, Complementary ; Growth Hormone* ; Insects ; Nucleopolyhedrovirus* ; Plasmids ; Polymerase Chain Reaction ; Radioimmunoassay ; Spodoptera

A clinical observation was made on 45 of 53 patients with bladder tumors seen at the Department of Urology, Chosun University Medical School during the period from January. 1973 to December, 1979. The following results were obtained: 1. During that period. 820 patients were hospitalized and there were 53 cases of bladder tumors giving a rate of 6.5% 2. There were 32 males and 13 females a ratio of 2.5: 1 and 71. 1% of the patients was over 50 years of age. 3. The most common symptom was grose hematuria (82. 2%) and the others were frequent urination (35. 6%), dysuria (31. 1%), painful urination (22. 2%) and lower abdominal pain (22. 2%). 4. 22 cases (48.9%) of the patients visited the hospital within three months after the appearance of the initial symptom and 75.6% within 1 year. 5. 24 cases (53. 3%) had multiple tumors and 21 cases (46.7%) single tumor, Regardless of origin, lateral wall was involved in 30 cases (66. 7%) and the trigone in 20 cases (44.4%). 6. IVP performed in 43 cases revealed normal upper urinary tract in 27 cases (62. 8%) and filling defect of the bladder in 40 cases (93. 0%). 7. The pathologic examination performed in 37 cases revealed transitional cell carcinoma in 29 cases (78. 4%). 8. The operation was performed in 25 cases: Partial cystectomy in 11 cases open resection with coagulation in 3 cases, TUR in 5 cases, radical or simple total cystectomy in 6 cases and ileal diversion only is 1 case. 9. Of the 14 cases possible in follow up study 10 (71.4%) survived over 1 year after treatment.
Abdominal Pain ; Carcinoma, Transitional Cell ; Cystectomy ; Dysuria ; Female ; Follow-Up Studies ; Hematuria ; Humans ; Male ; Schools, Medical ; Urinary Bladder Neoplasms* ; Urinary Bladder* ; Urinary Tract ; Urination ; Urology

No abstract available.
Hepacivirus* ; Hepatitis C Antibodies* ; Hepatitis C* ; Hepatitis* ; Prevalence*

Fourty three patients known to hnve cervical intraepithelial neoplasia were assigned to Papanicolaou smear in five different ways, i, e, A) cotion tipped wood applieator at the pasterior vaginal fronix and the ectocervix, B) cotton tipped wood applicator at the ectocervix and the endocervical canal, C) Cytohrush at the endocervical canal, D) Cervexbrusk and E) Cyto-spatula. At the completion of cytologic study, all the subjects were done colposcopically directed biopsy and/or ECC. To evaluate the false negative rates of the cytologic test, cervical cone biopsy or hysteectorny was performed on all the patient. The false negat,ive rates were 53.5% in A) 39.5% in B), 14,0% in C), l8.6% in D), 25.6% in E) and statistical differences occurect between each group, It was also found that bleedings were most frequently endountered in E) and the encocervical cells were most frequently appeared in C). With these results, it can he stated that the rate of negative encocervical cells in the srnear were correlated with the false negative rates of cervical cytology.
Biopsy ; Cervical Intraepithelial Neoplasia ; Cervix Uteri* ; Female ; Humans ; Papanicolaou Test ; Wood

Primary Aldosteronism is characterized by hypertension, hypokalemic alkalosis and increased aldosterone secretion. It is important to distinguish between adenoma and hyperplasia for the treatment of primary aldosteronism because the hypertension associated with the adenomatous form is more likely to respond to operative removal. We report a case of aldosterone producing adrenal adenoma which was diagnosed by postural stimulation test of aldosterone and abdominal computed tomography, and was treated by adrenalectomy. A week after surgery, the blood pressure and laboratory data returned to normal.
Adenoma ; Adrenalectomy ; Aldosterone ; Alkalosis ; Blood Pressure ; Hyperaldosteronism ; Hyperplasia ; Hypertension

No abstract available.

No abstract available.
Pleural Effusion*

The addition of a monthly course of progesterone decrease the incidence of endometrialhyperplasia and endometrial carcinoma. The progesterones used in hormonal replacementtherapy(HRT) differ markedly in their progesteronic, androgenic and even estrogenicactivities. These characteristics may influence both symptomatic and metabolic side effects.The purpose of this study was to examine effect of bone and lipid metabolism inpostmenopausal women treated with conjugated equine estrogens plus dydrogesterone.A total 131 postmenopausal women(surgical menopause=95, natural menopause=36)and not-treated postmenopausal women(control=22) were invited to participate in thisstudy. Patients were divided into groups which had received conjugated equine estrogen(CEE)0.625 mg/day 21-day-cycle each month(n=20), CEE 0.625 mg/day plus Dydrogesterone 10mg/day 10-day-cycle each month(n=111), and no treatment control group(n=20).Serum lipid and lipoprotein(Triglyceride, Total cholesterol, High density lipoprotein, Lowdensity lipoprotein) and serum osteocalcin, urinary Deoxypyridinoline were examined in allpatients.There were no significant differences in bone and lipid metabolism between CEE andCEE plus Dydrogesterone groups.In conclusion, Dydrogesterone may be used safely in postmenopausal women withoutMetabolic side effect.
Cholesterol ; Dydrogesterone* ; Endometrial Neoplasms ; Estrogens, Conjugated (USP)* ; Female ; Hormone Replacement Therapy ; Humans ; Incidence ; Lipid Metabolism ; Lipoproteins ; Metabolism* ; Osteocalcin ; Progesterone

No abstract available.
Fibronectins* ; Plasma* ; Pre-Eclampsia*

No abstract available.