Objective To research clinical effects of Sini powder combing with enema in treatment of chronic nonspecific ulcerative colitis.Methods132 patients with chronic nonspecific ulcerative colitis treated in our hospital from January 2015 to October 2016 were randomly divided into two groups,with 66 cases in each group.The control group was treated with enema,and observation group was treated with Sini powder combing with enema to analyse effect of Sini powder.Results①The total effective rate was 93.94% in the observation group,higher than control group 75.76%(P<0.05).②After treatment,serum CRP,IL-8 and TNF-ɑ level of observation group were (14.92±3.04)mg/L、(0.52±0.05)μg/L、(18.65±3.18)ng/L,lower than control group(23.04±3.31)mg/L、(0.75±0.08)μg/L、(25.59±3.62)ng/L(all P<0.05).③ ESR and PLT level of observation group were(11.16±2.27)mm/h、(220.6±15.8)×10.9/L,lower than control group(15.93±2.58)mm/h、(258.3±18.9)×10.9/L(all P<0.05).④The adverse reaction rate was 15.15% in the observation group,and 21.22% in the control group, the difference was not statistically significant.⑤ The recurrence rate was 23.81% in the observation group,lower than control group 39.06%(P<0.05).ConclusionSini powder can improve the effect in treatment of chronic nonspecific ulcerative colitis and has higher security.

BDDH (borderline developmental dysplasia of the hip) or BHD (borderline hip dysplasia) is a disease with more controversies in the following aspects, including definition, diagnosis and interventions. The proportion of radiographic BDDH is 3.5 times that of developmental dysplasia of the hip (DDH) in the asymptomatic general population, and can exceed 40% in specific cohorts including female collegiate athletes. Up till now, researchers have not reached a consensus on its definition. The diagnosis depends largely on personal history, physical examinations and radiographic examination. The interventions, including non-operative approaches, hip arthroscopy and periacetabular osteotomy, are used. Inappropriate treatment may exacerbate symptoms and accelerate hip joint degeneration. Recent studies on BDDH with regard to its definition, diagnosis and interventions were reviewed. Lateral centre-edge angle (LCEA) measured on AP pelvis was crucial but not enough in defining BDDH, for it only reflects lateral coverage of the hip while anterior and posterior coverage may vary dramatically. Given that hips with an LCEA less than 20 degrees associated with developing osteoarthritis, we recommended that LCEA between 20 to 25 degrees be used as the radiographic criterion in the primary screening. Further evaluation of symptoms and physical examinations should be follow to identify hip instability and/or impingement. Periacetabular osteotomy must be indicated for hips with predominant instability and it can be performed with or without hip arthroscopy which is aimed to address labral lesion or cam deformity on the femoral side. Hip arthroscopy is indicated for hip with predominant impingement symptoms. However, there is no consensus on the outcomes of the two procedures currently. Further studies are still required to confirm long-term results. Thus, it is imperative to choose the right indications for each procedure.

Total hip arthroplasty (THA) is a well developed technique to which the spatial orientation of acetabular component is crucial. Since the idea of "safe zone" being proposed, such idea has long been obeyed by hip surgeons but has also been challenged in recent years. This is because post-operative instability could happen even if acetabular components are placed within "safe zone" . The latest researches have demonstrated that sagittal spinal balance and spino-pelvic mobility can affect the spatial orientation of acetabular components. Factors including spinal degenerative changes, long lumbosacral changes and spinal ankyloses can lead to sagittal spinal imbalance and/or influence spino-pelvic mobility, which subsequently affect pelvic retroversion. Proper pelvic retroversion is a way to compensate for sagittal spinal imbalance and is beneficial to post-operative stability. It is necessary for hip surgeons to rethink "safe zone" before performing THA and understand spinal balance as well as spinopelvic mobility. The goal is to reduce the rate of dislocation. This review started by introducing the related spinopelvic parameters frequently seen in papers published recently and the definitions of acetabular cup orientations. After the spinal sagittal balance and its compensatory mechanisms session, discussed its clinical relevance, as well as the significance of spinal mobility, in spinopelvic motion under three different postures.

Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.
Aerobiosis ; Anaerobiosis ; Escherichia coli ; genetics ; metabolism ; Fermentation ; Glycerol ; metabolism ; L-Lactate Dehydrogenase ; metabolism ; Lactic Acid ; biosynthesis ; Promoter Regions, Genetic ; genetics ; Temperature

When Escherichia coli CICIM B0013-030 (B0013, ack-pta, pps, pflB) was used for D-lactate production, succinate and acetate were the main byproducts (as much as 11.9 and 7.1% the amount of lactate respectively). In order to decrease the byproduct levels, we inactivated succinate and acetate synthesis in B0013-030. Two recombinant plasmids containing mutation cassettes of frdA::difGm and tdcDE::difGm respectively were constructed first. The mutation cassettes were used to delete the target genes on the chromosomal by Red recombination. Subsequently, the antibiotic resistance gene was excised from the chromosomal by Xer recombination. Thereby, mutants B0013-040B (B0013-030, frdA) and B0013-050B (B0013-040B, tdcDE) were produced. D-lactate producing abilities of the engineered strains were tested both in shake flasks and in bioreactors using two-phase fermentation (aerobic growth and anaerobic fermentation) with glucose as the sole carbon source. When fermentation was carried out in shake flasks, inactivation of frdA in B0013-030 to produce B0013-040B reduced succinate accumulation by 80.8%. When tested in a 7-liter bioreactor, B0013-040B accumulated 114.5 g/L D-lactate of over 99.9% optical purity. However, 1.0 g/L succinate and 5.4 g/L acetate still remained in the broth. Further inactivation of tdcD and tdcE genes in B0013-040B to produce B0013-050B decreased acetate and succinate accumulation to 0.4 g/L and 0.4 g/L respectively, and lactate titer was as much as 111.9 g/L (tested in the 7-liter bioreactor). In lightof the lower byproduct levels and high lactate production, strain B00 13-050B may prove useful for D-lactate production.
Acetates ; metabolism ; Escherichia coli ; genetics ; metabolism ; Fermentation ; Genetic Engineering ; Lactic Acid ; biosynthesis ; Metabolic Networks and Pathways ; genetics ; Mutation ; Plasmids ; genetics ; Succinic Acid ; metabolism

Objective:To investigate the clinical outcomes of open reduction and cannulated screw fixation directly via the anterior approach for femoral neck fracture of Garden type Ⅳ which is difficultly reducible in young and middle-aged patients.Methods:Forty patients aged younger than 65 years old were treated by open reduction and cannulated screw fixation directly via the anterior approach for femoral neck fractures which were difficulty reducible at Department of Orthopaedics, Mindong Hospital from March 2015 to October 2017. They were 28 males and 12 females, aged from 22 to 62 years (mean, 49.2 years). All the fractures were type Ⅳ by the Garden classification. The time from injury to operation averaged 3.2 d (range, from 1 to 6 d). Their postoperative fracture nonunion and femoral head necrosis were recorded. The reduction quality and hip function were evaluated using Garden reduction index and Harris hip scores.Results:All the 40 patients were followed up for 11 to 44 months (mean, 22.3 months). Nonunion occurred in one patient who had to accept secondary artificial hip replacement. No avascular necrosis of the femoral head occurred in any patients. The anteroposterior Garden reduction indexes at 2 d after surgery and at the last follow-up for the 40 patients were respectively 156.97°±1.40° and 157.07°±1.47°, showing no significant difference( t=-0.302, P=0.765); so were the lateral Garden reduction indexes (171.82°±4.48° and 172.10°±4.27°) ( t=-2.333, P=0.817). The Harris hip scores at the final follow-up for the hip ranged from 77 to 98 (average, 92); 31 cases were rated as excellent, 6 as good, 2 as fair and one as poor, giving an excellent to good rate of 92.5%. Conclusion:In the treatment of femoral neck fracture of Garden type Ⅳ which is difficultly reducible in young and middle-aged patients, if closed reduction has failed, open reduction and cannulated screw fixation directly via the anterior approach can achieve satisfactory short-term outcomes because it facilitates fracture reduction and fixation under direct vision, promotes fracture union and reduces avascular necrosis of the femoral head.