Objective To investigate the expressions of transmembrane protein 8(Tspan8)and integrin α5(ITGA5)in breast cancer and their relationship with clinicopathological features and prognosis.Methods A total of 136 breast cancer patients admitted to the hospital from April 2018 to April 2020 were enrolled in the study.Breast cancer tissue samples and corresponding paracancerous tissue samples were collected.The ex-pressions of Tspan8 and ITGA5 in breast cancer tissues and corresponding adjacent tissues were detected by immunohistochemistry.The relationship between the expressions of Tspan8 and ITGA5 and the clinicopatho-logical characteristics of breast cancer patients was analyzed.The discharged breast cancer patients were fol-lowed up for 36 months,and the survival status of the patients was recorded.The 3-year survival rate of breast cancer patients with different clinicopathological characteristics was compared.Multivariate Cox regression was used to analyze the influencing factors of axillary lymph node metastasis in breast cancer patients.Results The positive expression rates of Tspan8 and ITGA5 in breast cancer tissues were higher than those in adjacent tissues(P＜0.05).The positive expression rates of Tspan8 and ITGA5 in breast cancer patients with poor differentiation,tumor maximum diameter ≥3 cm,TNM stage Ⅲ-Ⅳ,axillary lymph node metastasis and other molecular subtypes were higher than those in moderate/well differentiation,tumor maximum diam-eter＜3 cm,TNM stage Ⅰ-Ⅱ,no axillary lymph node metastasis and triple negative subtype(P＜0.05).The 3-year survival rate of patients with positive expression of Tspan8 and ITGA5 was significantly lower than that of patients with negative expression of Tspan8 and ITGA5(P＜0.05).The 3-year survival rate of patients with poor differentiation,TNM stage Ⅲ-Ⅳ,axillary lymph node metastasis,other molecular sub-types and positive expressions of Tspan8 and ITGA5 were lower than those of patients with moderate/high differentiation,TNM stage Ⅰ-Ⅱ,no axillary lymph node metastasis,triple negative subtype and negative ex-pressions of Tspan8 and ITGA5(P＜0.05).TNM stage Ⅲ-Ⅳ(HR=2.289,95％CI:1.519-3.447),other molecular subtypes(HR=2.622,95％CI:1.744-3.942),Tspan8 positive expression(HR=3.622,95％CI:2.159-6.077)and ITGA5 positive expression(HR=3.142,95％CI:2.022-4.884)were risk factors for ax-illary lymph node metastasis in breast cancer patients(P＜0.05).Conclusion Tspan8 and ITGA5 are highly expressed in breast cancer patients,which are related to the clinicopathological characteristics and prognosis of patients.

Background and Aims:Studies have shown that miR-199b-3p is downregulated in gastric cancer tissues,while cysteine-rich transmembrane BMP regulator 1(CRIM1)is upregulated in these tissues.However,the role and mechanism of miR-199b-3p in the biological behavior of gastric cancer cells are still unclear,as is its potential association with CRIM1.Therefore,this study was conducted to investigate whether there is an interaction between miR-199b-3p and CRIM1 and how they affect the function of gastric cancer cells. Methods:qRT-PCR and immunohistochemistry were used to detect the expression levels of miR-199b-3p and CRIM1 in gastric cancer tissues and adjacent non-cancerous tissues.Gastric cancer MGC803 cells were used to assess changes in cell proliferation,invasion/migration abilities,and apoptosis rates after overexpression of miR-199b-3p(using miR-199b-3p mimics)or knockdown of CRIM1(using si-CRIM1).Bioinformatics analysis was used to predict the targeting relationship between miR-199b-3p and CRIM1,which was further validated by dual-luciferase reporter assay and confirmed through Western blot analysis. Results:The results of qRT-PCR indicated that,compared to adjacent non-cancerous tissues,miR-199b-3p expression was significantly lower in gastric cancer tissues,while CRIM1 expression was higher(both P＜0.05).Immunohistochemistry results demonstrated positive expression of CRIM1 in cancerous tissues,while it was negative in non-cancerous tissues.Overexpression of miR-199b-3p or CRIM1 knockdown resulted in decreased proliferation and invasion/migration abilities of MGC803 cells,along with increased apoptosis rates(all P＜0.05).Bioinformatics prediction and dual-luciferase reporter assays confirmed that CRIM1 is a target of miR-199b-3p.Western blot analysis showed that CRIM1 expression was significantly reduced after transfection with miR-199b-3p mimics(P＜0.05). Conclusion:CRIM1 is a target gene of miR-199b-3p,which can inhibit the proliferation,invasion,and migration of gastric cancer cells while promoting apoptosis by targeting and regulating CRIM1 activity.The miR-199b-3p/CRIM1 pathway may serve as a potential therapeutic target for gastric cancer.