1.Protective effect of heart-fatty acid binding protein on lipopolysaccharide-induced cardiomyocyte damage.
Yi LI ; Kangkai WANG ; Yongfang JIANG ; Jun CHEN
Journal of Central South University(Medical Sciences) 2015;40(5):457-463
OBJECTIVE:
To observe the protective effect of heart-fatty acid binding protein (H-FABP) on lipopolysaccharide (LPS)-induced cardiomyocyte damage.
METHODS:
The cardiomyocytes were isolated and cultured from 1-3 days old neonatal rats. The specific siRNA or plasmid of H-FABP were transfected into cells to alter H-FABP expression, which was evaluated by Western blot and quantitative-PCR. LPS-induced cardiomyocyte damage and inflammation were estimated by detecting the contents of lactate dehydrogenase(LDH), TNF-α, and IL-1β as well as cell viability.
RESULTS:
LPS treatment induced inflammation and cell damage indicated by a decrease in cell viability and an increase in LDH, TNF-α and IL-1β in the medium. When H-FABP was downregulated by siRNA transfection, the LPS-induced inflammation and cell damage were augmented. In contrast, when H-FABP was overexpressed by pcDNA3.1-H-FABP transfection, the LPS-induced inflammation and cell damage were suppressed.
CONCLUSION
H-FABP protects cardiomyocytes from LPS-induced inflammation and cell injury.
Animals
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Animals, Newborn
;
Cell Line
;
Cell Survival
;
Down-Regulation
;
Fatty Acid Binding Protein 3
;
Fatty Acid-Binding Proteins
;
metabolism
;
Inflammation
;
metabolism
;
Interleukin-1beta
;
metabolism
;
L-Lactate Dehydrogenase
;
metabolism
;
Lipopolysaccharides
;
adverse effects
;
Myocytes, Cardiac
;
cytology
;
drug effects
;
RNA, Small Interfering
;
genetics
;
Rats
;
Transfection
;
Tumor Necrosis Factor-alpha
;
metabolism
2.Investigation on influencing factors of health literacy and analysis on health education demand based on mobile terminal among college students
Hua ZHANG ; Zhiling SUN ; Haixia GAO ; Yinfeng DONG ; Ke ZHAO ; Sainan WANG ; Kangkai CHEN ; Yu ZHUANG
Chongqing Medicine 2018;47(15):2055-2060
Objective To understand the health literacy status,influencing factors and demand carrying out the health education based on the mobile terminal among college students to provide a new idea for formulating and implementing the health literacy promotion plan for university students.Methods The multi-stage stratified cluster random sampling method was adopted to extract 1 949 students from partial universities in Nanjing City and conduct the investigation by adopting the Rapid Estimate System of Health Literacy and Health Education Demand Based on the Mobile Terminal Questionnaire of College Students.The influence factors were analyzed by using the univariate analysis and ordinal multi-classification Logistic regression analy sis.Results The university students with high,marginal and low health literacy level accounted for 31.3%,48.9% and 19.8% respectively.The proportions of the students with high health literacy in the four dimensions,including health knowledge,health behaviors,health skill and health status,were 57.9%,18.3%,61.0% and 44.5% respectively.The ordinal multi-classification Logistic regression analysis showed that the major influence factors affecting the health literacy of university students were gender,education background of mothers,majors,grades,electively taking the health education course.81.2 % of university students wanted to or urgently wanted to understand the health literacy knowledge.The main route getting the health literacy knowledge by the university students was network(83.8%).91.8% of students were willing to learn the health literacy knowledge through mobile terminal.87.1% of students believed that the WeChat was suitable to learn the health knowledge,81.3% of students hoped to use the WeChat platform for learning the health knowledge,73.8% of students hoped the Wechat to push the health knowledge with the modes of words combined with images and video.It was recommended that the health knowledge pushing for 1-3 times per week was suitable.Conclusion The health literacy of university students is at the marginal level.The health behaviors literacy is most lacking and the demand for the health education based on the mobile terminal is stronger.The health literacy intervention of university students should sufficiently apply the new media platform superiority and the emphasis should be placed on the populations of males,low education background mothers,non-medicine major,low grade and without electively taking the health education course.
3.Minimum quality threshold in pre-clinical sepsis studies (MQTiPSS): an international expert consensus initiative for improvement of animal modeling in sepsis
Huan CHEN ; Huali ZHANG ; Kangkai WANG ; Yongming YAO ; Xianzhong XIAO
Chinese Critical Care Medicine 2019;31(8):930-932
Preclinical animal studies precede the majority of clinical trials. While the clinical definitions of sepsis and recommended treatments are regularly updated, a systematic review of preclinical models of sepsis has not been done and clear modeling guidelines are lacking. To address this deficit, a Wiggers-Bernard Conference on preclinical sepsis modeling was held in Vienna in May, 2017. The goal of the conference was to identify limitations of preclinical sepsis models and to propose a set of guidelines, defined as the "Minimum Quality Threshold in Pre-clinical Sepsis Studies" (MQTiPSS), to enhance translational value of these models. A total of 31 experts from 13 countries participated and were divided into six thematic Working Groups: Study Design, Humane modeling, Infection types, Organ failure/dysfunction, Fluid resuscitation, and Antimicrobial therapy endpoints. As basis for the MQTiPSS discussions, the participants conducted a literature review of the 260 most highly cited scientific articles on sepsis models (2003-2012). Overall, the participants reached consensus on 29 points; 20 at "recommendation" and nine at "consideration" strength. This Executive Summary provides a synopsis of the MQTiPSS consensus. We believe that these recommendations and considerations will serve to bring a level of standardization to preclinical models of sepsis and ultimately improve translation of preclinical findings. These guideline points are proposed as "best practices" for animal models of sepsis that should be implemented. To encourage its wide dissemination, this article is freely accessible on the Intensive Care Medicine Experimental and Infection journal websites. In order to encourage its wide dissemination, this article is freely accessible in Shock, Infection, and Intensive Care Medicine Experimental.
4. Minimum quality threshold in preclinical sepsis studies (MQTiPSS): quality threshold for fluid resuscitation and antimicrobial therapy endpoints
Huan CHEN ; Huali ZHANG ; Kangkai WANG ; Yongming YAO ; Xianzhong XIAO
Chinese Critical Care Medicine 2019;31(11):1307-1316
As outlined in the