The MLN64 gene,which is localized in q12-q21 of the human chromosome 17,encodes a novel transmembrane protein containing 445 amino acids .The C-terminus of MLN64 shares significant homology with the steroidogenic acute regulatory (StAR)protein,while its N-terminal domain exhibits a function of targe-ting the protein to late endosomes .MLN64 is likely to be involved in cholesterol transport and synthesis of steroid hormones.MLN64 gene,coamplified with C -erbB-2,is overexpressed in certain breast carcinomas and exerts an influence on biological characteristics of breast cancer cells .The high levels of MLN 64 observed in some breast carcinomas could contribute to the growth and progression of these tumors through increased intratumoral steroido -genesis,and is considered as a predictive factor of breast cancer prognosis .

Objective To investigate therapeutic effect and molecular mechanism of RPS on Diabetic Nephropathy rats.Methods DN rats were induced by STZ injection and grouped into model group, low-dose RPS group, middle-dose RPS group, high-dose RPS group, Rosiglitazone group and normal group.24 h urine protein,kidney weight index,blood glucose level and TG, BUN and Scr level in normal and DN rats were detected.TGF-βlevel of serum of rats in all groups by ELISA were detected.mRNA and protein expression level of PPARγ,aP2 and GLUT4 by RT PCR and Western blot were also detected.Results DN rats were induced successfully because the 24 h urine protein, kidney weight index and the levels of blood sugar and TG, BUN,Scr l and TGF-βevels, and the mRNA level of PPARγ,aP2 and GLUT4 level in DN rats increased than normal group(P<0.05).The mRNA level of PPARγ,aP2 and GLUT4 level increased(P<0.05) and other indexes decreased(P<0.05) as the doses of RPS increasing.The therapeutic effects of Rosiglitazone group was better than high dose PRS group.The protein level of PPARγ, aP2 and GLUT4 in DN rat skeletal muscle were significantly lower than normal group(P<0.05), and RPS can increase their expression level obviously.Conclusion RPS has a dose-dependent therapeutic effect on DN rats by improving the expression level of related protein in PPAR gamma signaling pathways.

Aim To determine whether there existed melatonin receptor (MR) in adult hepatoma tissue and to observe its property of binding.Methods Immuno-histochemistry was used to detect MR and identify its subcellular distribution. Specific binding and kinetic analyses of melatonin receptor were measured by radioligand binding assay.Results The results of immunohistochemistry showed that MT1 and MT2 existed in membrane, cytosol and nucleus of the hepatoma cells, but the expression of them principally localized in the membrane and cytosol. The specific binding assay properties of MR were presented as follows: the maximum binding capacity (B_(max)) was (0.29±0.07) pmol·g~(-1) protein. Equilibrium dissociation constant (K_d) was (48.7±6.5) pmol·L~(-1). The trait of MR, saturation and reversibility, was detected by ~(125)I-Mel specific binding kinetic analyses.Conclusions MR exists in adult hepatoma cells, furthermore, the subtypes of MR (MT1 and MT2) coexist in the membrane and cytosol, respectively,whose characters of the specific binding sites were low binding capacity,high affinity,saturation and reversibility.

Humans ; Medicine, Chinese Traditional ; Stomach Neoplasms ; therapy

Cerebellum connects with each part of the auditory pathway directly or indirectly, forming the anatomical basis for a cerebellar role in auditory process. Functional imaging studies and clinical observations provide more abundant support for this view. Tinnitus is one of the most common clinical manifestations when the auditory system is impaired and remains unsolved. Basic science researches in neurotransmitter chemistry and molecular biochemistry, together with functional imaging studies, indicate that cerebellum might contribute to the pathophysiology of tinnitus. In this review we summarize the current understanding of cerebellar role in auditory process and tinnitus.
Auditory Pathways ; Cerebellum ; physiopathology ; Humans ; Tinnitus ; physiopathology

Objective To investigate the status of report appropriateness in Clinical Hematology and to identify its potential influence factors to improve the quality of laboratory reports.Methods 1 120 National External Quality Assessment ( EQA ) participants laboratories were enrolled in this study.The questionnaires were assigned to all the participants in both electronic and printed form.The participants were asked to retrospectively count the total reports and the affected reports while the following quality indicators occurred in 2012:report delay, inconformity of data between instrument and LIS as well as between report and request erroneous report transportation, report recall, and report modification.All quality indicators were evaluated in two ways:percentage and sigma (σ) scale.Mann-Whitney Test and Kruskal-Wallis Test were used to analyze the potential impacts of report appropriateness.Results Totally 609 (54.38%)laboratories submitted the survey results .The sigma metrics of six quality indicators on report appropriateness were more than 4.The main reason for report delay was equipment failure.Group comparison suggested that the report appropriateness of laboratories in tertiary-A hospitals and accredited by ISO 15189 werebetter.Report appropriateness with electronic request, electronic form and electronic transmission were superior than that inother ways. Conclusions Report appropriateness in China differs from laboratory to laboratory. Laboratories should develop emergency action plan in case the instrument breaks down and strengthen information technology .Long-term internal quality control and external quality assessment scheme are very meaningful to identify the insufficient performance and improve laboratory quality .(Chin J Lab Med, 2015,38:632-636)

Objective:To explore the influential factors of cognitive change among Chinese oldest-old.Method: Three waves of data from the Chinese Longitudinal Healthy Longevity Survey(CLHLS)were analyzed with a two-level repeated measures model.Results:In baseline,the male had a higher mean MMSE score than the female(27.0?3.7/24.4?5.6,P

With the development of modern medical research , it has been found that there are many forms of cell death under lots of physiological or pathological conditions .Among them, necrosis and apoptosis are the 2 main forms of cell death.In recent years, varieties of new cell death patterns, such as autophagy, oncosis and paraptosis, have been discovered.In 2012, Dixon et al first discovered and reported a new form of iron-dependent cell death , ferroptosis.In this review, we try to expound the morphological and biochemical features of ferroptosis and its related diseases in detail , so as to deepen our understanding of cell death , thus providing new ideas for the prevention and treatment of clinically relevant diseases .

Objective:To clarify the effects of the BMP receptor inhibitor LDN-193189 in the dedifferentiated chondrosarcoma (DDCS) cell line NDCS-1 and to explore the anti-tumor mechanism of LDN-193189 in DDCS. Methods:NDCS-1 was treated with 5 nmol/L of LDN-193189. MTT assay and clone formation experiments were used to verify that LDN-193189 suppressed cel proliferation. Transwel and wound healing tests were performed to demonstrate that LDN-193189 inhibited cell invasion. Western blot detection was used to show that LDN-193189 inhibited the suppression of BMPR2, p-Smad1/5, and RUNX2 protein expression. Results:The BMPR2 signaling pathway was inhibited by LDN-193189;thus, cell viability and invasion were significantly suppressed. Conclusion:LDN-193189 induces the inhibition of progression in vitro via the BMPR2-p-Smad1/5-RUNX2 signaling pathway in the human DDCS cell line NDCS-1.

50% was seen in 7 cases after TACE and PEI(30%),and the 1?3?5 year′s survival rate was 52%?35%? and 9%,respectively.Conclusions Adjuvant chemotherapy was hardly beneficial to the treatment of liver cancer with parasitic blood supply after TACE, while PEI enhanced effect of TACE as a supplementary therapy as judged by long term survival. The change of sIL-2R, AFP level was a sensitive indicator for the outcome of the treatment.