1.Expression of Green Fluorescent Protein in Both Spodoptera frugiperda Cells and Bombyx mori Larvae by Ac-Bm Hybrid Virus.
Byung Rae JIN ; Hyung Joo YOON ; Eun Young YUN ; Seok Woo KANG ; Eun Sook CHO ; Seok Kwon KANG
Journal of the Korean Society of Virology 1998;28(3):225-232
We have expressed GFP in Sf9 and Bm5 cells or Bombyx by larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of Ac-Bm hybrid virus expressing P-galactosidase was cotransfected with baculovirus transfer vector containing GFP gene, pBacPAK-GFP in Sf9 cells. The Ac-Bm hybrid virus harboring GFP was named as Ac-Bm hybrid virus-GFP. The Ac-Bm hybrid virus-GFP-infected insect cells were easily selected by detecting the emission of GFP from each well of cell culture dish on the UV illuminator. GFP produced by Ac-Bm hybrid virus-GFP in Sf9 and Bm5 cells or B. mori larvae was confirmed by SDS-PAGE and Western blot analysis using GFP antibody. In addition, B. mori larvae infected with Ac-Bm hybrid virus-GFP was apparently appeared fluorescence from the whole body at 5 days postinoculation. The fluorescence of GFP from the hemolymph and fat body of B. mori larvae infected with Ac-Bm hybrid virus-GFP was also observed by fluorescence microscope. In conclusion, our results demonstrated that in baculovirus expression vector system, use of Ac-Bm hybrid virus have an additional advantage of expanded host range for producing recombinant proteins.
Animals
;
Baculoviridae
;
Blotting, Western
;
Bombyx*
;
Cell Culture Techniques
;
DNA
;
Electrophoresis, Polyacrylamide Gel
;
Fat Body
;
Fluorescence
;
Hemolymph
;
Host Specificity
;
Insects
;
Larva*
;
Recombinant Proteins
;
Sf9 Cells
;
Spodoptera*
2.Myositis ossificans traumatica IN MASSETER MUSCLE.
Sang Rae LEE ; Jae O CHO ; Yoon Goo KANG
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2001;27(4):358-361
We had experienced a case of traumatic myositis ossificans arising in right masseter muscle, inferior to zygomatic arch occurred on 25 year old male. He had some trauma on the site one year ago and visited with complaint of mouth opening limitation and swelling. Palpable mass with facial disfigurement was noted. and several ovoid radiopaque masses were revealed on C.T. examination. On histopathologic examination, multiple bone trabeculae with osteoblasts in its periphery was noted in connective tissues and invaded to neighbouring muscles, but any chondroid components were not revealed.
Adult
;
Connective Tissue
;
Humans
;
Male
;
Masseter Muscle*
;
Mouth
;
Muscles
;
Myositis Ossificans*
;
Myositis*
;
Osteoblasts
;
Zygoma
3.Clinical study of 53 patients requiring open thoracotomy after thoracic injuries.
Gyu Man KIM ; Kang Rae CHO ; Hyung Ryul LEE ; Jong Won KIM ; Sung Kwang LEE ; Hwang Kiw CHUNG
The Korean Journal of Thoracic and Cardiovascular Surgery 1991;24(11):1115-1124
No abstract available.
Humans
;
Thoracic Injuries*
;
Thoracotomy*
4.Extralobar pulmonary sequestration associated with esophageal fistula, diaphragmatic hernia and pyloric stenosis: a case report.
Young Sik PARK ; Kyu Wan PARK ; Pyung Rae CHO ; In Seug KANG ; Myung Ho BYUN ; Sook Nyoe LEE
Journal of the Korean Surgical Society 1991;40(4):536-544
No abstract available.
Bronchopulmonary Sequestration*
;
Esophageal Fistula*
;
Hernia, Diaphragmatic*
;
Pyloric Stenosis*
5.Malignant gastric leiomyoma.
Chang Joon AHN ; Cho Hyun PARK ; Jong Seo LEE ; Joon Gi KIM ; Sang Yong CHOO ; Rae Sung KANG ; In Chul KIM
Journal of the Korean Surgical Society 1993;45(2):199-208
No abstract available.
Leiomyoma*
6.Whole blood cyclosporine measurement by fluorescence polarization immunoassay.
Chan Jung PARK ; Kyung Ryung KANG ; Hyun Chan CHO ; Kyung Hwa LEE ; Hong Rae CHO ; Young Joo LEE ; Yoo Sun KIM ; Eun Mi LEE ; Ki Il PARK
The Journal of the Korean Society for Transplantation 1993;7(1):77-82
No abstract available.
Cyclosporine*
;
Fluorescence Polarization Immunoassay*
;
Fluorescence Polarization*
;
Fluorescence*
7.Optimal Cut-Off Value of the Coracohumeral Ligament Area as a Morphological Parameter to Confirm Frozen Shoulder
Hyung Rae CHO ; Byong Hyon CHO ; Keum Nae KANG ; Young Uk KIM
Journal of Korean Medical Science 2020;35(15):e99-
Background:
Thickened coracohumeral ligament (CHL) is one of the important morphological changes of frozen shoulder (FS). Previous research reported that coracohumeral ligament thickness (CHLT) is correlated with anterior glenohumeral instability, rotator interval and eventually FS. However, thickness may change depending on the cutting angle, and measurement point. To reduce measurement mistakes, we devised a new imaging criteria, called the coracohumeral ligament area (CHLA).
Methods:
CHL data were collected and analyzed from 52 patients with FS, and from 51 control subjects (no evidence of FS). Shoulder magnetic resonance imaging was performed in all subjects. We investigated the CHLT and CHLA at the maximal thickened view of the CHL using our picture archiving and communications system. The CHLA was measured as the whole area of the CHL including the most hypertrophied part of the MR images on the oblique sagittal plane. The CHLT was measured at the thickest point of the CHL.
Results:
The average CHLA was 40.88 ± 12.53 mm2 in the control group and 67.47 ± 19.88 mm2 in the FS group. The mean CHLT was 2.84 ± 0.67 mm in the control group and 4.01 ± 1.11 mm in the FS group. FS patients had significantly higher CHLA (P < 0.01) and CHLT (P < 0.01) than the control group. The receiver operator characteristic analysis showed that the most suitable cut-off score of the CHLA was 50.01 mm2, with 76.9% sensitivity, 76.5% specificity, and area under the curve (AUC) of 0.87. The most suitable cut-off value of the CHLT was 3.30 mm, with 71.2% sensitivity, 70.6% specificity, and AUC of 0.81.
Conclusion
The significantly positive correlation between the CHLA, CHLT and FS was found. We also demonstrate that the CHLA has statistically equivalent power to CHLT. Thus, for diagnosis of FS, the treating physician can refer to CHLA as well as CHLT.
8.Event-Related Functional Magnetic Resonance Imaging in Post-Traumatic Stress Disorder.
Jong Ik PARK ; Jin Pyo HONG ; Young Rae CHO ; Ji Kang PARK ; Ho Gyu LEE ; Chang Yoon KIM ; Oh Su HAN
Journal of Korean Neuropsychiatric Association 2002;41(4):649-659
OBJECTIVE: Symptom provocation paradigms using event-related functional magnetic resonance imaging(fMRI) can be an important tool to visualize neuroanatomic correlates of PTSD symptoms. The purpose of this study is to measure regional changes of brain upon exposure to fear stimulus and elucidate the probable relation between signal changes and fear response in PTSD. METHOD: Event-related fMRI was performed during a task where traffic accident-related photos and checkerboards were presented in 9 women with PTSD and 9 woman normal controls in unpredictable order. MRI data were acquired on a 1.5 T GE vision system with a head volume coil. Stimuli were presented on a mirror mounted on the head coil. A total of 200 functional images were taken during a 10-minute scanning session. TR was 3 seconds and inter-stimulus time was varing 4.5 to 11.5 seconds. Data was analyzed using SPM99. RESULTS: In PTSD group, the fear conditions versus the neutral conditions showed activations in both occipital cortex, both fusiform gyrus, left parietal lobule, both insula, right cerebellar tonsil, right putamen, right claustrum, but deactivations in both prefrontal gyrus(p<0.001). In normal control group, activation were found for the fear conditions as compared with the neutral conditions in left fusiform gyrus, both occipital cortex, left parietal lobule, right frontal lobule(p<0.001). CONCLUSIONS: Emotion provocation paradigm using event-related functional magnetic resonance imaging can be applied to illustrate fear response mechanism in PTSD. The result suggests that insula, limbic lobe, cerebellum may play a role in mediating fear response in PTSD.
Basal Ganglia
;
Brain
;
Cerebellum
;
Female
;
Head
;
Humans
;
Magnetic Resonance Imaging*
;
Negotiating
;
Palatine Tonsil
;
Putamen
;
Rabeprazole
;
Stress Disorders, Post-Traumatic*
9.A case of lymphocytic interstitial pneumonitis.
Hee Jin JUNG ; Eun Rae CHO ; Jae Jung SHIM ; Kwang Ho IN ; Sae Hwa YU ; Kyung Ho KANG ; Nam Hee WON ; Young Ho CHOI
Tuberculosis and Respiratory Diseases 1993;40(5):602-609
No abstract available.
Lung Diseases, Interstitial*
10.Clinical experience with vascular surgery.
Hyun Kyung KIM ; Gyu Man KIM ; Kang Rae CHO ; Hyung Ryul LEE ; Jong Won KIM ; Sung Kwang LEE ; Hwang Kwi CHUNG
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(12):1570-1577
No abstract available.