Objective To study the extraction technology of essential active components in compound shark cartilage capsules.Methods Astragalin and matrine,the essential active components in compound shark cartilage capsules,were taken as the test indexes.The extraction was optimized by using orthogonal design.Results The calibration curve for astragalin and matrine was linear over the range of 1～5?g and 2.2～11.0?g,respectively.The results suggested that the optimum conditions:Astragalus membranaceus Bge.∶water =1∶16,extracting for 2h,repeating 3 times for extraction of astragalin;Sophora flavescens Ait.∶water=1∶16,extracting for 2h for extraction of matrine.Conclusion The extraction technology was simple,reliable and could be used in guality control of extracting the essential active components in compound shark cartilage capsules.

0.05).Conclusion The significantly reduced serum IGFBP-3 level is helpful for the diagnosis of HCC,especially in patients without chronic hepatitis and cirrhosis.

Objective To investigate the inhibitory effect of downregulation of hepatitis B virus (HBV) core gene (HBcAg) expression by RNA interference and magnetic nanoparticles on both HBV DNA replication and expression in vitro. Methods HepG2 2.2.15 cells were transfected with U6 promoter plasmids coding for small interfering RNA (siRNA) targeting HBV core gene using magnetic nanoparticles. RT-PCR and Western blot were used to assess the mRNA and protein expression HBV core antigen. Real-time PCR was used to evaluate the suppression efficiency of HBV-DNA replication and expression; and radioimmunoassay was used for HBV surface antigen (HBsAg), core antigen (HBcAg), and e antigen (HBeAg) detection. Results We successfully constructed nanoparticles with siRNA plasmid targeting HBV core antigen; HBcAg mRNA and HBV core antigen protein levels were significantly reduced in the transfected cells. HBV-DNA downregulation was estimated at 4-5 logs and the HBsAg and HBeAg levels were also reduced compared with the controls. Conclusion Downregulation of HBV core gene using RNAi technology and magnetic nanoparticles can potentially be used as a therapeutic strategy for Hepatitis B.