1.Efficacy Observation of Tuina Therapy for Fibromyalgia Syndrome
Kaiyong ZHANG ; Yang YANG ; Siwei XU ; Hongsheng ZHAN ; Bimeng ZHANG
Journal of Acupuncture and Tuina Science 2014;(6):383-388
Objective: To observe the efficacy of tuina manipulations for fibromyalgia syndrome (FMS). <br> Methods:Sixty eligible FMS patients were randomized into two groups, 30 in each group. Patients in the observation group were intervened by tuina manipulations to the corresponding segment of the spine based on the detection of disorders of the spine-related bones and tendons;patients in the control group were directed to practice functional exercises. Before and after intervention, the fibromyalgia impact questionnaire (FIQ) and visual analogue scale (VAS) were adopted for evaluation. <br> Results:After intervention, the FIQ and VAS scores dropped significantly in both groups (all P<0.05), and there were significant differences between the two groups (both P<0.05). The total effective rate was 86.7%in the observation group versus 63.3% in the control group, and the difference was statistically significant (P<0.05). <br> Conclusion:Spinal tuina manipulations are effective and safe in treating FMS, for significantly promoting the quality of life of the FMS patients.
2.Determination of Copper Ion in Rabbit with Implantation of Copper Intrauterine Device
Xiaoling XU ; Kaiyong HE ; Wangping PAN ; Xiaojun LYU ; Yuanhua HU
Herald of Medicine 2017;36(6):614-616
Objective To develop a method for determination of serumal copper ion in rabbit with implantation of copper intrauterine device.Methods At different time points after implantation,the serumal copper ion concentrations were determined by microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS) using scandium(Sc) and indium(In) as internal standards to compensate sample matrix effects,and the pharmacokinetics parameters were calculated in order to reflect copper ion release and metabolic rule.Results The serumal copper ion concentrations were kept at a low and stable level.The recoveries were in the range of 98.7%-113.3%,with the relative standard deviations of less than 5.0%.Conclusion The analytical method is simple,fast,and can be used for the determination of serumal copper ion in rabbit.
3.Acupuncture for dry eye syndrome: a meta-analysis of randomized controlled trials
Huiru JIANG ; Sujun LIU ; Peng LIU ; Siwei XU ; Yang YANG ; Kaiyong ZHANG ; Yin SHOU ; Bimeng ZHANG
Journal of Acupuncture and Tuina Science 2017;15(4):263-269
Objective: To summarize and critically assess the evidence from randomized controlled trials (RCTs) of acupuncture in treating dry eye syndrome (DES) according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the Cochrane Collaboration recommendations. Methods: A search of PubMed, the Cochrane Central Register of Controlled Trials, ClinicalTrials.gov and Embase was made from their inception to August 2016, as well as Chinese, Japanese, and Korean databases. Two reviewers independently selected RCTs and assessed the methodological quality. Meta-analysis and the level of evidence were processed by RevMan 5.3 and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. Results: After selection, 8 trials were subjected to our systematic review. The methodological quality was low generally. The 3-10 weeks follow-up showed that acupuncture improved the tear film break-up time (BUT) (MD=1.33, 95%CI=1.01-1.66, 619 participants). The mean difference of Schirmer's test was 1.73 mm (95%CI=1.28-2.18, 618 participants) between the acupuncture group and the control group. The subjective variables exhibited no significant differences. Conclusion: The low methodological quality of the trials does not suggest drawing firm conclusions on the value of acupuncture therapy for DES. Acupuncture treatment may have some effects on the tear film BUT and Schirmer's test, but not on the subjective symptoms. Well-planned large-scale high-quality RCTs are needed to make it clear whether acupuncture is effective in treating DES.
4.Warm needling moxibustion plus functional exercises for knee osteoarthritis
Kaiyong ZHANG ; Yang YANG ; Yin SHOU ; Siwei XU ; Huiru JIANG ; Bimeng ZHANG
Journal of Acupuncture and Tuina Science 2016;14(6):412-415
Objective:To observe the clinical efficacy of warm needling moxibustion plus functional exercises in treating knee osteoarthritis (KOA).Methods:Totally 108 eligible KOA patients were intervened by warm needling moxibustionplus functional exercises. The warm needling moxibustion was given 3 times a week, successively for 4 weeks. The visual analogue scale (VAS) was adopted to evaluate the pain intensity of the knee joint. After 4-week treatment, the change of VAS score, range of motion (ROM) of knee, and Lysholm score were observed, and the clinical efficacy was also estimated. Results:After treatment, the VAS score dropped obviously, and the ROM and Lysholm score increased markedly; the total effective rate was 83.3%. Conclusion:Warm needling moxibustionplus functional exercises is effective in treating KOA, as it can relieve the knee joint pain and improve the joint motion.
5.Clinical observation on warm needling moxibustion for erectile dysfunction
Peng LIU ; Sujun LIU ; Siwei XU ; Yang YANG ; Yin SHOU ; Kaiyong ZHANG ; Huiru JIANG ; Bimeng ZHANG
Journal of Acupuncture and Tuina Science 2017;15(1):59-62
Objective:To observe the therapeutic effect of warm needling moxibustion for erectile dysfunction (ED).
Methods:A total of 46 eligible ED patients were randomized into a treatment group (n=24) and a control group (n=22). The treatment group was intervened by warm needling moxibustion while the control group by conventional acupuncture treatment, for a total of 4 courses. The therapeutic effect was evaluated by the international index of erectile function 5 (IIEF-5).
Results:After 4 courses of treatment, the overall effective rate was 91.3% in the treatment group (23 completed), versus 75.0% in the control group (20 completed), with a significant difference (P<0.05). After the treatment, the change of IIEF-5 score in the treatment group was significantly different from that in the control group (P<0.05).
Conclusion:Warm needling moxibustion can produce a more significant efficacy than conventional acupuncture in the treatment of ED.
6.Effect of Electroacupuncture on Cartilage Expression of TypeⅠ Collagen Gene in a Rat Model of MIA-induced Knee Osteoarthritis
Ying SHOU ; Miaomiao LIU ; Junmei ZHOU ; Sujun LIU ; Siwei XU ; Yang YANG ; Kaiyong ZHANG ; Bimeng ZHANG
Shanghai Journal of Acupuncture and Moxibustion 2016;35(9):1119-1121
Objective To investigate whether inhibiting the expression of typeⅠ collagen gene is one of the mechanisms of action of electroacupuncture in improving knee osteoarthritis.Methods Forty male adult SD rats were randomized into normal, model, medication and electroacupuncture group, 10 rats each. A rat model of MIA-induced knee osteoarthritis was made by injecting monomer sodium iodoacetate (MIA) and driving rat movement. After model making, the medication group received an oral gavage of celecoxib dissolved in 10% DMSO and the electroacupuncture group, electroacupuncture at points Zusanli and Yanglingquan. Pain thresholds and the levels of cartilage expression of typeⅠ collagen mRNA were compared between various groups of rats before and after treatment.Results There was a statistically significant difference in pain threshold between the model, medication or electroacupuncture group of rats and the normal group after model making (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in pain threshold between the medication and andelectroacupuncture groups of rats before sacrifice (P>0.05). There was a statistically significant difference in the expression of typeⅠ collagen mRNA between the model, medication or electroacupuncture group of rats and the normal group (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in the expression of typeⅠ collagen mRNA between the electroacupuncture and medication groups of rats before sacrifice (P>0.05).Conclusion The mechanisms of actions of electroacupuncture and medication in treating knee osteoarthritis may be related to inhibiting the expression of typeⅠ collagen mRNA.
7.Clinical observation of acupuncture combined with sitting-position knee-adjustment manipulations for patellofemoral arthritis
Kaiyong ZHANG ; Peng LIU ; Siwei XU ; Bimeng ZHANG ; Hongsheng ZHAN ; Jue HONG
Journal of Acupuncture and Tuina Science 2022;20(6):476-481
Objective: To observe the efficacy of acupuncture combined with sitting-position knee-adjustment manipulations in treating patellofemoral osteoarthritis. Methods: Ninety-two patients with patellofemoral osteoarthritis were randomized into an observation group and a medication group, with 46 cases in each group. The observation group received acupuncture and sitting-position knee-adjustment manipulations, and the medication group received oral celecoxib capsules. After 8-week treatment, changes in the short-form McGill pain questionnaire (SF-MPQ) and Lysholm knee scoring scale (LKSS) scores were observed, and the clinical efficacy was compared.Results: The total effective rate was 87.0% in the observation group and 63.0% in the control group; the between- group difference was statistically significant. Before treatment, there were no significant differences in the SF-MPQ score or LKSS score (P>0.05). After 8-week treatment, the SF-MPQ [including pain rating index (PRI), visual analog scale (VAS), and present pain intensity (PPI)] and LKSS scores showed notable changes in both groups (P<0.05); the SF-MPQ and LKSS scores in the observation group were significantly different from those in the control group (P<0.05).Conclusion: Combining acupuncture and sitting-position knee-adjustment manipulations can reduce pain and ameliorate joint function in patients with patellofemoral osteoarthritis, producing more significant efficacy than oral celecoxib capsules.
8.Studies on the Acute Toxicity in Mice and Cytotoxicity of the Impurity in Ornithine
Wangping PAN ; Xiaoling XU ; Bei CAI ; Xiaojun LÜ ; Kaiyong HE
China Pharmacist 2018;21(2):346-348
Objective:To study the acute toxicity in mice and cytotoxicity of the impuritiy(3-amino-2-piperidinone hydrochloride) in ornithine. Methods:The mice were given the impurity by intravenous injection.The acute toxicity was observed and LD50in mice was calculated by Bliss method. According to the result of LD50,the mice were given the impurity respectively at high,medium and low dose. The changes of general condition and body weight were recorded,and the blood biochemical indices and histomorphology of organs and tissues were observed after 14 days. The cytotoxicity was measured on fibroblasts(L929) cells. Results:The LD50and 95% con-fidence limit of the impurity (intragastric administration) was 758.49-848.08 mg·kg-1.All the detected indices of the three groups (660,330 and 170 mg·kg-1) including general condition,weight change,biochemical indices,organ morphological and histological change were all within the normal ranges. The impurity significantly decreased the viability of L929 cells. The NOAEL value was 660 mg·kg-1in mice. Conclusion:The impurity has certain toxicity to mice and cells. The data can provide experimental basis for the quality standard establishment for ornithine and the safety improvement in clinic use.
9.Immunotoxicity of absorbable silk fibroin biofilm on rats
Xiaoling XU ; Wangping PAN ; Xiaojun LÜ ; Ju ZHANG ; Yuanhua HU ; Kaiyong HE
Chinese Journal of Tissue Engineering Research 2019;23(14):2190-2195
BACKGROUND: Silk fibroin, the main component of silk fibroin biofilm, is a natural protein, but it is still a heterologous protein to the body. Its immunogenicity/toxicity is an important factor in determining the development prospects. OBJECTIVE: To evaluate the toxicity of absorbable silk fibroin biofilm and its degradation products on the rat immune system by muscle implantation experiments in rats. METHODS: Ten Wistar rats from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. were implanted with a long strip of 1 mm× 10 mm in the right gluteal muscle to observe the implant absorption and determine the implantation cycle. Seventy-two Wistar rats were assigned into control and experimental groups (n=36/group, 18 in either sexes) . A long strip of 1 mm×10 mm was implanted into the rat right gluteal muscle in the experimental group, and the control group received no implantation. Some of the rats were taken for histological examination and calculate the organ/weight ratio, at 26 weeks postoperatively. The spleen lymphocyte proliferation ability, NK cell activity, cell classification of T lymphocytes, and levels of interleukin 2 and tumor necrosis factor α were detected. Another part of the animals was taken for macrophage phagocytosis of erythrocytes cell capacity and antibody producing cell count. RESULTS AND CONCLUSION: (1) Silk fibroin biofilm was completely absorbed after implanted into the rat muscle for 26 weeks. (2) In female or male Wistar rats, the immune organs in the experimental group showed no significant changes in the appearance, weight and histological examination. There were no significant differences in the hematological indexes (hemoglobin, red blood cell count, hematocrit, blood platelet count and white blood cell count and classification) , spleen lymphocyte proliferation, NK cell activity, the ratio of T lymphocytes and their subpopulations, and the levels of interleukin 2 and tumor necrosis factor α had no significant differences between two groups (P> 0.05) . (3) In female or male Wistar rats, the macrophage phagocytosis of chicken erythrocytes cell capacity and antibody producing cell count showed no significant differences between two groups (P> 0.05) . (4) These results indicate that silk fibroin biofilm causes no immunosuppression or immunostimulation on immune organs, immune cells and immune molecules (cytokines) of rats.
10.The past,present,and future of in vivo-implant-able recording microelectrodes:the neural interfaces
Kun LIU ; Hao ZHANG ; Minghui HU ; Zifa LI ; Kaiyong XU ; Dan CHEN ; Wenqiang CUI ; Cui LYU ; Ran DING ; Xiwen GENG ; Sheng WEI
Chinese Journal of Pharmacology and Toxicology 2023;37(7):553-553
Neural recording electrodes enable the acquisition and collection of electrical signals from neu-rons,and these recorded neural electrical signals are an important means of understanding neuronal activity.As a major component of the brain-machine interface,neu-ral recording electrodes serve as a bridge between the nervous system and external devices.The extracted information can be used to understand the state of the brain and acts as a feedback signal to regulate external devices,thus providing important information for the clini-cal treatment of neurological diseases.Moreover,the electrodes can be used as a vehicle for drug injection to directly treat diseases.Since the time that Strumwas-ser used microwires to achieve long-term recordings of neural activity in hibernating squirrels,implantable elec-trode technology has gradually improved over three gen-erations of development,and progress has been made in improving the biocompatibility,mechanical performance(size,shape,density,etc.),and signal-to-noise ratio.Implantable neural recording electrodes can acquire sig-nals from cortical and deep neural clusters,with the advantages of high signal-to-noise ratio,information con-tent,and spatial/temporal resolution.However,there is still a need to improve the structure and performance of these electrodes;for example,their high invasiveness and lack of biocompatibility pose technical difficulties in the process of translation to the clinic.This paper reviews the basic requirements for electrodes,main recording methods and signal types,common types of implant-able neural recording electrodes,and their challenges and future development directions.With the continuous development of electrode materials,equipment,systems,and neurotechnology,it should be possible to apply neu-ral recording electrodes in clinical practice,to promote safe and efficient treatment of human diseases.