At present,the main treatment methods of the patients with advanced colorectal cancer include surgery,chemotherapy,radiotherapy,targeted therapy,physical ablation and immunotherapy,but the chemotherapy is still the main treatment.The emergence of new chemotherapy drugs and the combination of radiotherapy,chemotherapy and targeted therapy in clinical have improved curative effect for the patients with advanced colorectal cancer.In order to better improve the quality of life,reduce side effects and obtain the best effect,now the individual multidisciplinary treatment has become an inevitable trend in the treatment of advanced colorectal cancer in clinical.

Objective To investigate the relationship between the expression of KAI1 mRNA and the clinicopathological parameters and prognostic outcome of patients with gastric Callcer. Methods The expressions of KAI1 mRNA in 70 samples of gastric cancer tissue and 18 samples of benign gastric lesion were detected by in situ hybridization.Results The positive rates of KAI1 mRNA expression in benign gastric lesion and gastric cancer tissue were 94%(17/18)and 31%(22/70),respectively.The expression of KAI1 mRNA was influenced by tumor difierentiation degree and the state of lymph node metastasis.The positive rate of KAI1 mRNA expression was gradually decreased as the increase of invasion depth and the tumor clinical stages.The 5-year survival rate of patients with positive expression of KAI1 mRNA WaS higher than these with negative expression of KAI1 mRNA.Conclmions The less and down-regulation of KAI1 gene expression may be involved in the occurrence and development of gastric cancer.KAI1 gene might be a valuable indicator for early diagnosis and predicting the malignancy and metastasis potential of gastric cancer and the prognosis of patients with gastric cancer.

Objective　To study the relationship between the expression of p16 and nm23-H1 and clinicopathology in the patients with gastric cancer. 　Methods　p16 and nm23-H1 protein in cancer tissue of 65 patients were observed with labelled streptavidin biotin (LSAB) immunohistochemical method.　Results　The total positive rate of p16 and nm23-H1 was 30.8% and 41.5% respectively in gastric cancer tissues. The positive expression rate of p16 was significantly higher in well-differentiated cancer than in poorly-differentiated cancer (P＜0.05). The positive expression rate of p16 and nm23-H1 was higher in patients with lymph node metastasis than in those without lymph node metastasis(P＜0.05，P＜0.01) and in clinical stage Ⅰ,Ⅱ than in stage Ⅲ (P＜0.05，P＜0.01).The 3-year survival rate was higher in positive cases of p16 or nm23-H1 protein expression (45.0%，51.9%）than in negative ones（28.9%、21.1%）(P＜0.05).There was a significants positive correlation between p16 and nm23-H1 protein expression(r=0.041*!5,P＜0.05).　Conclusions　p16 and nm23-H1 may play a role in the development of gastric cancer and be related to partial biological behavior. Expression of p16 and nm23-H1 may serve as a marker for predicting the cancer metastasis and evaluating the prognosis of gastric cancer patients.

Objective　To study the relationship between the expression of p16 and nm23-H1 and clinicopathology in the patients with gastric cancer. 　Methods　p16 and nm23-H1 protein in cancer tissue of 65 patients were observed with labelled streptavidin biotin (LSAB) immunohistochemical method.　Results　The total positive rate of p16 and nm23-H1 was 30.8% and 41.5% respectively in gastric cancer tissues. The positive expression rate of p16 was significantly higher in well-differentiated cancer than in poorly-differentiated cancer (P＜0.05). The positive expression rate of p16 and nm23-H1 was higher in patients with lymph node metastasis than in those without lymph node metastasis(P＜0.05，P＜0.01) and in clinical stage Ⅰ,Ⅱ than in stage Ⅲ (P＜0.05，P＜0.01).The 3-year survival rate was higher in positive cases of p16 or nm23-H1 protein expression (45.0%，51.9%）than in negative ones（28.9%、21.1%）(P＜0.05).There was a significants positive correlation between p16 and nm23-H1 protein expression(r=0.041*!5,P＜0.05).　Conclusions　p16 and nm23-H1 may play a role in the development of gastric cancer and be related to partial biological behavior. Expression of p16 and nm23-H1 may serve as a marker for predicting the cancer metastasis and evaluating the prognosis of gastric cancer patients.

BACKGROUNDThere is no effective regimen for recurrent small cell lung cancer patients now. The aim of this study is to assess the activity and toxicity of topotecan combined with cisplatin in the treatment of recurrent small cell lung cancer patients.

METHODSTwenty-eight patients with progressive disease after one first-line regimen enrolled in the study from May 2002 to October 2004. Topotecan was given at the dose of 1.2mg/m² from 1st to 4th days and cisplatin was administered at the dose of 25mg/m² from 5th to 7th days in a cycle of 3 weeks. The patients could be evaluated at least after 2 cycles.

RESULTSOne patient got complete response and ten patients got partial response in this group. The overall response rate was 39.3% , and the response rate of refractory group and sensitive group was 37.5% (3/8) and 40.0% (8/20) respectively. The median time to progression was 4.2 months. The main toxicity was hematological toxicity. Grade III+IV neutropenia occurred in 42.9% (12/28) of the patients.

CONCLUSIONSThe regimen of topotecan and cisplatin shows better activity in retreated small cell lung cancer patients. The main toxicity is hematological toxicity and can be tolerated.