In this article a new principle of geometric design for blade's surface of an impeller is provided. This is an optimal control problem for the boundary geometric shape of flow and the control variable is the surface of the blade. We give a minimal functional depending on the geometry of the blade's surface and such that the flow's loss achieves minimum. The existence of the solution of the optimal control problem is proved and the Euler-Lagrange equations for the surface of the blade are derived. In addition, under a new curvilinear coordinate system, the flow domain between the two blades becomes a fixed hexahedron, and the surface as a mapping from a bounded domain in R2 into R3, is explicitly appearing in the objective functional. The Navier-Stokes equations, which include the mapping in their coefficients, can be computed by using operator splitting algorithm. Furthermore, derivatives of the solution of Navier-Stokes equations with respect to the mapping satisfy linearized Navier-Stokes equations which can be solved by using operator splitting algorithms too. Hence, a conjugate gradient method can be used to solve the optimal control problem.

In this article a new principle of geometric design for blade's surface of an impeller is provided. This is an optimal control problem for the boundary geometric shape of flow and the control variable is the surface of the blade. We give a minimal functional depending on the geometry of the blade's surface and such that the flow's loss achieves minimum. The existence of the solution of the optimal control problem is proved and the Euler-Lagrange equations for the surface of the blade are derived. In addition, under a new curvilinear coordinate system, the flow domain between the two blades becomes a fixed hexahedron, and the surface as a mapping from a bounded domain in R2 into R3, is explicitly appearing in the objective functional. The Navier-Stokes equations, which include the mapping in their coefficients, can be computed by using operator splitting algorithm. Furthermore, derivatives of the solution of Navier-Stokes equations with respect to the mapping satisfy linearized Navier-Stokes equations which can be solved by using operator splitting algorithms too. Hence, a conjugate gradient method can be used to solve the optimal control problem.

0.05),while the activities of SOD of bone tissue were lower(P

In this article a new principle of geometric design for blade's surface of an impeller is provided. This is an optimal control problem for the boundary geometric shape of flow and the control variable is the surface of the blade. We give a minimal functional depending on the geometry of the blade's surface and such that the flow's loss achieves minimum. The existence of the solution of the optimal control problem is proved and the Euler-Lagrange equations for the surface of the blade are derived. In addition, under a new curvilinear coordinate system, the flow domain between the two blades becomes a fixed hexahedron, and the surface as a mapping from a bounded domain in R2 into R3, is explicitly appearing in the objective functional. The Navier-Stokes equations, which include the mapping in their coefficients, can be computed by using operator splitting algorithm. Furthermore, derivatives of the solution of Navier-Stokes equations with respect to the mapping satisfy linearized Navier-Stokes equations which can be solved by using operator splitting algorithms too. Hence, a conjugate gradient method can be used to solve the optimal control problem.

Objective To construct a recombinant lentiviral expression vector for RNA interference (RNAi) of human Snail gene and to study its effects on the proliferation and invasion of nasopharyngeal carcinoma cell line 5-8F. Methods The effective sequence of short hairpin RNAs (shRNA) targeting Snail gene was designed and cloned into the linear pLVTHM vector after enzyme digestion. After confirmation by DNA sequencing, 5-8F cells were infected with the viral supernatants. The cells with stable Snail gene knock-down were separated by fluorescence activated cell sorter (FASC). The expression of Snail mRNA was detected by real time RT-PCR. MTT and cell invasion assay were used to detect the proliferation and invasion of 5-8F cells after plVTHM-siSnail transfection. Results The lentivirus vector plVTHM-siSnail was constructed successfully. The separated 5-8F-plVTHM-siSnail exhibited significant knock-down of Snail mRNA expression. Slower proliferation and decreased cells to permeate through the Matrigel were found after plVTHM-siSnail transfection (P

AIM: To investigate the expression map of two p53 binding proteins 53BP1 and 53BP2 in nasopharyngeal carcinoma (NPC) tissue.METHODS: The expression of 53BP1 and 53BP2 mRNA in NPC biopsy and control group are tested by RT-PCR. The expression of two mRNA in NPC paraffin section are examined by in situ hybridization. RESULTS: No expression of 53BP1 mRNA was found in NPC tissue and control group. However, expression of 53BP2 was detected in NPC biopsy and control group by RT-PCR, specific expressoin found cancerous nest in NPC paraffin section by in situ hybridization.CONCLUSION: The high expression of 53BP2 may be related to the development of NPC.

AIM: Our study focused on investigation of tissue specific regulatory activity of the newly cloned promoter: PLUNC-p with driving enhanced green fluorescent protein ( EGFP ). METHODS: Transgenic Xenopus Laevis system was applied.RESULTS: The green fluorescence protein directed under PLUNC-p was expressed strictly in branchial arches and epidermis of Xenopus Laevis embryos while CMV promoter showed ubiquitous regulation characteristic.CONCLUSION: PLUNC-p is able to direct epithelia specific expression of EGFP . This property of PLUNC-p might raise the possibility that lead target genes to express tissue-specifically.

AIM:To examine the latent membrane protein 1(LMP1)-DNA sequence in nasopharyngeal carcinoma(NPC) and detect mRNA expression of LMP1, EBNA1, EBNA2, and to explore the relationship between EBV infectious status, expression products and NPC carcinogenesis.METHODS: LMP1 DNA was detected in NPC by PCR. Direct sequence was applied to analyze the difference between NPC-LMP1-DNA and B95-8-LMP1-DNA. mRNA expressions of LMP1, EBNA1, EBNA2 in NPC were detected by nested RT-PCR.RESULTS: LMP1 DNA existed in all 47 NPC tissues. Several single nucleotide variations were found between NPC-LMP1-DNA and B95-8-LMP1-DNA. The notable variation was the lost of XhoⅠrestriction site in NPC. Direct sequence showed 30 bp deletion in NPC. The mRNA expressions of LMP1, EBNA1 and EBNA2 in NPC were 76.6%, 80.0% and 74.5% respectively by nested RT-PCR. The expression of EBNA1 in NPC was promoted by Q promoter while the expression of EBNA1 in B95-8 was promoted by C promoter.CONCLUSION: The way of EBV involved in NPC is complex. Latent genes such as LMP1, EBNA1 and EBNA2 as well as early lytic gene BARF1 may all play certain roles in NPC carcinogenesis.

Objective To screen potential genes associated with drug resistance and multidrug resistance. Methods Microarray with 8000 genes was used to detect the different expression of 5-8F cells and 6-10B cells. Subsquently, genes of drug resistance and multidrug resistance were screened by MILANO online programme. Semiquantitative RT-PCR was utilized to confirmed the reliability of differentially expressed genes. Results 283 genes were identified the differential expression. Of these, 85 genes were shown to be upregulated and 98 downregulated. After the analysis of MILANO, 4 genes including UGT1A9 (15.85 folds),MVP(6.77 folds), CAV1(2.49 folds) and HIF1A(2.67 folds) with higher expression in 5-8F cells were found to be likely associated with drug resistance and multidrug resistance. Subsquently, semiquantitative RT-PCR confirmed the reliability of differential expression of these 4 genes. Conclusion Differentially expressed genes shown in NPC 5-8F cells compared to 6-10B cells with the identification of online MILANO program analysis are likely associated with drug resistance and mnltidrug resistance of NPC cells with the ability of metastasis.

OBJECTIVETo compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.

METHODSAfter hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.

RESULTSOf 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.

CONCLUSIONDNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.

DNA Repair ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Nasopharynx ; metabolism ; pathology ; Oligonucleotide Array Sequence Analysis ; methods ; RNA, Neoplasm ; genetics ; Reverse Transcriptase Polymerase Chain Reaction