0.05) . NNS could decrease blood viscosity and hematocrit, inhibit the aggregation of erythrocytes and platelet and increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH -Px). [Conclusion] NNS has an effect on AIS, which is similar to nimodipine. Its therapeutic mechanism may be related to the relief of cerebral ischemia by improving blood rheology and cerebral blood flow and protecting brain cells from injury by eliminating free radicals.

[Objective] To explore the protective mechanism of Naoxing Nasal Spray (NNS) on ischemic cerebral injury. [Methods] Seventy Wistar rats were randomized into 7 groups: high-dose NNS group (group A), moderate-dose NNS group (group B), low-dose NNS group (group C), nimodipine group (group D), model group (group E), solvent control group (group F) and pseudo-operation group (group G). Rat models of ischemia/reperfusion were established by blocking left middle cerebral artery. Plasma levels of tumor necrosis factor a (TNF-?) and interleukin 1? (IL-1?), malondialdehyde (MDA) content, activities of superoxide dismustase (SOD), glutathione peroxidase (GSH-Px) and nitric oxide synthase (NOS) in brain homogenate were detected. [Results] NNS decreased the contents of MDA, TNF-? and IL-1?, and increased the activities of SOD, GSH-Px and NOS. [Conclusion] NNS has protective effect on ischemic cerebral injury by promoting the clearance of free radicals, inhibiting the release of inflammatory mediator and increasing the synthesis of NO and thus to reduce injury of free radicals, inhibit the local inflammatory reaction and improve cerebral blood perfusion.

To investigate the effects of Bushen Yixin Tablet (BYT) on plasma lipid peroxide (LPO) and sexual hormone in renal hypertension rats. Goldblatt's renal hypertension rat models were established. All rats were allocated to six groups: BYT groups (with high, moderate and low dosage of BYT respectively), captopril group, normal saline (NS) group and mimic-operation group. The treatment course lasted 4 weeks. Blood pressure was lowered in BYT groups (P

ive] To observe the therapeutic effect of Naoxing Granule (NG) for acute ischemic stroke (AIS) and to explore its mechanism. [Methods] Single-blind controlled trial was applied. Two hundred cases of AIS were randomly allocated to Group A (n = 150, treated with NG) and Group B (n = 50, treated with nimodipine). Therapeutic effect of NG was observed and plasma free radical levels and the ratio of thromboxane A2 (TXA2) and prostaglandin 2 (PGI2) were detected. [Results] The total effective rate was 91.3%and 76.0% , and the remarkably effective rate was 62.0% and 46.0% in Group A and Group B respectively ( P

BACKGROUND: Brain-awakening nasal sprayer is composed of many herbs,such as Chuanxiong and Shichangpu, which were regarded by "Shennong Bencaojing" as having the function of "preventing stroke in the brain".OBJECTIVE: To observe the changes of free radicals and nitric oxide synthase in rat brain following focal ischemic-reperfusional injury due to brain-awakening nasal sprayer intervention and compare with that due to classical nimodipine.DESIGN: A randomized controlled study.SETTING: Department of internal medicine of a hospital affiliated to a traditional Chinese medical university.MATERIALS: Seventy adult male Wistar rats of clean grade, were randomly divided into seven groups: brain-awakening nasal sprayer of higher dosage group, moderate dosage group, lower dosage group, nimodipine intraperitoneal injection group, physical saline nasal sprayer group, menstruum nasal sprayer group, and sham operation group with 10 rats in each.METHODS: Focal brain ischemia-reperfusion model was established by blocking the left cerebral middle artery in rats of all the groups except sham operation group. Three days before model establishment and during reperfusion, rats were given different dosages of brain-awakening nasal sprayer composed of Chuanxiongqin and Shichangpu of 5.4, 2.7, 1.08 mg/(kg · d) and 1.35, 0. 54, 0.27 g/(kg· d), respectively, three times a day; which was replaced by physical saline and menstruum nasal sprayer of 0. 18 mL/ (kg · d),three times a day in physical saline nasal sprayer group and menstruum nasal sprayer group; rats in nimodipine intraperitoneal injection group received intraperitoneal injection of nimodipine by 0. 8 mg/(kg · d) twice a day. Rats in sham operation group were routinely raised. The content of prodialdehyde, superoxide dismutase and nitric oxide synthase were measured with colorimetric method.MAIN OUTCOME MEASURES: ① The changes of prodialdehyde content, superoxide dismutase and nitric oxide synthase activity in rat brain following focal ischemic-reperfusional injury in groups of different dosage of brain-awakening nasal sprayer and other groups. ② Comparison between different dosage brain-awakening nasal sprayer intervention groups and nimodipine intraperitoneal injection group.RESULTS: Eight rats died during model establishment and the other 62 rats entered the results analysis. ① Content of prodialdehyde: It was significantly lower in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group [ (0.92 ± 0. 32), (0. 87 ± 0. 39)vs(1.35 ±0. 34) μmol/g, P ＜ 0.05], but there was no difference between higher dosage group and nimodipine intraperitoneal injection group. ② Activity of superoxide dismutase: It was obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (35.64 ± 11.67), (33.88 ± 7. 15) vs(20. 70 ± 3.88) NU/mg,P ＜ 0. 05 ], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group. ③ Activity of nitric oxide synthase and superoxide dismutase: It was found obviously higher in higher dosage group and nimodipine intraperitoneal injection group than in physical saline nasal sprayer group[ (4.64 ± 1.22), (5.00 ± 1.10) vs (3.08 ± 1.12) mkat/g, P ＜ 0.05], but no difference could be observed between higher dosage group and nimodipine intraperitoneal injection group.④ The activity of nitric oxide synthase and superoxide dismutase slightly increased while prodialdehyde slightly decreased in moderate dosage group,lower dosage group and menstruum nasal sprayer group, which did not differ significantly from physical saline nasal sprayer group.CONCLUSION: Brain-awakening nasal sprayer intervention exerts multiple effects such as preventing lipo-peroxidation following brain ischemic- reperfusional injury, in addition to suppressing prodialdehyde production, attenuating injury induced by free radicals and increasing nitric oxide synthase activity, thereby playing a similar role to nimodipine in protecting against brain ischemic-reperfusionaldamage

A total of 186 type 2 diabetic patients(DM group)and 72 healthy subjects(NDM group)were enrolled. Metabolic parameters and serum irisin levels were measured. The effects of intensity and time of the physical activity were evaluated. According to quartiles of plasma irisin levels,all subjects were divided into four groups and the prevalence of type 2 diabetes mellitus(T2DM) among four groups was compared. The relationship between plasma irisin level and physical activity in DM group was investigated. The results showed that plasma irisin levels were correlated negatively with the prevalence of T2DM(OR=0.984,95% CI 0.973~0.996);also negatively correlated with age(r=-0. 227, P=0. 029) and blood uric acid (r=-0. 225, P=0. 032) in the DM group. When corresponding exercise time was up to grade 4,the intensity of exercise would influence the irisin levels(χ2=7.319, P=0.025). After controlling for potential confounders such as age,metabolic parameters,intensity of work,timing of work,plasma irisin levels in DM group were correlated positively with the intensity of exercise (β=0.326, P=0.014) and negatively with corresponding exercise time(β=-0.454,P=0.001). These results suggest that the prevalence of T2DM decreases with the increase of plasma irisin level. Plasma irisin levels are increased after moderate intensity exercise(5~7 times weekly and 1h each time) or by short-term high-intensity exercise.