OBJECTIVE: To investigate the association between transforming growth factor beta1(TGF-beta1)-800G>A polymorphism and cerebral infarction (CI). METHODS: The genotypes of 247 patients with atherosclerotic cerebral infarction and 167 sex- and age-matched healthy controls were detected by PCR-RFLP, and the frequency distribution of alleles calculated. RESULTS: TGF-beta1-800G>A polymorphism was found in Han population in Hunan Province, China. The distribution of -800G>A genotypes was consistent with Hardy-Weinberg balance in the CI and healthy control group. There was no significant difference between genotypes and allele frequency of TGF-beta1-800G>A in the CI and control group (P>0.05). Logistic regression analysis suggested that no genotypes could increase or reduce the risk of CI onset (P>0.05). CONCLUSION TGF-beta1-800G>A polymorphism is not associated with CI in Han population in Hunan Province.
Case-Control Studies ; Cerebral Infarction ; genetics ; China ; ethnology ; Female ; Gene Frequency ; Genotype ; Humans ; Male ; Polymorphism, Genetic ; genetics ; Polymorphism, Single Nucleotide ; Transforming Growth Factor beta1 ; genetics

OBJECTIVETo analyze the relationship between hemomyelogram and sererity of acute appendicitis and identify the best routine blood test feature for perforation.

METHODS721 patients were included in this study, all of whom underwent appendectomy for the clinical diagnosis of appendicitis during the years of 2010-2013. The initial preoperative hemomyelogram was evaluated at different stages of appendicitis. The area under the ROC curve was used to assess the clinical feature with greater diagnostic accuracy of perforation. Total lymphocyte counts of 1.83 was used in the prediction of perforative appendicitis. A group of 467 patients was used for validation to confirm the diagnostic value of the cut-off value.

RESULTSThe percentage of lymphocytes had the closest association with the evolutionary phase of acute appendicitis. Total lymphocyte counts < or=1.83 indicated perforation, with high sensitivity and low specificity.

CONCLUSIONThe percentage of lymphocytes and total lymphocyte counts are helpful as a diagnostic paramete for different stages of acute appendicitis.

Acute Disease ; Appendectomy ; Appendicitis ; diagnosis ; Humans ; Lymphocyte Count ; ROC Curve ; Sensitivity and Specificity