Objective To systematically review the effects of clinical nursing pathways on colorectal cancer patients with colostomy.Methods Studies on the randomized controlled trials (RCT) of clinical nursing pathways on colorectal cancer patients with colostomy were systematically searched in electronic databases including CNKI, VIP, CBM and WANFANG Data. Meta-analysis was performed using RevMan 5. 3 software. Results A total of 16 studies enrolling 1 159 patients (582 in observation group and 577 in control group) were included. The results of the Meta-analysis indicated that patients′satisfaction [RR=1.24, 95 CI%=(1.17,1.32), P<0.01] and self-care ability of patients [RR=1.58, 95 CI%=(1.38,1.82), P<0.01] were significantly higher in observation group than in control group. Average hospitalization time [ SMD=-1. 65, 95 CI%=(-2. 66,-0.65) , P<0.01] , the medical expenses [ SMD=-3.89, 95 CI%=(-6.90,-0.87) , P<0.01] and postoperative complications [RR=0.39, 95 CI%=(0.27,0.55), P<0.01] in observation group were significantly decreased while comparing to control group. The descriptive analysis showed that the mastering of health knowledge in the clinical nursing pathway group were better than those in control group.Conclusions Application effects of clinical nursing pathways are superior to the traditional nursing methods in colorectal cancer patients with colostomy.

Objective To systematically evaluate the effect of ostomy bag on drainage tube orifice seepage after abdominal operation.Methods The databases such as the Cochrane Library,PubMed,Embase, CNKI,VIP database,SinoMed (CBM) and WANFANG database were searched for randomized control trials on the application of ostomy bag on drainage tube seepage after abdominal operation,and the retrieval time was limited from database establishment to 31st November,2016. The meta-analysis was performed by the RevMan 5.3 software.Results Finally 12 articles with a total 627 patients met the inclusion criteria,including 316 patients in the observation group and 311 patients in the control group. The Meta-analysis results showed that using ostomy bag to deal with tube seepage could significantly reduce the average cost [SMD=-5.42,95％CI (-6.32,-4.53),P<0.01] and times of daily dressing change[SMD=-2.78,95％CI (-3.22,-2.34),P<0.01], shorten the healing time of sinus [SMD=-1.63,95％CI (-1.86,-1.40),P<0.01],reduce the incidence of irritant dermatitis [RR=0.13,95％CI (0.06,0.26),P<0.01]. The differences were statistically significant. Conclusions Application of ostomy bag to deal with drainage tube seepageis significantly superior to the traditional nursing method.

Objective: To compare the carcinogenic abilities of CD133⁺CD44⁺ laryngeal cancer stem cells and general laryngeal cancer stem cells and to identify the mechanism underlying the action of miRNAs. Methods: Solid tumor-derived laryngeal carcinoma stem cells and Hep-2-derived laryngeal carcinoma stem cells were cultured, and CD133⁺CD44⁺ laryngeal cancer stem cells were sorted by flow cytometry. Boden chamber invasion assay, cell migration assay and tumor formation assay were then performed to compare the invasion, migration and tumorigenic abilities of CD133⁺CD44⁺ laryngeal cancer stem cells and general laryngeal cancer stem cells. And then, miRNAs isolated from two laryngeal cancer stem cells were detected and analysed with miRNA chip. Results: (1)In Boyden chamber invasion assay, the cell invasion rate of CD133⁺CD44⁺ laryngeal cancer stem cells was obviously higher (80.2%±2.3% vs. 63.9%±3.2%, t=5.011, P=0.027); (2)CD133⁺CD44⁺ laryngeal cancer stem cells also had higher mobility in cell migration assay (82.9%±1.1% vs. 70.9%±0.6%, t=4.514, P=0.031); (3)In tumor formation assay, the tumor formation rate of CD133⁺CD44⁺ laryngeal cancer stem cells was also higher (80% vs. 50%). What′s more, we identified 15 miRNAs that were significantly upregulated in CD133⁺CD44⁺ laryngeal cancer stem cells and 3 miRNAs that were significantly downregulated in CD133⁺CD44⁺ laryngeal cancer stem cells, compared with normal laryngeal cancer stem cells. Conclusions CD133⁺CD44⁺ laryngeal cancer stem cells have stronger invasion, migration and tumorigenic abilities compared with normal laryngeal cancer stem cells, and the difference of miRNAs′ expression is one of the possible causes.