Objective To analyse distribution and antibacterial resistance of common pathogenic bacteria of clinical infectious diseases and provide scientific basis for the treatment of infectious diseases ,rational use of antibacterial agents and nosocomial infec‐tion control .Methods The distribution and antibacterial resistance of common pathogens clinically isolated from inpatients and out‐patients from August 2012 to July 2013 were analyzed .Bacterial identification and drug susceptibility test were carried out by using MicroScan WorkAway 40 automated bacterial identification and drug‐susceptibility analyzer ,and the results of drug susceptibility test were analysed by using the WHONET5 .5 software .Results A total of 1 434 strains of pathogenic bacteria were isolated ,and gram‐negative bacteria ,gram‐positive bacteria and other pathogenic bacteria accounted for 53 .8% ,28 .1% and 18 .1% respectively . The detection rate of methicillin‐resistant Staphylococcus aureus and methicillin‐resistant coagulase negative Staphylococcus were 31 .6% and 57 .4% respectively .No strains of vancomycin‐resistant Staphylococcus were found ,while strains of vancomycin‐resist‐ant Enterococcus faecium accounted for 8 .8% .The detection rate of extended‐spectrum beta‐lactamases producing Escherichia coli and Klebsiella pneumoniae were 47 .2% and 12 .2% respectively .Isolates of Staphylococcus and Enterococcus faecium were sensi‐tive to vancomycin ,linezolid and moxifloxacin ,and the resistance rates were less than 10 .0% .While isolates of Staphylococcus were resistant to the penicillin ,macrolides ,quinolones ,tetracycline ,cephalosporin ,and the resistance rates were more than 30 .0% .Most isolates of gram‐negative bacteria were sensitive to ertapenem ,imipenem ,amikacin ,meropenem ,piperacillin/tazobactam ,ticarcillin/clavulanic acid and levofloxacin ,and the resistance rates were less than 10 .0% .Conclusion It is necessary to enhance monitoring of distribution and antibacterial resistance of pathogenic bacteria ,so as to provide references for guiding rational use of antibacterial a‐gents in different departments .