Objective To investigate the clinical value of serum follicle stimulating hormone(FSH) and testicular volume in the etiology diagnosis of azoospermia.Methods The clinical data of 373 patients with azoospermia were analyzed retrospectively.The patients were divided into the group A,B and C according to their FSH detected level.The FSH level was normal in the group A,slightly increase in the group B and severe increase in the group C.The testicular volume and the spern retrieval success rate of each group were analyzed statistically.Results The testicular sperm retrieval success rates in the group A,B and C were 70.81％,22.22％ and 0 respectively;the average testicular volumes in the three groups were(11.39±5.06),(8.79±4.18),(6.96±4.12)mL respectively,the differences among the three groups were statistically significant(P＜0.05).Conclusion In the patients with azoospermia,the FSH level and testicular volume help to judge the testicular spermatogenic functional status and identify the type of azoospermia.