Objective To explore the effect of xanthine oxidase inhibitors allopurinol on collagen remodeling of non-infarcted myocardium after myocardial infarction in rats.Methods Myocardial infarction models were produced by ligation of anterior descending coronary artery.The survivals were randomly divided into 3 groups:sham operation group, MI group and allopurinol group(50 mg/kg).On the 7th, 14th, 21st and 28th day respectively, the collagen content were examined. The type Ⅰ and type Ⅲ collagen volume fraction(CVF) and Ⅰ/Ⅲ ratio in non-infarcted zones(NIZ) were examined with PSR staining, mRNA expressions were detected with RT-PCR, the activities of xanthine oxidase(XO) and O—?2,?OH-scavenging in NIZ were examined by colorimetry.In addition, the expression of XO protein by Western blotting analysis and pathological change in LV were detected on the 28th day.ResultsCompared with sham group,the mRNA expressions, CVF of typeⅠand type Ⅲ collagen in NIZ were increased in MI group.The typeⅠ/Ⅲ ratio in NIZ was increased after decreased.The collagen content and activity of XO were boosted but the activities of O—?2,?OH-scavenging were decreased(P

Objective To explore the effect of xanthine oxidase inhibitor allopurinol on cardiomyocyte apoptosis in rats after myocardial infarction(MI).Methods MI model was established by the ligation of anterior descending coronary artery.The survivors were randomly divided into 3 groups: sham operation group(n=5),MI group(n=16) and allopurinol group(n=15,receiving allopurinol 50 mg?kg-1? d-1).After 28 days,the infarct size was measured.In non-infarcted zone(NIZ),cardiomyocyte apoptosis was detected by TUNEL;the expression of Fas was detected by immunohistochemistry;the expressions of xanthine oxidase(XO) and caspase 3 were detected by Western blot.In addition,the activities of XO and ?O-2,?OH-scavenging in NIZ were detected by colorimetry.Results Compared with sham operation group,the apoptosis index and expressions of Fas,XO,caspase 3 in NIZ were significantly increased in MI group.The activity of XO was increased but the activities of ?O-2 and ?OH-scavenging were decreased(P0.05).Conclusion Allopurinol could inhibit the cardiomyocyte apoptosis in NIZ in rats.The protective mechanism of allopurinol involves the reduction of reactive oxygen species and depression of the expressions of Fas and caspase 3.

OBJECTIVETo evaluate the effect of linearly polarized near-infrared irradiation (Super Lizer) combined with aerothermotherapy in the treatment of patients with frozen shoulder and diabetes.

METHODSEighty-four diabetic patients with frozen shoulder were randomized into 3 groups to receive a 20-day treatment with drug therapy and shoulder exercise (control group), daily linearly polarized near-infrared irradiation in addition to the control therapy (Super Lizer group), or daily Super lizer and aerothermotherapy (S+P group) in addition to the control therapy. Visual analogue scale was used to assess the changes in the pain intensity, and the shoulder movement was assessed using shoulder movement disorder degree integral scores.

RESULTSAfter the 20-day treatment, the VAS scores and shoulder movement disorder degree integral scores in the 3 groups all significantly decreased (P<0.05), and these scores in Super Lizer group and S+P group were significantly lower than those in the control group (P<0.05). The cure rate in S+P group was significantly higher than that in the other two groups (P<0.01).

CONCLUSIONLinearly polarized near-infrared irradiation combined with aerothermotherapy is effective for treatment of patients with frozen shoulder and diabetes with a high cure rate and short treatment course.

Bursitis ; complications ; therapy ; Diabetes Complications ; therapy ; Diathermy ; Female ; Humans ; Infrared Rays ; therapeutic use ; Laser Therapy ; Male ; Middle Aged ; Treatment Outcome