Objective To observe the intracranial tumorigenicity situation of lung cancer cell strain and ovarian cell strain by injecting them into the female nude mice with different approaches and to analyze the possible mechanism for scarce brain metastasis of ovarian cancer to provide a certain thinking for the treatment of brain metastasis of lung cancer.Methods Ovarian cancer cell strain Skov3 and lung cancer cell strain A549 were injected into female nude mice respectively through tail veins,abdominal cavity,common carotid artery and brain tissue.There were 16 nude mice in each approach.All the nude mice were killed 4-6 weeks later.Then the brain,lungs,kidneys,spleen,liver,fallopian tube,ovary and abdominal tumor mass were taken by HE staining for observing the cerebral tumorigenicity situation.Results By the caudal vein injection,there was no brain metastasis in the Skov3 strain group,while in the A549 strain group,2 cases of brain metastasis occurred.By the aabdominal cavity injection,there was no brain metastasis in the Skov3 strain group,but 2 cases of brain metastasis occurred in the A549 strain group.By the common ca rotid artery injection,there was no brain metastasis in the Skov3 strain group,but 8 cases of brain metastases occurred in the A549 group.The intracranial tumorigenicity of Skov3 strain and A549 strain had statistical difference among these 3 kinds of extracranial injection approaches(P＜0.01).By intracranial direct injection approach,14 cases of brain tumorigenicity occurred in the Skov3 strain group,while 10 cases of brain tumorigenicity occurred in the A549 strain group,the difference had no statistical difference.Conclusion Blood brain barrier maybe plays an important role in preventing ovarian cancer cells from metastasizing into brain.

Objective To systematically evaluate the effectiveness and safety of Gegen-Qinlian decoction in treating ulcerative colitis.Methods We searched Cochrane Library, PubMed, CNKI, VIP, CBM and Wanfang online Data bases June, 2017. All of the randomized controlled trials(RCTs) of Gegen-Qinlian decoction compared with sulfasalazine in treating for ulcerative colitis were searched. The quality of RCTs meeting inclusion criteria was evaluated and the data were extracted; meta-analyses were performed with RevMan 5.3 software, and then the GRADE system was used to rate the level of evidence and strength of recommendation.Results Totally 5 RCTs were included into the study. The group treated withGegen-Qinlian decoction or combined with sulfasalazine was superior to the control group in total effective rate (RR=1.18, 95％ CI(1.06-1.30),P=0.002). There were no significant differences between the two groups in clinical symptom curative effect [RR=1.09, 95％CI (0.72-1.64), P=0.69], adverse reactions [RR=0.11, 95％ CI (0.01-1.92),P=0.13], Symptom curative effect [RR=1.33, 95％CI (0.95-1.88),P=0.10], mucosal lesions curative effect [RR=1.33, 95％CI(0.95-1.88), P=0.10]. Based on the GRADE system, the level of total effective rate and adverse reactions evidence was Grade C, and the strength of recommendation was 2. the level of the rest of the evidence was Grade D.Conclusions Compared with sulfasalazine,Gegen-Qinliandecoction or combined with sulfasalazine can be used as a treatment option.