OBJECTIVE To study the pathogenicity of the Vibrio fluvialis isolated from the coastal seawater.METHODS Virulence experiment group:22 Kunming mice were divided into four subgroups in random:V.fluvialis was injected into abdominal cavity in the test subgroup.And Staphylococcus aureus and Escherichia coli were injected into the positive control subgroups,separately and aseptic physiological saline was injected into the negative control group.Wound infection group:22 SPF mice were divided into four supgroups in random after their legs were injured:the experimental supgroup(soaked in artificial seawater with V.fluvialis);the positive control groups(with S.aureus and E.coli,separately);the negative control group(soaked in aseptic artificial seawater).The general condition,blood routine,blood culture,organ culture and wound secretion culture of the mice were observed.The pathological analysis of the mice was taken after sacrifice on the 3rd day.RESULTS In virulence experiment group,among all the 7 mice′s blood culture of V.fluvialis supgroup,5 mice were found V.fluvialis positive after 12 h injection,and 2 mice kept on positive until 24 h.In wound infection group,pathological examination showed there were a large number of neutrophils distributed over the striated muscle of the injured sites and cellulitis formed.CONCLUSIONS The V.fluvialis isolated from the sea water has pathogenicity,and can cause wound) infection and septicemia when the concentration reached 106 CFU/ml.

Objective: To observe the clinical effects of intranasal excision on nasal vestibular cyst under nasal endoscopy. Methods: Forty-two cases of nasal vestibular cyst diagnosed in the Department of Otorhinolaryngology Head and Neck Surgery, Tianjin Third Central Hospital between Feb. 2011 and Jan. 2016 were treated by intranasal excision under nasal endoscope. Results: All the 42 patients were cured without any complication. The rate of complete stripping was 78.6% (33/42), with operating time of (21.31±4.04) min and bleeding amount of (10.26±2.13) ml. During follow-up ranged from 6 months to 5 years, with the median follow-up time being 19.6 months, no post-operative recurrence and complication were found. Conclusion Intranasal excision for nasal vestibular cyst under nasal endoscopy is an effective method, which can be widely used in hospitals.

Objective:To investigate the feasibility and safety of ultrasound-guided percutaneous submandibular region puncture and drainage(PSPD) for treating parapharyngeal space abscess (PPSA).Methods:The clinical data of 26 patients with PPSA receiving PSPD from January 2015 to December 2019 in the Third Central Hospital of Tianjin were retrospectively analyzed.Results:All 26 patients successfully underwent puncture and catheterization with a primary success rate of 100%. After catherization, pain relieved within 12-24 hours, body temperature returned to normal within 24-48 hours, white blood cell(WBC) and C-reactive protein(CRP) returned to normal within 48-96 hours. The catheterization duration ranged from 5 to 14 days and the average time was 7 days. There were statistically significant differences in the body temperature, maximum abscess diameter, WBC and CRP between pre-operation and 7 days after operation(all P<0.001). None of the 26 patients experienced any serious complications such as major vessels, nerve or glands injury. Bacterial cultures were positive in 18 patients and the most common bacteria was hemolytic streptococcus. Conclusions:PSPD is an effective, safe and minimally invasive method for the treatment of PPSA as an alternative to operative incision and drainage.