BACKGROUND:Methods after mechanical stimulation can be combined with various kinds of cells to produce many conversion types so as to start the various physiological functions of the body. OBJECTIVE:To analysis the reason why rol ing massage can improve the intracellular concentration of Ca2+METHODS:A biodynamic experimental device was set up, composed of pressure generating device, pressure forming device, pressure display device and related data recording device. We detected the effects of rol ing massage on Ca . 2+concentration in normal skeletal muscle cells and damaged cells through the different functions of the experimental device and system software. These functions consisted of pressure display, data transmission and recording, signal acquisition and processing, pressure signal acquisition, control and processing of signal acquisition, wireless signal transmission and receiving system, display system, storage system, system implementation function. RESULTS AND CONCLUSION:The experiment results showed that rol ing massage could reduce the Ca 2+concentration in the normal skeletal muscle cells and damaged cells. These findings suggest that rol ing massage can improve the overload of intracellular Ca2+in damaged cells, and it is superior to the sinusoidal mechanical stimulus.

BACKGROUND: Olfactory ensheathing cell transplantation is a better theray for spinal cord injury, and it becomes one of the most promising treatment methods. Local transplantation is applied currently, with the disadvantages of complex operation, large trauma, repeated transplantation. Looking for a simple and effective way for cell transplantation becomes a hotspot for scholars from various countries.OBJECTIVE: To investigate the effect and possibility of transplantation of olfactory ensheathing cells for treatment of spinal cord injury.METHODS: Wistar rats with T 10 spinal cord hemisecti on were divided into 4 groups: intramedullary local transplantation group (A),vein transplantation group (B), D/F12 transplantation group (C) and control group (D). The functional recovery of rats with spinal cord injury was observed with combined behavioral score at different phases. The tissue sections of each group were made at 5 and 10 weeks postoperatively to observe the axon regeneration and the survival of olfactory ensheathing cells.RESULTS AND CONCLUSION: The experiment showed that the rats transplanted with OECs at injured site and through the vein had more improvement in functional recovery and histological changes than the other two groups. The effect between A group and B group had not significant difference. The method of treating spinal cord injury by transplanting OECs via the vein not only simplifies the operation and avoids many complications but also has good curative effect similar to local transplantation.

BACKGROUND: A proper preservation method would be of important significance for experiments and clinical application ofolfactory ensheathing cells (OECs) OBJECTIVE: To explore proper cyropreservative systems for OECs.METHODS: OECs during the logarithmic growth phase were harvested, cryopreserved for 1, 3 and 6 months and then revitalized.RESULTS AND CONCLUSION: MTT assay and tryplan blue staining showed that cells exhibited highest viability after treatmentwith 5% dimethyl sulfoxide (DMSO)-6% hydroxyethyl starch (HES), followed by 10% DMSO, and lastly the 5% DMSO. Use ofrefrigerator or cryogenic control system with different cryopreservation time did not yield obvious effects on viability of OECs.Therefore, 5% DMSO-6%HES is recommended as a cryopreservative agent for OECs.

Viruses are closely related to the occurrence of human diseases, and the infection of some viruses will lead to the occurrence and development of tumors, such as EB virus, hepatitis B virus, HPV virus, etc. On the contrary, some viruses can also be used for the treatment of tumors, such as oncolytic viruses. Lung cancer is a malignant tumor with the highest incidence rate and mortality rate in the world. In recent years, the relationship between virus and lung cancer has attracted more and more attention. Therefore, this article will review the mechanism and therapeutic impact of viruses on lung cancer.

Objective To establish the methodology of combining BOLD and 1H-MRS for investigating correlation between the deactivation in medial prefrontal cortex (MPFC) and gamma-aminobutyric acid (GABA) concentration by acupuncture at LI4 (Point Hegu),and to optimize the experimental technique and procedure.Methods Twenty healthy adult volunteers were enrolled.During fMRI-BOLD scanning,each subject received acupuncture at right LI4 (Point Hegu).MRS scanning was based on MEGA-PRESS sequence,and ROIs were located at bilateral MPFC.The task BOLD fMRI was block design,including 3 stimulations (30 s) with 2 intervals (2 min).Then MRS scanning was performed before and after BOLD.The quantitative values of the BOLD positive and negative activations (Pm) and GABA concentrations were calculated.Results All 20 subjects completed BOLD fMRI scanning,and met the postprocessing requirements.MRS images of 9 subjects with good image quality were included in analysis.Among all 20 subjects,positive activation (Pm=1.17± 0.16) was observed in 9,while negative activation (Pm =-1.31 ± 0.17) was observed in 11 subjects.The GABA average values before and after the acupuncture were (19.93 ±1.04) nmol/L and (20.04±0.81)nmol/L,respectively,and the average amplitude between post-and pre-acupuncture was (0.11 ± 1.60)nmol/L.Conclusion The success rate of this method for quantitative study of brain function established multimodal-functional (BOLD-fMRI and MRS) was acceptable,and the multimodal brain function changes as well as the quantitative values were observed in the brain region during acupuncture.Combined BOLD and MRS quantitative method is feasible for testing acupuncture response in the brain.

Objective:To investigate the effect of cancer nodules on postoperative liver metastasis in patients undergoing radical resection of colorectal cancer.Methods:The clinicopathological data of 196 patients undergoing radical operation for colorectal cancer admitted to the Dongnan Hospital of Xiamen University from January 2019 to June 2020 were retrospectively analyzed. According to the pathological results, they were divided into cancer nodule group and non-cancer nodule group. Time and the number of liver metastasis during the follow-up period were analyzed. The relationship between cancer nodules and clinicopathological features was analyzed, and the influencing factors of postoperative liver metastasis in patients with colorectal cancer were analyzed by univariate and logistic multivaiate analysis. The receiver operator characteristic (ROC) curve was drawn to analyze the predictive effect of cancer nodule diameter on postoperative liver metastasis of colorectal cancer.Results:There were 50 patients accompanied by cancer nodules in 196 patients with colorectal cancer, the incidence rate was 25.5%, the diameter of cancer nodules was 0.4-1.8 cm, the median diameter was 1.0 cm, and there were 46 patients with liver metastasis, the incidence rate was 23.5%. There were statistically significant differences in carbohydrate antigen CA19-9 ( χ2=7.55, P=0.006), maximum tumor diameter ( χ2=12.23, P<0.001), T stage ( χ2=15.79, P<0.001), vascular thrombus ( χ2=12.79, P<0.001), lymph node metastasis ( χ2=16.18, P<0.001) between the cancer nodule group and the non-cancer nodule group. Univariate analysis showed that CA19-9 ( χ2=7.40, P=0.007), maximum tumor diameter ( χ2=8.23, P=0.004), T stage ( χ2=4.58, P=0.032), vascular cancer thrombus ( χ2=12.97, P<0.001), lymph node metastasis ( χ2=3.96, P=0.047), cancer nodules ( χ2=67.60, P<0.001), cancer nodule diameter ( t=4.28, P<0.001), and the number of cancer nodules ( χ2=68.77, P<0.001) were all influential factors for postoperative liver metastasis in colorectal cancer patients. Multivariate analysis showed that stage T 3-4 ( OR=4.62, 95% CI: 1.51-7.35, P=0.003), vascular cancer thrombus ( OR=5.42, 95% CI: 1.75-12.85, P<0.001), accompanied by cancer nodules ( OR=18.54, 95% CI: 7.45-39.73, P<0.001), the diameter of cancer nodules ( OR=13.20, 95% CI: 4.74-29.64, P<0.001), number of nodules [one cancer nodule ( OR=9.11, 95% CI: 2.24-27.34, P<0.001), two cancer nodules ( OR=6.36, 95% CI: 1.38-9.08, P<0.001) and three cancer nodules ( OR=5.00, 95% CI: 1.98-8.84, P<0.001) ] were independent influencing factors for postoperative liver metastasis in patients with colorectal cancer. ROC curve analysis showed that the best threshold of the diameter of cancer nodules for predicting liver metastasis was 1.1 cm [area under the curve (AUC) =0.764, 95% CI: 0.632-0.896, P=0.002). The incidence of liver metastasis in patients with cancer nodules diameter≥1.1 cm was 95.65% (22/23), higher than that in patients with cancer nodules diameter<1.1 cm (40.74%, 11/27), with a statistically significant difference ( χ2=16.69, P<0.001) . Conclusion:Patients with colorectal cancer with cancer nodules≥1.1 cm in diameter should be vigilant against postoperative liver metastasis.