To investigate the monitoring of acute rejection of porcine pancreaticoduodenal allografts, 12 pigs were subjected to pancreaticodudenal allografts, among them 6 pigs received triple therapy for 7 days. Serial biopsies were performed in pancreas allografts for diagnosis of pancreatic acute rejection. Amylase secretion was determined periodically.The levels of TNF ? and NO in serum were serially monitored.The results showed that amylase secretion decreased,TNF ? and NO concentration in serum increased significantly as acute rejection was detected histo pathologically in pancreatic grafts. It suggested that monitoring of amylase secretion, serum TNF ? and NO concentration could be used to decide the proper time of performing biopsy for early diagnosis of acute rejection of the pancreas allograft.

Objective To study the results of laparotomy and Cool-tip radiofrequency ablation to treat large liver tumors.Methods Laparotomy and Cool-tip radiofrequency ablation were carried out on 64 patients with large hepatic cancer.To destroy the tumor completely,for tumors of 3.0～4.0 cm in diameter,7 ablations were required; for 4.0～5.0 cm in diameter 15 ablations; for 5.0～6.0 cm in diameter 19 ablations; for 6.0～7.0 cm in diameter 40 ablations.Result The complete necrosis rate of laparotomy and radiofrequency ablation was 93.75％ (60/64).The short-term results were good.Conclusions Laparotomy and Cool-tip multipoint overlapping radiofrequency ablation for large liver tumors (tumor diameter＞3 cm) could result in a high complete necrosis rate and a low complication rate.It is a good radical treatment for unresectable and large liver cancer.

ObjectiveTo improve the teaching quality of medical humanity education in medical university.MethodsA cluster sampling survey was given to 448 sophomores who studied the outline of medical humanities course in military medical university,and the data were descriptively and statistically analyzed.ResultsThe survey shows that 90％ sophomores satisfy with this course,95％ sophomores understand the importance of medical humanity education,and the students have more requirements on education resources and teaching ways.ConclusionOutline of medical humanities course is important to cultivate the medical humanity spirit of medical students,and the course still needs more improvements.

Objective To investigate the effect of nano-?-linolenic acid on the expression of cathepsin B(CB) in mice with viral myocarditis(VM).Methods Eighty male Balb/c mice were randomly divided into 4 groups:control group,myocarditis group,low-dose intervention group and high-dose intervention group,each group had 20 mice.Mice in control group were inoculated introperitoneally with eagle′s solution,every mouse in the last 3 groups was treated with 0.1 mL Coxsackie B3 virus(CVB3) intraperitoneally.Then,mice in low-dose intervention group and high-dose intervention group were treated with 60 mg?kg-1and 180 mg?kg-1 nano-?-linolenic acid solution for 7 days,respectively.Mice in control group and myocarditis group were treated with 9 g?L-1 saline for 7 days.All mice were killed on the 15th day,and the specimens of hearts and serum were conserved.Myocardial histopathology was determined with hematoxylin and eosin stain.The expression levels of myocardial CB mRNA were detected by reverse transcription polymerase chain reaction.Serum CB concentration was examined by enzyme-linked immunosorbent assay.Results The mortality rate was 0,45%,30% and 20% in control group,myocarditis group,low-dose intervention group and high-dose intervention group,respectively;the mortality rate was significantly lower in high-dose intervention group compared with myocarditis group(P0.05).The expression level of CB mRNA and serum CB concentration were markedly higher in myocarditis group than those in control group(Pa

Objective To evaluate the radiation dose and image quality of MinDose techniques in dual-source CT coronary artery angiography.Methods 120 consecutive patients undergoing cardiac CT scans were randomly assigned into 2 groups: Group A1 with the tube current reduced to 20% of the normal tube current outside the pulsing window,and Group A2 with the tube current reduced to 4% of the normal tube current outside the pulsing window (MinDose).The image quality,noise,volume CT dose index (CTDIvol),and effective dose (E) of these two groups were evaluated.Results The mean score of imaging quality of Group A1 was (4.3 ± 0.3 ),not significantly different from that of Group A2 [(4.5 ±0.4),t=0.16,P＞0.05].The value of CTDIvol of Group A1 was (40.2 ±9.6) mGy,significantly higher than that of Group A2 [( 36.4 ± 9.1 ) mGy,t = 3.2,P ＜ 0.05].The E value of Group A1 was (9.1± 2.2) mSv ,significantly higher than that of A2 [( 8.1 ± 1.9) mSv,t = 2.7,P ＜ 0.05].Conclusion Application of the MinDose technique not only reduces the radiation dose to the patient,but also meets the requirement of diagnosis.

Objective To observe the characteristics and treatment of malignant peripheral nerve sheath tumor originated from neurofibromatosis and the outcome of the patients. Methods A case was reported and the related literatures were reviewed. Results and Conclusion Malignant peripheral nerve sheath tumor is a rare soft tissue malignancy, which is with highly invasion. There are some difficulties with the diagnosis and treatment, and the outcome is poor. Early diagnosis is very important for treatment.

OBJECTIVE: To investigate the characteristics and influencing factors leading to sudden cardiac death (SCD) of incarcerated prisoners. METHODS: Seventy-five SCD cases of prisoners between 2000 and 2013 in Henan province were collected, and environment, psychological and physical factors were retrospectively analyzed. Combined with histopathological results, specific factors of SCD were also studied. RESULTS: In the 75 cases, 21 cases (28%) had definite chronic past medical histories, and 75 cases (100%) had cardiovascular disease confirmed by autopsy. CONCLUSION Due to presence of the potential cardiac diseases, special incarcerated environment, psychological stress, and body-restraint might be the precipitating factors in SCD of those prisoners.
Autopsy ; Cardiovascular Diseases ; Death, Sudden, Cardiac ; Environment ; Humans ; Mental Disorders ; Prisoners ; Retrospective Studies ; Stress, Psychological

BACKGROUND:There are many studies concerning rat bone marrow mesenchymal stem cells for immune tolerance following transplantation and tissue repair.However,there are no reports on umbilical cord mesenchymal stem cells(UCMSCs).OBJECTIVE:To establish a method of separating mesenchymal stem cells(MSCs)from rat umbilical cord,and to study its biological characteristics.METHODS:MSCs were separated from rat umbilical cord with enzyme method and tissue mass method,and then incubated in DMEM-LG medium.Cell morphology was observed under an inverted microscope.Growth curves of cells were drawn using cell counting.Cell cycle and surface antigen were detected with flow cytometry.Adipogenic differentiation and osteogenic differentiation were tested by immunohistochemistry.RESULTS AND CONCLUSION:Both of the two methods could obtain plenty of MSCs from rat umbilical cord.Primary culture showed that the efficiency of enzyme method was higher than tissue mass method.Passage time of the former was about 10 days and the latter was 14 days.The passage time of latter except primary culture was the same.Immunophenotype analysis showed that MSCs from rat umbilical cord expressed adhesion molecule and stromal cell markers,CD90 and CD106,but did not express hematopoietic cell markers,CD34 and CD45.In vitro induction test verified that rat UCMSCs have the potentials of adipogenic and osteogenic differentiation.

Objective To evaluate the clinical effect of the transurethral resection of the prostate (TURP) reserving anterior lobe of the prostate .Methods A total of 736 cases of benign prostatic hyperplasia (BPH ) patients from Guizhou Aerospace Hospital were averagely randomly divided into the observation group and control group .Control group was treated with traditional TURP with the anterior lobe reseated ;while observation group was treated with the similar method to the control group ,but retained the anterior lobe .The clinical therapeutic effects and postoperative complications were observed .Results The maximum flow rate ,re-sidual urine volume and the international prostate symptom score in observation group did not show any statistical significance com -pared with that in control group after surgery (P> 0 .05) .The prevalence rate of temporary incontinence in observation group (1 .09% ) was lower than that in control group(5 .16% ) ,with significant difference(P<0 .05) .The prevalence rate of permanent incontinence in observation group(0 .27% ) was similar to that in control the group(0 .54% ) ,with no significant difference(P>0 .05) .Conclusion TURP reserving the anterior lobe can effectively reduce the incidence of temporary urinary incontinence ,there-fore ,this method could be spread in the clinical practice .

Background Using tablet computer at a short distance is one of the risk factors resulting to pathogenesis and progression of myopia.Studying the effect of such device on accommodative system of human eyes can provide further insights into myopia control.Objective This study was to explore the influences of playing iPAD game for a short duration on accommodative response,accommodative microfluctuation and pupil diameter in adolescent myopia patients.Methods Sixty adolescent myopic patients were included in Peking University People 's Hospital from August 2014 to April 2015,with the mean age (11.6±2.6) years and mean spherical equivalent refraction (SER) (-2.38±1.08)D.The patients were randomly divided into 3 groups according to different playing duration of iPAD game,including 3-minute group,5-minute group and 10-minute group.An open-field infrared refractometer was used to record pupil diameter and accommodation-associated data before and after playing iPAD game.Accommodative response was defined as the difference between the recorded value and theoretical accommodative response,and root mean square (RMS) was used to describe the amplitude of accommodative microfluctuation.One-dimension discrete Fourier transformation was used to analyze spectral characteristic of accommodative response curve.Paired t tests were used to compare these accommodative parameters before and after playing game,while Wilcoxon signed-rank tests were applied for analyzing high frequency component of accommodative microfluctuation.This research conforms to the declaration of Helsinki,and was approved by Peking University People's Hospital Ethics Committee,all the subjects and their guardian signed informed consent.Results The accommodative response values before and after playing iPAD game in 10-minute group were (0.81 ±0.29)D and (0.74 ± 0.27) D,respectively,with significant difference between them (t =2.263,P =0.036),and no significant differences were found in the accommodative response values before and after playing iPAD game in the 3-minute group and 5-minute group (both at P＞0.05).The RMS after playing iPAD game was (0.31 ±0.08) D in the 10-minute group,which was significantly higher than (0.27 ± 0.09) D before playing iPAD game (t =-2.259,P =0.036).The high-frequency spectral power of accommodative response curves in all the three groups were increased after playing iPAD game in comparison with before playing iPAD game,with significant differences in the 5-minute group and 10-minute group (Z =-2.213,-2.016;both at P ＜ 0.05),and the pupil diameters were significantly decreased after playing iPAD game in comparison with before playing iPAD game,with significant differences in the 5-minute group and 10-minute group (t =2.428,P =0.026;t =2.515,P =0.021).Conclusions Short-term exposure to iPAD does not deteriorate accommodative lag in adolescent myopic patients.However,the increase of amplitude and high-frequency spectral power of accommodative microfluctuation,together with accommodation lag will result in blurred images and may bring adverse effects on myopic eyes.