The effects of two lipids lowering drugs, Xu Zhiping and Mixtura Dai Huai, on the LDL metabolism by receptor dependent and independent pathways in experimental hypercholesterolemia rats were obsvered using radioisotopic double-labelling trace technique. The results showed that in the receptor dependent or independent pathways, the FCR of LDL was increased by 43% and 34% respectively in Xu Zhiping group, and by 35% and 40% respectively in Mixtura Dai Huai group as compared with controls. Both drugs appeared to be more effective than colifibrate, in promoting clearance of LDL from plasma suggesting that the role in reducing plasma TC and LDL-C was depended on accelerating LDL degradation through both receptor dependent and independent pathways. It was also suggested that immune stimulus in combination with cholagogus may become a new way for prevention of hypercholesterolemia and atherosclerosis.

The effects of Da Huai Mixture on receptor dependent and independent pathways of LDL catabolism of the experimental hypercholesterolemic rats were observed by isotope trace-labelling, measurement of monocytic macrophage function and dot bloting. The results were as follows: (1) Serum total cholesterol was significantly decrcased(P

Along with the fast development of medical technology,the theory of holistic medical care is infiltrated into every aspect of clinical medical practices.In this paper,though the treatment of surgical hepatobiliary diseases under the direction of holistic medical care ,we emphasize the significance of holistic medical care in diagnosis and treatment ,recommend and canonize the usage of holistic medical care into the clinical practices.

Objective To design a new type of fluorescence detection optical system for dedicated neonatal PKU screening. Methods The system selects super-luminance UVLED for excitation light source, uses different diameter quartz fiber and Y -type optical fiber which consists of glass fiber bundles, and self focusing optical fiber for collimating light and photomultiplier tubes for testing. Results The system has many features, such as simple structure, small size and high sensitivity. Conclusion The clinic screening requirements are met by the system and a new means of rapid detection is provided for PKU screening in large quantities.

Objective To survey the clinical effect of combination therapy of Ninggan-Tiaozhong decoction and psychological intervention for chronic atrophic gastritis (CAG).Methods 80 cases CAG were randomly divided into a treatment group (treated with Ninggan-Tiaozhong decoction and psychological intervention) and a control group (treated with Ninggan-Tiaozhong decoction),with 40 cases in each group and performed a therapy for 12 weeks.The clinical efficacy and the change of Symptom Checklist (SCL-90)and Self-Rating Anxiety Scale(SAS) before and after treatment were evaluated.Results After 12 weeks of treatment,the total effective rate was 90.0％ in the treatment group,and 74.4％ in the control group.The treatment group was better than the control group(x2=4.255,P＜0.05).After psychological intervention,SCL-90 and SAS score (39.5±4.3,1.3± 0.6)was significantly reduced in the treatment group than the control group (51.4±3.2,1.5±0.3),(P＜0.05).Conclusion The combination therapy of Ninggan-Tiaozhong decoction and psychological intervention used in CAG is effective to relieve symptoms and to improve life quality of patients.

Objective A easier and faster rat model of organ injury caused by cardiopulmonary bypass was developed to provide a stable and economical experimental platform for the research of CPB ( cardiopulmonary bypass ) .Methods Twenty male Sprague-Dawley rats weight between (288.8 ±26.1)g were randomly divided into sham group and CPB group .The right external jugular vein and bilateral femoral arteries were punctured in sham group ,while the rats in CPB group underwent 60 minutes cardiopulmoanry bypass with the perfusion flow no less than 120 mL· kg-1· min-1 .And the weight ,blood pressure ,blood gas and electrolytes were monitored and analyzed throughout the experi -ment.The serum,lung,liver and kidney were collected at 72 h after the operation for further detection .Results The rat model of cardiopul-monary bypass was established successfully with 90%survival rate .The changes of blood gas during intraoperative and postoperative were in accordance with clinical reality and the injury of lung ,liver and kidney were confirmed by serum results and pathological changes .Conclu-sion The model has the characteristics of lower weight ,less priming solution ,no need for endotracheal intubation and easier to assemble the bypass line ,furthermore the methods of puncture and CPB were exhibited through a large number of pictures .As a consequence ,the difficul-ties of establishing the rat model of CPB were reduced significantly .The changes of pathophysiology during intraoperative and postoperative were similar to clinical realities ,the survival rate and organ injury were satisfactory .So it can be used as an effective tool for the research of organ injury and the evaluation of therapeutic strategies associated with CPB .

Objective To investigate the effect of warfarin on the formation of renal calcium oxalate stones induced by 1% ethylence glycol (EG) and 3% ammonium chloride (NH 4Cl) in rats. Methods 30 adult male Wistar rats were randomized to three groups,the control group (A) being non-treated,the stone group (B) rats taken EG and NH 4Cl,and the warfarin group (C) taken warfarin in addition to EG and NH 4Cl.All rats were bred 4 weeks.The renal tissue calcium,the renal calcium oxalate crystallization,and the urinary calcium excretion and serum biochemical data were detected. Results No significant difference has been noted in 3 groups,although there is a tend that number of ranal calcium oxalate crystals in group C was more than that in group B. Conclusions The results denoted that warfarin had no significant effect on the calcium oxalate crystallization in rats.

Objective To explore the effects of aerobic exercise training on the circulating miRNAs and their roles in cardio‐vascular adaption induced by aerobic exercise training .Methods Ten freshmen were enrolled .All subjects performed an 8 weeks swimming training .VO2 max and cardiovascular function were measured in acute exhaustive before and after the aerobic endurance training .The key circulating miRNs (miR‐133a ,miR‐21 ,miR‐146a ,miR‐199a ,miR‐15a and miR‐222) were measured at rest and im‐mediately following acute exhaustive exercise in competitive male freshmen before and after the aerobic training .Results Distinct patterns of c‐miRNA response to exercise were observed and adhere to 4 major profiles :after aerobic exercise training ,the levels of miR‐146a ,miR‐222 ,miR‐21 ,miR‐15a and miR‐199a at rest were significant higer than before training (P<0 .05) .The levels of miR‐146a and miR‐222 were up‐regulated by acute exercise before and after aerobic exercise training (P<0 .05) .Before the aerobic train‐ing ,acute exhaustive exercise increased miR‐21 and miR‐15a significantly(P<0 .05) ,however ,there was no difference between the miR‐21 and miR‐15a levels in acute exhaustive exercise and resting state after aerobic exercise training (P>0 .05) .There was no difference in the level of miR‐133a between resting state and acute exhaustive exercise before and after training (P>0 .05) .Further linear correlation analysis showed that the miR‐146a expression in plasma was significantly positively correlated with VO 2 max level (r=0 .842 ,P<0 .01) ,the change of miR‐222 expression in plasma before and after training was significantly positively correlated with EF (r=0 .920 ,P<0 .01) .Conclusion MiRNAs can be used as a marker to reflect the effect of training ,miR‐146a and miR‐222 play a role in physiological regulation of the cardiovascular function of aerobic exercise training in the body .

Objective: In order to reveal the mechanisms of rh-endostatin and its suppression effect on HUVECs at different activating levels. Methods: Three types of HUVEC were prepared. We detected the ex-pression of CD105 and CD62E on the surface of adhering HUVEC, HUVEC of exponential phase of growth, and HUVEC activated with TNF-alpha. The effect of endostatin on these three types of HUVEC was indicated by the variation in the expression of CD105 and CD62E on the cells. The relationship of the changes in cell markers with the time and the concentration of endostatin was analyzed by SPSS 11.5. Results: The expres-sion of CD105 was correlated with time and dose of endostar in all groups. The expression of CD62E was cor-related with the dose of endostar in HUVEC treated with TNF-α for 24h. Conclusion: Endostatin of a certain dose can inhibit the activation of endothelial cells but it cannot induce cell apoptosis.

0.05).Thirty-four cases of newly diagnosed children with CR rate was 88.2% (30 cases).Which the expression of PRAME gene,WT1 gene were both positive and negative groups of children with CR rates were 62.5% and 100.0%,there was no significant difference between the 2 groups(P