1.Cause of death related to medical disputes in Yancheng area: a study of 60 autopsy cases.
Ming-Qi PENG ; Rong-Yu CHEN ; Lan ZHOU ; Kai-Qiao ZHANG ; Jian-Song SHI
Journal of Forensic Medicine 2014;30(2):110-111
OBJECTIVE:
To summarize the pattern and main characteristics of fatal cases related to medical disputes in Yancheng area.
METHODS:
Sixty fatal cases of medical disputes were retrospectively analyzed to elucidate the annual incidence, characters of distribution of hospitals, gender and age of the decedents, types of diseases, and cause of death.
RESULTS:
Among 60 fatal cases, most cases happened in health clinics of county, township and village. There were more males than females. The major medical specialties involved included internal medicine, surgery, gynecology and pediatrics, with the internal medicine specialty having the highest incidence.
CONCLUSION
Police institutions have advantages in investigation of these cases in their jurisdictions, which could enhance the ability of local medicolegal examination.
Autopsy
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Cause of Death
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Dissent and Disputes
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Environment
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Female
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Forensic Medicine
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Humans
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Incidence
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Male
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Medicine
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Police
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Retrospective Studies
2.Influence of high mobility group box 1 on migration of human cord blood CD34(+) cells.
Xin CHEN ; Xing-Bing WANG ; Hui-Lan LIU ; Wen YAO ; Kai-Di SONG ; Zi-Mi SUN
Journal of Experimental Hematology 2009;17(2):422-425
The objective of study was to explore the influence of high mobility group box 1 (HMGB1) on migration of cord blood CD34(+) cells and their mechanism of migration. The expressions of receptor for advanced glycation end products (RAGE), toll-like receptor-2 (TLR2) and TLR4 were detected by flow cytometry. The CD34(+) cells in umbilical cord blood (CB) were enriched by MiniMACS and were exposed to various concentration of HMGB1 (10, 50, 100, 1, 000 ng/ml), then the migration effect of HMGB1 on umbilical cord blood (UCB) CD34(+) cell count was determined by microscopy, the chemotactic index was calculated. The CD34(+) cells untreated with HMGB1 were used as control. The results indicated that the purity of the isolated CD34(+) cells was more than 98%. The HMGB1 could promote the migration of CD34(+) cells, and the migration effect of HMGB1 on CD34(+) cells in certain concentrations gradually increased along with raise of concentration, the strongest effect was observed in concentration of 100 ng/ml, there was significant difference as compared with control (p < 0.01). Anti-RAGE antibody partially inhibited the migration effect of HMGB1 on CD34(+) cells. It is concluded that the HMGB1 in certain concentration can enhance migration of CD34(+) cells, which may be mediated through RAGE.
Antigens, CD34
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Cell Movement
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drug effects
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Cells, Cultured
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Female
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Fetal Blood
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cytology
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drug effects
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HMGB1 Protein
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pharmacology
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Humans
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Receptor for Advanced Glycation End Products
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Receptors, Immunologic
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metabolism
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Signal Transduction
3.Construction of hu-PBL/SCID chimeras and development of EBV-related lymphomas.
Run-liang GAN ; Ke LAN ; Zhi-hua YIN ; Li-jiang WANG ; Ying SONG ; Kai-tai YAO
Chinese Medical Sciences Journal 2005;20(1):16-22
OBJECTIVETo construct hu-PBL/SCID chimeras and to investigate the development of lymphoma and oncogenicity of the Epstein-Barr virus (EBV).
METHODSHuman peripheral blood lymphocytes (PBLs) were isolated from healthy adult donors and transplanted intraperitoneally into severe combined immunodeficient (SCID) mice. Mice with hu-PBL engraftment from healthy EBV seronegative donors were injected intraperitoneally with EBV-containing supernatant from suspension culture of B95-8 cell line (active infection), whereas mice receiving lymphocytes from healthy EBV seropositive donors were not re-infected with B95-8 derived EBV (latent infection). Pathological examination and molecular analysis were performed on experimental animals and induced neoplasms.
RESULTSIn the early stage of this experiment, 12 mice died of acute graft-versus-host disease, mortality was 34.3% (12/35 mice) with an average life span of 17.5 days. In 19 survival hu-PBL/SCID chimeric recipients from 12 healthy donors, tumor incidence was 84.2% (16/19 mice). The average survival time of tumor-bearing mice was 65.5 days. EBV-related neoplasms in SCID mice were nodular tumors with aggressive and fatal features. Histological morphology of tumors exhibited diffuse large cell lymphomas. Immunohistochemistry revealed that LCA (CD45) and L26 (CD20) were positive, but both PS1 (CD3) and UCHL-1 (CD45RO) were negative, and EBV products ZEBRA, LMP1, and EBNA2 were expressed in a small number of tumor cells. EB virus particles were seen in the nuclei of some tumor cells by electron microscopy, and EBV DNA could be amplified in the tumor tissues by PCR. In situ hybridization indicated that the nuclei of tumor cells contained human-specific Alu sequence.
CONCLUSIONSEBV-induced tumors were human B-cell malignant lymphomas. We obtained direct causative evidence dealing with EBV-associated tumor deriving from normal human cells.
Adult ; Animals ; Antigens, CD20 ; metabolism ; Chimera ; Epstein-Barr Virus Infections ; immunology ; virology ; Graft vs Host Disease ; prevention & control ; virology ; Herpesvirus 4, Human ; physiology ; Humans ; Leukocyte Common Antigens ; metabolism ; Leukocyte Transfusion ; methods ; Lymphoma, B-Cell ; immunology ; virology ; Lysosomal-Associated Membrane Protein 1 ; metabolism ; Mice ; Mice, SCID
4.Application and comparison of two shade guide training systems
Feng LIU ; Tong-Kai XU ; Ming-Ming XU ; Hai-Lan FENG ; Guang-Ying SONG
Chinese Journal of Stomatology 2009;44(11):645-648
Objectives To evaluate the effect of shade guide training box and shade guide training software on shade matching ability of observers when used separately.To find out the difference between two training plans when the two training methods were used in combination,and to provide information on shade matching training system. Methods Sixty-two postgraduate dental students who specialized in prosthodontics with 1 to 5 year clinical experience were enrolled in this study.At base Iine.each participant were asked to match 7 standard shade tabs which have been randomly chosen from Vita 3D-Master shade guide and 7 intermediate shade tabs from Vita bleached guide 3D-Master.Then the subjects were allocated to 2 groups[Toothguide Training Box(TTB)group and Toothguide Training(TT)group]according to the baseline data.Participants in group,TTB received training session once a week for 3 weeks.while those in group TT received TT training session once a week for 3 weeks.All participants took a middle term shadematching test. Then the two groups exchange the training methods and repeat the whole process,a final test was given to each participant.The elapsed time and number of accurate shade matching were recorded for each training session.Wilcoxon signed ranks test and ANOVA were used in data analysis.Results There were no significant differences in the number of accurate shade matching(standard shade tab and the sum)between group TTB(4.4±1.3 and 5.3±1.6)and TT(4.0±1.4 and 4.9±1.5)in the middle term test with higher value found in group TTB.In the final test.the number of accurate shade matching(standard shade tab and the sum)in group TT(4.9 ±0.8 and 6.4±0.8)was higher than that in group TTB(4.7±1.1 and 5.8 ±0.9).but significant difference was found only when the sum number of accurate shade matching was compared between the two groups(P<0.05).There was no significant difference between data from middle term test and from final test in group TTB:while in group,TT,the number of accurate shade matching in the final test was,significantly increased compared with that in the middle term test(P<0.05)Conclusions When used in combination,TT training followed by TTB training is recommended.
5.Effective analysis of standard management of intensive treatment by insulin pump to tiabetic patients
Gui-Zhi LI ; Qiu-Ying SUN ; Kai-Lan SONG ; Li-Xiang BIAN
Chinese Journal of Modern Nursing 2008;14(z1):1-4
Objective To analyze standard management study of intensive treatment by insulin pump to diabetes mellitus patients. Methods 60 diabetes mellitus patients of intensive treatment by insulin pump according were divided into experimental group and control group randomly. The patients in experimental were intervented according to "Standard management scheme of intensive treatment by insulin pump to diabetes mellitus patients". Norm:blood glucose controlling, number of days of normal, dose of insulin, hypoglycemic episodes,breakdown, knowledge, technique related insulin pump were evaluated. Results The level of blood glucose in experimental group reached norm much faster (P<0.05). The experimental group' hypoglycemic episodes and dawn phenomenon were lower than the control group (P<0.01). The breakdown related insulin pump in experimental group was lower than the control group sgificantly (P<0.01). The experimental group' theorical and technical knowledge related insulin pump were much better(P<0.05). Conclusions "Standard management scheme of intensive treatment by insulin pump to diabetes mellitus patients" designed reasonably, and accomplished study objective.
6.Long-fragment RNA inhibits hepatitis B virus gene replication and expression in HepG2.2.15 cells.
Lv TIAN ; Song HE ; Xuan LI ; Wen-yan HU ; Pai-lan PENG ; Feng WANG ; Chang-yi GAO ; Hong REN ; Kai-fu TANG
Chinese Journal of Hepatology 2011;19(1):44-47
To evaluate the inhibitory effects of long antisense RNA on HBV replication in HepG2.2.15 cells. The coding region of HBV S gene was cloned into pTARGET vector in sense and antisense orientations and the recombinant plasmids were transfected into HepG2.2.15 cells which were divided into HBS2 (antisense RNA) group, HBS4 (sense RNA) group and control group. HBsAg and HBeAg in the culture supernate were detected by ELISA. The HBV DNA in the supernate was quantified by real-time PCR. After treatment, the levels of HBsAg in HepG2.2.15 cell supernatants of three groups were 0.621+/-0.027, 3.399+/-0.018 and 2.232+/-0.187 respectively; the levels of HBeAg were 0.749+/-0.019, 1.548+/-0.025 and 1.570+/-0.044 respectively and the levels of HBV DNA were 1.597+/-0.082, 3.381+/-0.297 and 3.610+/-0.063 respectively. The expressions of HBsAg and HBeAg and the HBV DNA level in HBS2 group were remarkably reduced as compared to the control (Z = -2.309, P value is less than 0.05); whereas the sense plasmid transfection (HBS4) did not affect HBeAg (Z = -0.866) and HBV DNA (Z = -1.155) levels in the culture supernate but slightly increased the HBsAg level (Z = -2.309). Antisense RNA might be a useful tool to repress HBV replication.
DNA, Viral
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genetics
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Gene Expression Regulation, Viral
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Genetic Vectors
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Hep G2 Cells
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Hepatitis B Surface Antigens
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analysis
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Hepatitis B e Antigens
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analysis
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Hepatitis B virus
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genetics
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physiology
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Humans
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Plasmids
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RNA Interference
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Transfection
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Virus Replication
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genetics
7.Effects of HMGB1 on human cord blood CD34(+) hematopoietic stem cells proliferation and differentiation in vitro..
Xing-Bing WANG ; Xin CHEN ; Kai-di SONG ; Jian WANG ; Wen YAO ; Hui-Lan LIU ; Zi-Min SUN
Chinese Journal of Hematology 2010;31(2):88-91
OBJECTIVETo study both the release of HMGB1 from irradiation-treated mesenchymal stem cells (MSCs) and the effects of HMGB1 on human cord blood CD34(+) hematopoietic progenitor cell proliferation and differentiation.
METHODSMSCs were obtained from human bone marrow. HMGB1 released by the MSCs after treatment with 12 Gy gamma-ray irradiation was determined by enzyme linked immunosorbent assay (ELISA). CD34(+) cells were positively selected with a MACS CD34 isolation kit. The freshly isolated CD34(+) cells were cultured in the presence of HMGB1 for 6 days. Phenotype of cultured cells surface molecules (CD13, CD14, CD11c, CD41 and CD71) were analyzed by flow cytometry. The proliferation and differentiation capacities of cord blood HSCs were assayed by colony forming cell assay. The receptors of HMGB1 (RAGE, TLR2 and TLR4) on cord blood CD34(+) cells were detected by flow cytometry.
RESULTSHMGB1 level in the supernatant \[(4.3 +/- 0.9) ng/ml\] of the irradiated MSC was significantly higher than that in control \[(0.4 +/- 0.2) ng/ml\] (P < 0.01). Human cord blood CD34(+) cells expressed the HMGB1 receptors RAGE, TLR2 and TLR4. The HMGB1-treated CD34(+) cells contained higher proportions of CD13(+) \[(32.6 +/- 5.9)% vs (18.4 +/- 3.8)%\], CD14(+)\[(25.4 +/- 4.4)% vs (12.6 +/- 2.7)%\], CD11c(+) \[(20.3 +/- 3.9)% vs (9.8 +/- 2.1)%\], CD71(+) \[(47.1 +/- 7.4)% vs (26.6 +/- 4.6)%\] cells compared with control group did. But HMGB1 did not induce the generation of CD41(+) cells \[(1.3 +/- 0.5)% vs (1.1 +/- 0.4)%\]. Furthermore, HMGB1 profoundly induced the growth of BFU-E, CFU-GM and total CFU in a dose-dependent manner, and this effect was partially inhibited by TLR2 and TLR4 antibodies.
CONCLUSIONHuman MSC treated with gamma-ray irradiation can release HMGB1, which can induce the proliferation and differentiation of human cord CD34(+) cells.
Antigens, CD34 ; metabolism ; Cell Differentiation ; Cells, Cultured ; Fetal Blood ; cytology ; HMGB1 Protein ; Hematopoietic Stem Cells ; cytology ; Humans
8.The St. George's Respiratory Questionnaire in lymphangioleiomyomatosis.
Kai-Feng XU ; Lan WANG ; Xin-Lun TIAN ; Yao-Song GUI ; Min PENG ; Bai-Qiang CAI ; Yuan-Jue ZHU
Chinese Medical Sciences Journal 2010;25(3):140-145
OBJECTIVETo examine the correlation between the health-related quality of life measured by the St. George's Respiratory Questionnaire (SGRQ) and the commonly used physiological measures in lymphangioleiomyomatosis (LAM).
METHODSThis study retrospectively analyzed the SGRQ scores and other measures (the Borg scale of breathlessness at rest, 6-minute walking distance, blood oxygen levels, and pulmonary function) of patients diagnosed and confirmed with LAM. Altogether 38 patients between June 2007 and November 2009 were included.
RESULTSThe mean values of the SGRQ three components (symptoms, activity, and impacts) and total scores in the LAM patients were 46.95 +/- 28.90, 58.47 +/- 25.41, 47.89 +/- 29.66, and 51.11 +/- 26.35, respectively. The SGRQ total or component scores were correlated well with the Borg scale of breathlessness, 6-minute walking distance, partial pressure of oxygen in arterial blood, spirometry and diffusion capacity of lung. There were poor correlations between SGRQ score and residual volume or total lung capacity. In our preliminary observation, sirolimus improved the SGRQ total and three component scores and the Borg scale of breathlessness significantly after 101-200 days of treatment (n = 6).
CONCLUSIONSThe SGRQ score in LAM is correlated well with physiological measures (Borg scale of breathlessness, 6-minute walking distance, blood oxygen levels, and pulmonary function tests). The SGRQ could therefore be recommended in baseline and follow-up evaluation of patients with LAM. Treatment with sirolimus, an inhibitor of mammalian target of rapamycin, may improve the quality of life and patient's perception of breathlessness in LAM.
Adult ; Forced Expiratory Volume ; Humans ; Lymphangioleiomyomatosis ; physiopathology ; psychology ; Middle Aged ; Quality of Life ; Residual Volume ; Surveys and Questionnaires ; Vital Capacity
9.Local application of bFGF and sucralfate during continuous tissue expansion.
Ya-lan HU ; Shu-zhong GUO ; Kai-hua LU ; Yan HAN ; Yong-hong LEI ; Bao-qiang SONG ; Yong PAN
Chinese Journal of Plastic Surgery 2003;19(1):39-41
OBJECTIVETo investigate the effect of local application of bFGF and sucralfate during continuous tissue expansion (CTE).
METHODSCTE combined with local administration of bFGF and sucralfate was used in twelve patients with scar and nasal tip defects. Twenty three expanders were placed in the subcutaneous pockets through intralesion short incisions. Continuous expansion began at 1-3 days after expander implantation. The histomorphological changes and epidermal cell proliferation were observed. The clinical results were investigated.
RESULTSThe average inflation time was 8.9 days. The average interval of the two operations was 13.5 days. The average hospitalization was 28.4 days. The average immediate stretch-back rate of the expanded skin was 25.7%. The clinical results were satisfactory without any complications. Histological examinations showed that the epidermal, granular and spinous layer became thicker. The basal cells increased significantly. The dermis thinned slightly and the collagen fibers became thicker. The elastic fiber regenerated significantly. Fibroblast and capillary density increased obviously. The immunohistochemistry analysis showed that the proliferation of epidemic basal cells was significant postoperatively.
CONCLUSIONLocal application of exogenous bFGF and sucralfate during CTE was feasible in patients. It could accelerate tissue expansion and improve the quality of expanded skin flap.
Capillaries ; anatomy & histology ; Cell Proliferation ; Collagen ; analysis ; Dermis ; blood supply ; pathology ; Epidermis ; cytology ; Fibroblast Growth Factors ; pharmacology ; Fibroblasts ; cytology ; Humans ; Length of Stay ; Nose Deformities, Acquired ; surgery ; Sucralfate ; pharmacology ; Surgical Flaps ; blood supply ; Time Factors ; Tissue Expansion ; instrumentation ; methods ; Tissue Expansion Devices ; Treatment Outcome
10.Influence of TLR2 and TLR4 agonists on migration of cord blood CD34(+) cells.
Qian-Song CHENG ; Xing-Bing WANG ; Jian WANG ; Hui-Lan LIU ; Liang-Quan GENG ; Kai-Yang DING ; Zi-Min SUN
Journal of Experimental Hematology 2011;19(2):469-472
This study was aimed to investigate the influence of TLR2 and TLR4 agonists on the migration and adhesion activity of umbilical cord blood (UCB) CD34(+) cells and to explore the underlying mechanism. The expression of TLR2 and TLR4 on UCB CD34(+) cells was detected with flow cytometry. The effect of TLR2 agonist (PAM3CSK4) and TLR2 agonist (LPS) on the migration and adhesion ability of UCB CD34(+) cells was evaluated with chemotaxis and adhesion assays. The results indicated that expression levels of TLR2 and TLR4 were (14.2 ± 3.8)%, (19.6 ± 4.1)% respectively. Compared with the control group, the migration activity of UCB CD34(+) cells toward SDF-1 decreased significantly in LPS group (p < 0.01). The adhesion activity was not altered significantly in LPS group. However, both the migration activity towards SDF-1 and the adhesion activity of UCB CD34(+) cells were not changed significantly in PAM3CSK4 group. Further study found that LPS did not affect the expression level of CXCR4 on CD34(+) cells, but could inhibit the spontaneous migration ability of CD34(+) cells. It is concluded that TLR4 activation can decrease the chemotaxis function of CD34(+) cells towards SDF-1, which may associate with the decreased spontaneous migration ability of CD34(+) cells.
Antigens, CD34
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blood
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Cell Movement
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drug effects
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Cells, Cultured
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Chemokine CXCL12
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Fetal Blood
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cytology
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immunology
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Humans
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Lipopeptides
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pharmacology
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Lipopolysaccharides
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pharmacology
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Toll-Like Receptor 2
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agonists
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Toll-Like Receptor 4
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agonists