AIM:To determine whether imperatorin would enhance the effect of doxorubicin therapy on cervi -cal cancer in vitro.METHODS:The viability of HeLa cells treated with imperatorin and doxorubicin was determined by MTT assay in vitro.The expression of Bcl-2 protein family ( Mcl-1, Bcl-2, Bcl-xL, Bad and Bax ) in HeLa cells treated with imperatorin and doxorubicin was evaluated by Western blot analysis .The apoptosis and mitochondrial membrane poten-tial (ΔΨm ) in the HeLa cells treated with imperatorin and doxorubicin were analyzed by flow cytometry .A Mcl-1 expres-sion vector was constructed , and its role in the cytotoxicity of imperatorin plus doxorubicin to HeLa cells was detected by MTT assay.RESULTS: Addition of imperatorin significantly enhanced the cytotoxicity of doxorubicin to HeLa cells in vitro.Mcl-1 expression was down-regulated by imperatorin but was not influenced by doxorubicin in the HeLa cells .A com-bination of imperatorin and doxorubicin induced apoptosis and ΔΨm collapse more significantly compared with the treatment with imperatorin or doxorubicin alone .Furthermore, the imperatorin-induced sensitization for doxorubicin cytotoxicity to HeLa cells was abolished by the transfection with Mcl-1 expression plasmid .CONCLUSION:The combination of doxoru-bicin with imperatorin enhances the antitumor effect of doxorubicin on cervical cancer cells via targeting Mcl -1.

Objective To evaluate the therapeutic effects of pingyangmycin in treatment of body surface hemangioma in children.Methods The clinical data of 1 658 children patients with hemangioma on body surface in which pingyangmycin was injected between January 1997 and January 2008 were analyzed retrospectively.Results All 1 658 patients were observed for 6-12 months,with average of 10.83 months.The total effective rate was 97.09%.Compared among different types of hemangioma,total effective rate had significant difference(?2=203.12,P

Objective To investigate and compare the perception of communication difficulties by mainstreaming students with hearing impairment and that of their peers.Methods The children’s version of the Abbreviated Profile of Hearing Aid Benefit (APHAB) was used to measure perceived communication difficulty in various daily situations.Results There was significant difference between the perception of mainstreaming students and that of their peers for the background noise(BN) subscale questions.Conclusion In order to improve the communication ability of the students with hearing impairment, it is critical to educate one of their important communication partners—their peers, about the hearing loss and its effect on communication, especially under the situation of background noise.

Objective To evaluate the effect of laparoscopy in treatment of renal cysts.Methods Twenty- eight and thirty-five patients with renal cysts were performed with transperituneal and retroperitonel laparoscopy,re- spectively.Results A total of 62 patients were treated successfully,the average operation time was 60 minutes,no complications presented,and there was no recurrence in follow-up.Conclusion Laparoseopy survey had the advan- tages of less trauma and fewer complications and rapid recovery in treatment of renal cysts,which was suggested for clinical applications.

Objective: To investigate the non-alkaloids in Huperzia serrata. Methods: The constituents were mainly isolated and purified by means of silica gel, RP18, and Sephadex LH-20 column chromatographies. The structures were identified based on analyses of the spectral data of NMR and MS. Results: Totally 11 constituents were obtained from dichloromethane fraction of the plant and they were 3β-hydroxyserrat-14-en-21β-yl-p-dihydrocoumarate (1), seratenediol-3,21-diacetate (2), seratenediol-3-acetate (3), 21-epi-serratenediol-3-acetate (4), 3α,21β,24-trihydroxyserrat-14-en (5), 21-epi-serratenediol (6), serratenediol (7), 3α,21β-dihydroxy- serrat-14-en-16-one (8), 3β,21β,24-trihydroxyserrat-14-en (9), 3α,21β,24-trihydroxyserrat-14-en-16-one (10), and 1-dibenzofuranol (11). Conclusion: Compounds 1-10 are serratene-type triterpenoids and compound 11 is a dibenzofuranol. Compounds 1 is a new compound named as serratcoumarate and compounds 2 and 11 are isolated from the plant for the first time.

Cochlear Implantation ; methods ; Fractures, Bone ; surgery ; Humans ; Temporal Bone ; injuries ; surgery ; Temporomandibular Joint ; surgery

BACKGROUND: There is a complicated relation between somatic disease and mental disorder. Somatic disease may induce functional disorder of brain, and generate psychiatric symptoms due to the change of psychological and social environment. The proportion of depression in elder people based on the chronic somatic disease is much higher, and thus the fatality of them increases.OBJECTIVE: To investigate the influence of single antidepressant, and therapeutic alliance of psychological and drug treatment on the rehabilitation of patients with elderly chronic somatic disease complicated with depression. DESIGN: Randomized and controlled grouping experiment.SETTING: Department of Senile Disease of Tongji Hospital Affiliated to Huazhong University of Science and Technology.PARTICIPANTS: Totally 102 patients were hospitalized in the Department of Senile Disease of Tongji Hospital Affiliated to Huazhong University of Science and Technology from March 2000 to January 2003, and all the patients had sleep disorder to a different extent, and suffered from symptoms of heart and alimentary tract which could not be explained as organic diseases, and chronic pain or systemic symptom. Their score of Zung scale was ＞ 40 points with average of (48.83±4.15)points. The patients were randomly divided into the drug group (n=46) and the combined treatment group (n=56). Each patient had more than two somatic symptoms in the two groups and more than 81.0% of them had three somatic symptoms. METHODS: According to the different primary diseases in patients of the two groups, regular treatment was given routinely. Oral thymoleptics fluoxetine of 20 mg/d 5-hydroxyltryptamine reuptake restrainer with 20 mg/d was administered, the dose of which could be added to 40 mg/d according to the pathogenetic conditions. At the beginning of the administration of oral antidepressant drugs, the patients in the combined treatment group were The focal point of depression and fear was identified, and the comfort and edge related to the disease was supplied, and the pathogenetic condition and the prognosis were told to the patients objectively. The family and society were encouraged to learn the rehabilitative skill and improve their possible, and the family and society were encouraged to give the patients adequate psychological support and life support. The patient and the family were assisted to establish favorable communication. The feeling of loneliness and isolation was reduced, and the sense of safety was increased. The depression status and the quality of life of the patients in the two groups were evaluated before the treatment and at 2 and 8 weeks after treatment with the Zung scale and the Spitzer quality of life index score (QLI), including 5 aspects: Activity, daily life, health, support and perspective. The highest score was 10 points. The lowest was 0, and the higher the score, the better the quality of life is.MAIN OUTCOME MEASURES: The results of the Zung scale and the QLI score evaluated before the treatment and at 2 and 8 weeks after the treatment.RESULTS: The treatment of the patients in the two groups was all finZung scale in the drug group and the combined treatment group at 2 weeks after the treatment indicated that there was significant difference in comparison with those before the treatment (41.12±2.60,48.83±4.15,t =10.67,P ＜ 0.05); (40.88±3.43,49.03±4.21 ,t=11.24,P ＜ 0.05). There was not significant difference in group comparison. The total points of Zung scale at 8 weeks were significantly decreased (36.11 ±2.87 ), (30.71±3.62),and there was significant difference in comparison with that before the treatment. The combined treatment group was better than the drug group in difference of QLI score at 2 weeks between the drug group and combined treatment group before and after the treatment (5.47 ± 1.33,5.32 ± 1.04,5.55±1.18,5.27±1.35). The QLI score of drug group and combined treat ment group at 8 weeks after treatment began to rise 7.21±0.13 and 8.83 ±0.32 ,respectively, and there was significant difference in comparison withthose before the treatment, and there was also significant difference in group comparison.CONCLUSION: The positive and effective combined therapy of anti-depression significantly improved the somatic symptoms of patients, no complications appeared, and most patients presented optimistic attitude to the prognosis of the primary disease. To identify the depression of elder people in earlier period is very important to promote rehabilitation of the primary disease.

Interleukin-17A (IL-17A) is one of the proinflammatory cytokines produced by a subset of CD4+T cells named Th17 cells. It plays a crucial role in both host innate and adaptive immune responses against colonization and infection of respiratory tract bacteria. Studies in animal models have demonstrated that IL-17A can induce the expressions of various chemokines, inflammatory factors and adhesion molecules via multiple intracellular signaling pathways and recruit neutrophils to infection sites. Moreover, recent stud-ies have shown that single nucleotide polymorphisms (SNPs) of IL-17A coding gene can regulate the produc-tion of IL-17A in serum, resulting in individual difference in colonization rate by Streptococcus pneumoniae. This review presents recent data related to the structure, functions and signaling pathways of IL-17A, sum-marizes its roles in host defense based on studies in animal models and discusses the effects of genetic varia-tion of IL-17A on colonization, susceptibility, clinical characteristics and disease prognosis of human respira-tory tract bacteria.

ObjectiveTo investigate the correlation of radiographic parameters and health-related quality of life(HRQOL) instruments before and after thoracolumbar kyphosis correction in ankylosing spondylitis (AS) kyphosis.MethodsThirty-eight AS patients with fixed thoracolumbar kyphosis were treated with pedicle subtraction osteotomies(PSO) in our hospital from January 2004 to March 2010.Pre- and post-operative radiological parameters,Cobb T1-S1,pelvic incidence(PI),pelvic tilt (PT),sacral slope (SS),and sagittal vertical axis(SVA) were measured.The Oswestry disability index(ODI) and scoliosis research society-22(SRS-22) questionnaire were used to evaluate the clinical results.Statistical correlation analysis was made to determine correlation between radiographic parameters and overall or partial specific scores of HRQOL instruments.ResultsThe life quality and related radiological spino-pelvic parameters were improved significantly after operation.Preoperatively,Cobb T1-S1,PT,SS,and SVA had partial correlation with ODI walking score and ODI standing score; postoperatively,only PT and SS had partial correlation with ODI.ConclusionPelvic positional parameters are the key parameters for upright activities,and making pelvis in a neutral position is the ultimate goal of surgery.

With the continuous development of imaging techniques,patients with congenital heart dis-ease,especially in complicated congenital cardiac malformations,have more choices for detection methods of ear-ly diagnosis. Nowadays,the widely used diagnostic imaging methods included echocardiography,CT,MRI,cardi-ac catheterization,and prenatal ultrasound examination. Clinicians should choose the appropriate means of ima-ging test depending on the situation. This article will focus on the clinical application of imaging diagnosis in complicateol congenital heart disease.