Objective To compare the expression of myocardial connexin 43(Cx43)mRNA and its protein during early and late cardioprotection stages of exercise preconditioning.Methods Thirty-two Sprague-Dawley rats were randomly divided into a control group,an exhaustive exercise(EE)group,an early exercise preconditioning + exhaustive exercise(EEP+EE)group and a late exercise preconditioning + exhaustive exercise (LEP+EE) group,each of 8.All groups were given intervention as their group name indicated.Then in situ hybridization and real-time fluorescent quantitative PCR methods were used to detect the changes of myocardial Cx43mRNA and immunohistochemical method and Western blotting were used to detect the changes of Cx43 protein.Results Compared with EE group,there was significant increase in Cx43 mRNA and its protein expression in group EEP+EE and LEP+EE.Compared with EEP+EE group,no significant changes was found in situ hybridization and Cx43 Immunoreactivity in LEP+EE group,neither did significant differences in the expression of Cx43 mRNA and its protein.Conclusion EEP and LEP can significantly promote the expression of myocardial Cx43 mRNA and its protein respectively.However there is no significant changes of myocardial Cx43 mRNA and protein expression between the 2 time phases.It demonstrates that the expression of Cx43 in the early and late stage of myocardial protective effect was consistent with the changes of the early and late phase of the protective effect of EP.

Adult ; Facial Nerve Injuries ; etiology ; Foreign Bodies ; complications ; Humans ; Male ; Neck ; Parotid Gland

[Objective]To evaluate the value of clinical examination and magnetic resonance imaging in diagnosing chronic anterior cruciate ligament injury.[Method]Sixty-five patients with the diagnosis of chronic anterior cruciate ligament injury were retrospectively analyzed about the course of diagnosis and treatment.To gain the primary diagnosis through clinical examination,8 patients were performed magnetic resonance imaging.Finally all the patients were carried out arthroscopic surgery to make a final diagnosis.[Result]Arthroscopy found 53 cases with complete anterior cruciate ligament tears,12 cases with partial anterior cruciate ligament tears.In the complete anterior cruciate ligament tears cases,79.2% patients had positive anterior drawer test,96.2% had positive Lachman test and 92.5 % had positive pivot shift test.In the partial anterior cruciate ligament tears cases,16.7% patients had positive anterior drawer test,50.0% had positive Lachman test and 33.3 % had positive pivot shift test.The accuracy of MRI in diagnosing anterior cruciate ligament tears was 100%.[Conclusion]Clinical examination and magnetic resonance imaging can diagnose chronic anterior cruciate ligament effectively.

Objective To analyze risk factors for grenade throwing fractures and put forward corresponding preventive measures for the fractures during the military training in recruits,so as to reduce the happen in the military training.Methods The research is case-control study.The trial group and the control group (39 patients each) were followed up and investigated.The investigation indicators included height,body mass index (BMI),whether drinking carbonated beverage frequently,literacy,osteoporosis,throwing training score,throwing posture,warm-up sufficiently,region,whether attend often physical exercise before recruitment,exercise strength,and weather factor.Results There were significant differences in the warm-up sufficiency,attending physical exercise before recruitment,exercise intensity,throwing posture,weather factor between trial group and the control group in recruits.The logistic regression analysis showed that the lack of physical exercise before recruitment,strong exercise intensity,nonstandard throwing posture were the risk factors in grenade throwing fractures in recruits.Conclusion Sufficient warm-up,avoiding exhausted exercise and assault exercise,strict training in accordance with the standard throwing posture,regular participation in physical exercise before recruitment and training in warm season are effective methods for preventing grenade throwing fractures in recruits.

Objective To compare the two cell adhesion assay techniques based on 51 Cr release and 3H-TdR incorporation.Methods Firstly,the cells to be tested were cultured to confluence in the 96 well plate for 24 hours. After with 51 Cr or 3H-TdR label, the isotope labeled cells were add into plate wells and incubated for another 4 hours. Then the un-adhered cells were removed by gently washing. The cpm of two assay system were counted, the sensitivity and stability of two methods were compared.Results Assay methods based on 51 Cr release and 3H-TdR incorporation could both reflect the cell adhesion level correctly. In assaying sensitivity and stability showed that the 3H-TdR incorporation assay was better than in 51 Cr release assay.Conclusions Adhesion method based on isotope label could provide good sensitivity and stability. The sensitivity and stability of 3H-TdR incorporation is better than that of 51 Cr release assay.

Ebola virus disease (EVD) is a severe infectious disease caused by Ebola virus in humans and primates. The main clinical features are fever and bleeding. The disease was first identified in Zaire and Sudan in Africa in 1976. Since then, it has caused many large-scale epidemics in Africa. One of the largest and most complex Ebola outbreaks in history was the 2014-2016 outbreak in West Africa, which caused more cases and deaths than all previous outbreaks combined. As of 2022, about 35 000 EVD cases and 15 000 deaths have been reported. During the African pandemic, EVD also spread to other regions outside the African continent, such as the Americas and Europe, and became a public health issue of worldwide concern. In Africa, the re-emergence of the disease in Uganda and the Republic of Congo in 2022 has attracted much attention from the world. This article systematically summarizes the history, epidemiological distribution, route of infection, clinical symptoms, diagnosis, treatment, prevention and control of Ebola virus disease, so as to provide reference for relevant workers in China.

AIM:To establish a method for evaluating the principle components of Herba Artemisiae Annuae from different habitats.METHODS:Using Fourier transform infrared spectrometry(FTIR),the characteristic peaks of fingerprints of samples from 12 habitats were recognized and compared.RESULTS:The samples from 12 habitats were obviourly different in number,frequency and intensity of peaks.CONCLUSION:FTIR is applied to analysis of principle components of Herba Artemisiae Annuae from different habitats and an operable method of quality control and discrimination for them is provided.

Objective To investigate the clinical value of combined detection of the serum levels of CA125,CA72-4 and tumor specific growth factor(TSGF)in the diagnosis of ovary cancer and the curative effect.Methods Serum levels of CA125,CA72-4 and TSGF in 68 patients with ovary cancer,53 patients with benign ovary rumor and 50 normal controls were measured by chemiluminescent immunoassay(CLIA)and chemical colorimetry.Results and clinical data were statisticaly analyzed.Results The levels of CA125,CA72-4 and TSGF in ovary cancer group were higher than that in the benign ovarian tumor group and healthy controls(all P ＜0.01).When use three tumor markers united detection,sensitivity,specificity and accuracy were 91.2％,83.0％ and 87.4％ respectively.The sensitivity and accuracy was higher than any single detection.The specificity with single CA125 testing was consistently.The levels of CA125,CA72-4 and TSGF in patients with ovary cancer were significantly different after surgical treatment for five days(all P ＜0.05).Conclusion The combined detection of the three markers may increase the positive rate in the early diagnosis of ovarian cancer,and help to differentiate the benign and malignant ovarian tumor,but also is valuable to observe the curative rate and postoperative monitor of the ovary cancer.

Objective To investigate changes of serum levels of interleukin(IL)-4,-6,-8 and tumor necrosis factor-?(TNF-?),and probe their clinical significance in children with Henoch-Schonlein purpura(HSP)/ HenochSchonlein purpura nephritis(HSPN).Methods Serum levels of IL-4,-6,-8 and TNF-? of 45 children with HSP/HSPN and 43 healthy children were examined by enzyme linked immunosorbent assay(ELISA),changes and correlation between the aforementioned cytokines in children with HSP /HSPN were analyzed.Results 1.Serum levels of IL-4,-6,-8 and TNF-? of children with HSP were higher than those of control group(P0.05);The serum level of TNF-? was positively correlated to the serum levels of IL-6,-8 of patients with HSP(r=0.670 P

40/h) and non-severe group(n=15,AHI 5-40/h).Anthropometric measurements,fasting plasma glucose,insulin,blood fat,and CT quantitative measurement of abdominal adipose tissue were recorded. Results Insulin resistance index(HOMA-IR) in patients with OSAS was related to hypoxia independently of obesity variables.The severe group was characterized by more serious metabolic disorders and higher prevalence of metabolic syndrome than the non-severe group.OSAS was positively associated with an increased metabolic disorders risk for the severe group versus the non-severe group(OR=8.8).Using the receiver operating characteristic(ROC) curve analysis,waist circumference had the greatest areas under the ROC curves compared with body mass index and neck circumference.The results of multiple stepwise regression of lowest pulse oxygen saturation(LSpO2)during overnight sleep indicated that neck circumference followed by epworth sleepiness score(ESS) entered the equation(P