The syndrome of malignant migrating partial seizures in infancy (MMPSI) is one of the most severe epileptic encephalopathies of infancy onset.It has been firstly reported by Coppola in 1995.There have been approximately 80 cases reported worldwide by now,but there is no case reported in our country.The etiology for MMPSI is still unknown,the diagnosis is difficult,and conventional antiepileptic drugs have little efficacy.This paper reviews the possible causes,clinical characteristics,diagnosis and treatment of MMPSI.

AIM: To establish a simple and repeatable orthotopic animal model of the A549 cell line.METHODS: A549 cells in matrigel were inoculated into left lung or right flank of nude mouse. The growth pattern of A549 tumors and the chemotherapeutic efficacy of carboplatinum were studied. RESULTS: Tumors generated and dispersed widespreadly to contralateral lung. Tumors in lung were less susceptible to carboplatinum than those growing in flank. CONCLUSION: A simple and repeatable orthotopic animal model of the A549 cell line was presented, this allows researchers to evaluate novel compounds and new drugs for treatment of human lung cancer.KEY WORDS A549 cell line; orthotopic model; nude mice

Ghrelin is a small active peptide secreted by gastric fundus cell. As an endogenous ligand of growth hormone receptor,it helps to promote growth hormone secretion,increase food intake and participate in energy metabolism. Recently,studies have indicated that Ghrelin,together with other peripheral signals,participate in the regulation of glucose and lipid metabolism. Insulin resistance (IR) is an important pathophysiological basis of the development of type 2 diabetes mellitus (T2DM),while obesity is the common risk factor of both IR and T2DM. It has been confirmed that Ghrelin has a close and complex connection with obesity and T2DM. Further study about Ghrelin may open up a new way to the diagnosis and treatment of obesity and T2DM.

Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g～325?g for imperatorin and in the range of 50?g～250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.

Forensic Medicine ; Homicide ; Technology

OBJECTIVETo explore the distribution features of Chinese medical syndromes of rheumatoid arthritis (RA) by literature retrieval.

METHODSPertinent articles on treating RA by syndrome differentiation were retrieved from China National Knowledge Infrastructure Databases (CNKI), VIP Chinese Biomedical Journal Database, Guizhou Digital Library, and Duxiu Chinese Academic Periodicals full papers database from January 2000 to December 2011.

RESULTSA total of 33 documents were recruited covering 4 233 cases. Damp-heat blocking collaterals syndrome occupied the top in the occurrence frequency (20 times, 60.61%), followed by deficiency of Gan and Shen syndrome (18 times, 54.55%), intermingled phlegm and blood-stasis syndrome (17 times, 51.52%), wind-cold-damp impediment syndrome (15 times, 45.45%), cold-damp blocking collaterals syndrome (14 times, 42.42%), wind-damp-heat impediment and deficiency of qi and blood syndrome (10 times, 30.30%), and intermingled cold and heat syndrome (9 times, 27.27%). According to the case number of patients, it was sequenced as damp-heat blocking collaterals syndrome syndrome (768 cases, 18.14%), wind-damp-heat impediment syndrome(666 cases, 15.73%), wind-cold-damp impediment syndrome(584 cases, 13.80%), cold-damp blocking collaterals syndrome syndrome (517 cases, 12.21%), intermingled cold and heat syndrome (415 cases, 9.80%), intermingled phlegm and blood-stasis syndrome (364 cases, 8.60%), deficiency of Gan and Shen syndrome (235 cases, 5.55%),asthenia of healthy energy due to lingering arthralgia syndrome (223 cases, 5.27%). The case numbers of remaining syndromes did not exceed 5%.

CONCLUSIONDamp-heat blocking collaterals syndrome was the main syndrome in RA patients, followed by wind-cold-damp impediment syndrome,wind-damp-heat impediment syndrome,cold-damp blocking collaterals syndrome,intermingled phlegm and blood-stasis syndrome, and deficiency of Gan and Shen syndrome.

Arthritis, Rheumatoid ; classification ; diagnosis ; Humans ; Medicine, Chinese Traditional ; Yang Deficiency ; classification ; diagnosis ; Yin Deficiency ; classification ; diagnosis

A case of the H syndrome was reported.An 18-year-old man presented with an 8-year history of progressive cutaneous sclerosis,skin hyperpigmentation,and hypertrichosis on the trunk and extremities.In addition,he suffered from short stature,hearing loss,cardiac anomaly,hepatosplenomegaly,and scrotal masses.Laboratory examination revealed hypogonadism.Cutaneous histopathologic examination showed hyperpigmentation of the basal layer with acanthosis,widespread fibrosis (moderate in dermis and severe in subcutis) and an infiltrate composed of histiocytes and plasma cells.A perivascular lymphocyte infiltrate was also observed throughout the dermis and subcutaneous fat tissue.These findings were consistent with the H syndrome.Differential diagnosis must be made with Winchester syndrome and polyneuropathy, organomegaly,endocrinopathy,M-protein and skin change (POEMS) syndrome.

Radiotherapy is one of the most important treatment method for esophageal cancer. Cene expression profile of cancer cells may be one of key factors in determining the effectiveness of radiation therapy To provide guidance for individual therapy, research advances in gene expression involved in radiosensitivity of esophageal cancer are discussed from three aspects: radiation damage repair, cell cycle distribution and signal traneduetion.

Objective To investigate the effect of dexmedetomidine combined with sufentanil on postoperative analgesia and the vascular crisis in patients undergoing free flap transplantation. Methods Forty patients with ASA physical statusⅠ-Ⅱhaving underwent free flap transplantation were divided into groupⅠand groupⅡaccording to the random digits table method with 20 cases each. The drugs of patient controlled intravenous analgesia (PCIA) in groupⅠwere sufentanil 1.0μg/(kg·d)+tropisetron 5 mg/100 ml, and the drugs of PCIA in group Ⅱ were sufentanil 1.0 μg/(kg · d) +dexmedetomidine 1.0 μg/(kg · d) + tropisetron 5 mg/100 ml. The PCIA pump was set in 2 groups as follows: continuous perfusion rate 2 ml/h, single bolus 2 ml, lockout time 15 min. The visual analogue score (VAS), Ramsay sedation score, press number of PCIA pump within 48 h after operation, satisfaction score after operation, untoward reaction and vascular crisis were recorded. Results The VAS at 6, 12, 24 and 48 h after operation in group Ⅱ were significantly lower than those in group Ⅰ: (2.18 ± 0.41) scores vs. (3.00 ± 0.63) scores, (2.64 ± 0.51) scores vs. (3.82 ± 0.60) scores, (2.55 ± 0.52) scores vs. (3.36 ± 0.51) scores and (2.27 ± 0.47) scores vs. (3.09 ± 0.70) scores, the Ramsay sedation scores were significantly higher than those in group Ⅰ: (2.42 ± 0.51) scores vs. (2.00 ± 0.58) scores, (2.74 ± 0.45) scores vs. (2.11 ± 0.57) scores, (2.58 ± 0.51) scores vs. (2.05 ± 0.52) scores and (2.53 ± 0.51) scores vs. (2.00 ± 0.47) scores, and there were statistical differences (P<0.05). The press number of PCIA pump within 48 h after operation in groupⅡwas significantly lower than that in groupⅠ:(3.75 ± 1.45) times vs. (8.25 ± 2.61) times, the satisfaction score after operation was significantly higher than that in groupⅠ:(2.47 ± 0.51) scores vs. (1.60 ± 0.50) scores, and there were statistical differences (P<0.05). There were no statistical differences in the incidences of untoward reaction and vascular crisis (P>0.05). Conclusions Dexmedetomidine can relieve anxiety and nervousness, reduce postoperative consumption of sufentanil, and decrease the incidence of nausea and vomiting. In addition, dexmedetomidine does not affect the incidence of vascular crisis.

Objective To verifying the characteristics of optical coherence tomography(OCT) in exudative age related macular degeneration (AMD). Methods The patients being investigated in this series included 16 cases (19 eyes) of exudative age related macular degeneration diagnosed by FFA and OCT examinations, among them 4 cases (6 eyes) were examined with ICGA. The color photographs of ocular fundi, FFA, ICGA and OCT were investigated by contrasting each other. Results As compared with the FFA and ICGA examinations, the characteristic findings found in OCT in patients with exudative AMD in this series were as the following:①serous detachment of neurosensory epithelium in 11 eyes,②retinal hemorrhage in 2 eyes,③serous detachment of retinal pigment epithelium in 5 eyes,④hemorrhagic detachment of retinal pigment epithelium in 10 eyes,⑤disciform scar in 4 eyes,⑥fibrovascular pigment epithelial detachment and occult CNV in 12 eyes. Conclusion OCT can supply a comprehensive survey of exudative AMD, in making the diagnosis as an important complementary examination of FFA and ICGA.