No abstract available.
Agglutination* ; Alisma* ; Animals ; Erythrocytes* ; Mice* ; Plantago*

BACKGROUND:In China there is no norm to assess anxiety disorders in children.The Chinese urban norm for screening child anxiety related emotional disorders is applicable to assessing anxiety problem in Chinese children and assisting clinical diagnosis,research and epidemiological investigation. OBJECTIVE:To develop the norm for screening child anxiety related emotional disorders(SCARED) in Chinese cities,and test the reliability and validity of this scale. DESIGN:A sampling survey taking the students of Grade 2 and up in elementary schools and junior middle schools in 14 big and medium sized cities in China and children with anxiety disorders who visited children's mental clinics in Changsha,Jinan and Shenzhen cities from October 2001 to April 2002,as the subjects. SETTING:The psychiatric department of a provincial specialized mental hospital. PARTICIPANTS:A total of 2 019 subjects(1 012 males and 1 007 females) with the mean age of(11± 2) years old were selected from 14 cities in China(Beijing,Tianjin,Taiyuan,Siping,Wuhan, Changsha,Xi'an,Shenzhen,Mianyang,Jinan,Fuzhou and Yichun) from October 2001 to April 2002.The students and their parents(norm group) filled in SCARED,and meanwhile,90 children with anxiety disorders who visited children's mental clinics in Changsha, Jinan and Shenzhen were taken as anxiety group. INTERVENTIONS:The children completed one copy of SCARED in the classroom during regular classes after informed consent was obtained, and then the questionnaires were collected on the spot. Another copy of SCARED was taken home by the children to be completed by the parents.Meanwhile,in Changsha,the parents filled in the child behavior checklist(CBCL) while their children completed the Piers Harris children's self concept scale(PHCSS).The reliability and validity of SCARED were determined. MAIN OUTCOME MEASURES:Reliability and validity of SCARED. RESULTS:The test retest coefficients of SCARED ranged from 0.567 to 0.608,split reliability of SCARED was 0.88,and Cronbach alpha(α ) coefficients were 0.43 to 0.89,with good reliability. Significant correlations were found between the total scores and anxiety indexes of SCARED and Achenbach CBCL(r=0.564,0.603,P< 0.05).The children in anxiety group scored higher in SCARED than those in norm group(t=8.06 to 12.89,P=0.000). Sensitivity and specificity of diagnosis of anxiety disorder were 0.74 and 0.79,respectively. CONCLUSION:The reliability and validity of SCARED are good, suggesting that the norm is applicable to assessing anxiety disorders in Chinese children.

The syndrome of malignant migrating partial seizures in infancy (MMPSI) is one of the most severe epileptic encephalopathies of infancy onset.It has been firstly reported by Coppola in 1995.There have been approximately 80 cases reported worldwide by now,but there is no case reported in our country.The etiology for MMPSI is still unknown,the diagnosis is difficult,and conventional antiepileptic drugs have little efficacy.This paper reviews the possible causes,clinical characteristics,diagnosis and treatment of MMPSI.

Recently, we are confronted with prblems of dcelince in emplpyment rate,increase in contract breach and empleyement imbalance among different majors, ect. In this paper, we improved em-ployment work from the apects of implementation concept and methods of occupation career planning ed-ucation, entrepreneurship education, employment information system construction and quality feedback mechanism construction.

BACKGROUND:At present, the placement of tibial anatomic landmarks has no gold standard during total knee arthroplasty. In order to achieve the most ideal rotation function of the tibial prosthesis, we should do the preparation before surgery, understand tibial rotational alignment to rationaly select and apply the prosthesis.
OBJECTIVE:To investigate the effect of tibial rotational alignment technique on patelofemoral joint biomechanics and related mechanisms.
METHODS:Ten pairs of human knee joint specimens were colected to prepare experimental platform. Specimens were fixed on the experiment frame, which was fixed on the material testing instrument for mechanics experiment. Weset different knee flexion angles, including 30°, 60°, 90° and 120°. Joint replacement was performed with the knee prosthesis. Samples were randomly assigned to two groups (n=5). Tibial nodule technology and ROM technology were used to identify rotationalalignment of the tibial prosthesis. The medial and lateral patelofemoral joint contact pressure peak and patelofemoral contact area at different knee angles, and the medial and lateral parts of patelofemoral contact area at deep knee angles were observed in both groups.
RESULTS AND CONCLUSION:(1) During flexion angle from 30° to 60°, the peak contact pressure of medial patelofemoral joint increased, and decreased gradualy in both groups. At flexion angle of 90°, contact pressure reached the minimum value, then increased gradualy, and reached the maximum value at 120°. No significant difference in peak contact pressure of medial patelofemoral joint at different knee angles was detected between the two groups (alP> 0.05). (2) During flexion angle from 30° to 60°, peak contact pressure of lateral patelofemoral joint decreased constantly, and then gradualy increased. No significant difference in peak contact pressure of lateral patelofemoral joint at different knee angles was detectable between thetwo groups (alP> 0.05). (3) During flexion angle from 30° to 60°, patelofemoral contact area decreased, and then increased gradualy in both groups. No significant difference in patelofemoral contact area at different knee angles was identified between the two groups (alP> 0.05). (4) At 90° and 120°, no significant difference in the area of medial and lateral contact parts was determined between the two groups (alP> 0.05). (5) Experimental results showed that tibial nodule technology and ROM technology for identifying tibial rotational alignment during total knee arthroplasty can obtain considerable biomechanical effects of patelofemoral joint.

Objective] Summarize clinical experience about treatment of scapulohumeral periarthritis with neck rotation and shoulder movement proposed by the well-known doctor Wang Youren from Beijing Massage Hospital. [Method] Observe the clinical treatment of scapulohumeral periarthrit, organize the related medical records and draw on the treatment thoughts and operational method.[Result] Mr. Wang thinks that there is close connection between the scapulohumeral periarthriti and neck in the perspective of pathogenesis and anatomy and physiology. It will have better treatment effect if giving the fine-tuning to the neck while treating the scapulohumeral periarthritis. [Conclusion] Mr. Wang Youren has unique clinical treatment experience in the area of scapulohumeral periarthritis, and this treatment has significant effect. Thus it is worth of spreading.

Objective To study plasma levels of tumor necrosis factor-α(TNF-α) ,IL-6 ,IL-10 ,serum phospholipase A2(PLA2) and organ lesion of sever burn injury patients .Methods 57 sever burn injury patients got treated in our department from March 2010 to March 2011 ,in which 33 of them had been diagnosed with sepsis and 19 of which developed into multiple organ dysfunction syndrome(MODS) ,the levels of IL-6 ,IL-10 ,TNF-α,PLA2 were compared among sepsis group(group N) ,MODS group(group M) and negative group(group N) .The organ disfunction and fatality rate were also analyzed .Results The PLA2 ,TNF-α,IL-6 levels of patients in group S and M were significantly higher than group N ,and showed no apparent decline during the first 14 day ;the change trend of IL-10 had no statistically significant difference among the 3 groups(P<0 .05) .The group S and M showed larger burned area and deeper burned depth ,the organ failure and mortality rate were significantly higher than group N ,And with the most common respiratory function failure .Conclusion The PLA2 ,TNF-α,IL-6 levels in sever burn injury patients might be closely relat-ed to the degree of burn .High plasma level of PLA2 ,TNF-α and IL-6 might be closely associated with occurrence of sepsis and MODS and their outcome .

Objective:To investigate the effect of PEG-PEI/Fe3O4 Nano-magnetic fluid-TK on Apoptosis of liver cancer cells(HepG2)in vitro.Methods:Construcr ecombinant plasmid PEGFP-AFP-TK was constructed,which was delivered by nano-magnetic fuilds into AFP positive HepG2 cells and AFP negative SMMC7721 cells.The fluorescence was detected in order to evaluate the transfection rate 12h after transfection RT-PCR and Western blot were used to detect expression of HSV-TKgene at 48 h after transfection.MTT assay was used to evaluate the effect of HSV-TK on the proliferation of HepG2 cells,Flow cytometry was used to analyze the apoptosis of HepG2 cells.Results:Nano-Magnetic fluids delivered plasmid PEGFP-AFP-TK into HepG2 cells and HSV-TK gene was successfully expressed.The transfection eficacy of nano-magnetic fluids was superior than lipofectamine in HepG2 cells.RT-PCR and Western blot demonstrated that TK gene was expressed in HepG2 cells after being transfected with nano-magnetic fluids/PEGFP-AFP-TK.MTT and flow cytometry showed HSV-TK gene exerts a cell-killing effect.Conclusions:Nano-Magnetic fluids could successfully deliver PEGFP-AFP-TKinto HepG2 cells and induce expression of HSV-TK,it may become a promising gene vector for liver cancer gene therapy.AFP enhancer can specifically enhance the expression of target gene within the cell with positive AFP.

Objective:To prepare semiconductor quantum dots (QDs)-Smad4 monoclonal antibody Fluorescent probes ,and to detect nucleation shifting process of Smad4 signal protein in Rat dental papillae cells(RDPCs) stimulated by TGF-?1 with the Fluorescent probes. Methods:①QDs were chemically modified with Smad4 proteins to prepare water soluble QDs-Smad4 monoclonal antibody Fluorescent probes which were purified after the preparation. ②The properties of these probes were studied through absorption band analyses,fluorescence spectra,fluorescence spectrum analyses and confocal laser scanning fluorescence microscopy. ③The location of Smad4 proteins in RDPCs was studied with SP anti-Smad4 immunocytochemical method,QDs direct mark method,Smad4 monoclonal antibody direct mark method,and direct immunofluorescence imaging.And the properties of Quantum Dots-Smad4 monoclonal antibody Fluorescent probes in RDPCs were detected. ④After RDPCs ware cultivated by TGF-?1 for 0 hours,12 hours,and 14 hours,the dynamic nucleation shifting process of Smad4 signal protein in RDPCs was separately detected with SP anti-Smad4 immunocytochemical method and direct immunofluorescence imaging.Results:QDs and monoclonal antibody ware covalently bonded to form the fluorescent probes which could specifically and effectively recognize Smad4 proteins in RDPCs .It is to demonstrate the dynamic nucleation shifting process of Smad4 signal protein in RDPCs. These Fluorescent probes still had good properties,including broad excite spectra,narrow emission spectra,high fluorescence intensity,and photostability. Conclusion:QDs and monoclonal antibody could be covalently bonded to form the fluorescent probes with distinct optics character,special immunity recognition capability and the ability of imaging mark the protein in cells for long time,which provides the scientific evidence that QDs can visually research the movement and interaction of protein in living cell .

Objective:To reconstruct pcDNA3.1/VEGF165 and combine it with deproteinized bone(DPB) to repair avascular necrosis of the femoral head (AVNFH).Methods:Clone plasmid pUC18/VEGF165 containing the whole cDNA sequence of VEGF165 was combined with pcDNA3.1(+) to reconstruct pcDNA3.1/VEGF165.Sixty-nine AVNFH model New Zealand adult rabbits with a mean weight of 2.8 kg were randomly divided into three groups.In group 1,DPB combined with the reconstructed plasmid pcDNA3.1/VEGF165 was implanted in the drilled channel of the necrotic femoral head.In group 2,only DPB was implanted.In group 3,only channel was drilled without DPB or plasmid implanted.Femoral head specimens were obtained 3 days,1,2,4,and 8 weeks after operation.The expression of vascular endothelial growth factor (VEGF) was examined by RT-PCR,Western blotting techniques.Angiogenesis and repair of the femoral head were observed by histological and histomorphometric analysis.Results:pcDNA3.1/VEGF165 was reconstructed successfully.In group 1,the expression of VEGF was detected in the femoral head implanted by DPB-VEGF compound.VEGF165 mRNA and protein detected went up to apex 1 week postoperatively and lasted more than 3 weeks.The femoral head implanted by DPB-VEGF compound showed a significant difference in vascularization 2 and 4 weeks after operation and a significant difference in bone formation 2,4 and 8 weeks after operation from those in other groups on histomorphometric analysis (P