1.Detection of RNA of SARS coronavirus in hospital sewage.
Xin-Wei WANG ; Jin-Song LI ; Ting-Kai GUO ; Bei ZHEN ; Qing-Xin KONG ; Bang YI ; Zhong LI ; Nong SONG ; Min JIN ; Wen-Jun XIAO ; Xiu-Mei ZHU ; Chang-Qing GU ; Jing YIN ; Wei WEI ; Wei YAO ; Chao LIU ; Jian-Feng LI ; Guo-Rong OU ; Min-Nian WANG ; Tong-Yu FANG ; Gui-Jie WANG ; Yao-Hui QIU ; Huai-Huan WU ; Fu-Huan CHAO ; Jun-Wen LI
Chinese Journal of Preventive Medicine 2004;38(4):257-260
OBJECTIVEIn order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.
METHODSA novel electropositive filter was used to concentrate the SARS-CoV from the sewage of two hospitals administered SARS patients in Beijing, including twelve 2,500 ml sewage samples from the hospitals before disinfection, and ten 25,000 ml samples after disinfection; as well as cell culture, RT-PCR and sequencing of gene to detect and identify the viruses from sewage.
RESULTSThere was no live SARS-CoV detected in the sewage in this study. The nucleic acid of SARS-CoV had been found in the 12 sewage samples before disinfection from both hospitals by semi-nested PCR. After disinfection, SARS-CoV RNA could only be detected from the samples from the 309th Hospital, and the others were negative.
CONCLUSIONIt provides evidence that there is no live SARS-Cov in the sewage from hospitals with SARS patients though SARS-CoV RNA can be detected.
Hospitals ; Humans ; Nucleocapsid ; analysis ; RNA, Viral ; analysis ; Reverse Transcriptase Polymerase Chain Reaction ; SARS Virus ; genetics ; isolation & purification ; Severe Acute Respiratory Syndrome ; virology ; Sewage ; virology
2.Assessment of the horizontal semicircular canal function after cochlear implantation by video head impulse test and caloric test.
Jie ZENG ; Hong Ming HUANG ; Xiao Qian WANG ; Kai Bang ZHONG ; Pei Na WU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2018;32(2):86-90
To analyze the functional change of horizontal semicircular canals after cochlear implantation.Eighteen patients were enrolled in this study.Their vestibular function was evaluated by using the caloric test and video head impulse test before and one week,one month after CI surgery,respectively.The unilateral weakness(UW),slow phase velocity(SPV)in caloric test and gain in video head impulse test(vHIT-G)were observed.Caloric test was abnormal when UW>25% or SPV mean<6°/s,while vHIT was abnormal when vHIT-G<0.8.The SPV of the implanted ear were[(10.36±8.01)°/s;(14.77±14.24)°/s]pre-operatively,[(6.45±7.52)°/s;(5.14±4.67)°/s]1 week post-operatively and[(6.05±3.86)°/s;(6.27±4.17)°/s]1 month post-operatively.Statistically significant difference(<0.05)was found between pre-and post-operative period.The vHIT-G of the implanted ear were(0.73±0.33)pre-operatively,(0.65±0.32)1 week post-operatively and(0.71±0.36)1 month post-operatively.There was no statistically significant difference of vHIT-G between preand post-operative period((pre-operative/1 week post-operative)=0.084,(pre-operative/1 month post-operative)=0.679).Four patients presented with vertigo and one of them manifested slight unsteadiness post-operatively.All symptoms resolved within 7 days.These symptoms had no correlate with age,gender,implantedear and results of vestibular test.Cochlear implantation can affect the horizontal semicircular canal function,and the video head impulse test and caloric test should be used in a complementary fashion.
Caloric Tests
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Cochlear Implantation
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adverse effects
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methods
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Head Impulse Test
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Humans
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Semicircular Canals
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physiopathology
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Vertigo