Objective This study aims to compare the sedative effects of remimazolam and midazolam during im-pacted tooth extraction to provide a comfortable sedation treatment for patients with dental anxiety.Methods A pro-spective randomized controlled trial was conducted,in which 60 patients undergoing intravenous sedation for mandibular impacted third molar extraction were evenly divided into either the remimazolam or midazolam group.Prior to receiving a nerve blocker,the patients were sedated with remimazolam or midazolam.Various parameters were recorded and ana-lyzed,including onset time,awakening time,recovery time,modified dental anxiety scale(MDAS)scores before and af-ter surgery,patient-doctor satisfaction levels,postoperative side effects within 24 hours,heart rate(HR),and mean arteri-al pressure(MAP)at different time points.Results Compared with the midazolam group,patients in the remimazolam group demonstrated significantly shorter onset,awakening,and recovery times as well as lower postoperative MDAS scores and higher levels of patient-doctor satisfaction.Fewer postoperative side effects were reported in the remimazol-am group,although the differences were not statistically significant.Conclusion The use of remimazolam demon-strates faster onset and recovery,superior efficacy in reducing dental anxiety,and enhanced satisfaction among patients and doctors,thereby presenting distinct advantages for sedation treatment for patients with dental anxiety.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.

The scientific basis of acupuncture on mesenchymal stem cells (MSCs) for treating ischemic stroke (IS) is discussed. MSCs transplantation has great potential for the treatment of tissue damage caused by early stage inflammatory cascade reactions of IS, but its actual transformation is limited by various factors. How to improve the homing efficiency of MSCs is the primary issue to enhance its efficacy. As such, the possible mechanisms of acupuncture and MSCs transplantation in inhibiting inflammatory cascade reactions induced by IS are explored by reviewing literature, and a hypothesis that acupuncture could promote the secretion of stromal cell-derived factor-1α (SDF-1α) from ischemic foci to regulate SDF-1α/CXC chemokine receptor 4 (CXCR4) axis, thereby improving the homing efficiency of MSCs transplantation, exerting its neuroprotective function, and improving the bed transformation ability, is proposed.
Humans ; Ischemic Stroke ; Chemokine CXCL12 ; Acupuncture Therapy ; Mesenchymal Stem Cells ; Inflammation

In this paper, the domestic and international demand and development trend of clinical diagnostic radionuclides are analyzed, and the medium and high-energy cyclotrons, adequate and systematic facilities, and preparation techniques required for the production of medical radionuclides based on solid targets are introduced. This paper focuses on the research and development carried out by some important medical institutions and scientific research institutes in China over the years in the aspects of medium and high-energy cyclotrons, beam transmission lines, high-power irradiation target stations and new medical isotope production processes etc. It also looks forward to some new directions for the development of medical radionuclides in China during the 14th Five-Year Plan period.

OBJECTIVE: This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds. METHODS: The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the cocul-ture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks. RESULTS: The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes. CONCLUSIONS The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.
Adipose Tissue ; Animals ; Cartilage ; Chondrocytes ; Coculture Techniques ; Rabbits ; Regeneration

Background: Cerebral palsy (CP) has no cure yet. This study was aimed to evaluate the efficacy and safety of stem cell therapy (SCT) for improving the gross motor function (GMF) of patients with CP. Methods: A systematic literature search was performed in CENTRAL, PubMed, Embase, and Google Scholar to identify relevant randomized controlled trials from the year 2010 to 2020. The outcome measures were GMF and adverse events. For the meta-analysis, treatment effects on GMF improvement were expressed as standardised mean differences (SMD) with 95% confidence intervals (CI), using a random-effects model. Results: There were seven trials that either used autologous or allogenic stem cells, with 411 participants, and were met with inclusion and exclusion criteria. The age, severity, and type of CP in participants varied. Follow up duration ranged from 6 to 24 months. Four studies had single transplantation while the other three had two to four sessions. Overall, a significant positive effect on GMF was seen in SCT than control group, SMD = 2.22 [95% CI 1.15 - 3.29] with a high heterogeneity (I² = 95%). In a separate analysis, umbilical cord blood (UCB) was the most effective cell type, SMD = 3.24 [1.38,5.10]. Serious adverse events were rare, with similar effects in treatment and control groups. Conclusion: A positive and safe effect of SCT, specially UCB on GMF, was observed. However, the standardizations of treatment regimes, therapeutic-cell dose, and SCT optimal timing are needed to maximize the efficacy of treatment.

Alkaloids from Ba lotus seeds (ABLS) are a kind of important functional compounds in lotus seeds. The present study was designed to determine its hypertension prophylactic effects in the L-NNA-induced mouse hypertension model. The mice were treated with ABLS, the serum and tissues levels of NO, MDA, ET-1, VEGF, and CGRP were determined using the experimental kits, the mRNA levels of various genes in the heart muscle and blood vessel tissues were further determined by RT-PCR assay. ABLS could reduce the systolic blood pressure (SBP), mean blood pressure (MBP), and diastolic blood pressure (DBP), compared to that of the model control group. After ABLS treatment, the NO (nitric oxide) contents in serum, heart, liver, kidney and stomach of the mice were higher than that of the control mice, but the MDA (malonaldehyde) contents were lower than that of the control mice. The serum levels of ET-1 (endothelin-1), VEGF (vascular endothelial growth factor) were decreased after ABLS treatment, but CGRP (calcium gene related peptide) level was increased. The ABLS treated mice had higher mRNA expressions of HO-1, nNOS, and eNOS and lower expressions of ADM, RAMP2, IL-1β, TNF-α, and iNOS than the control mice. Higher concentration of ABLS had greater prophylactic effects, which were close to that of the hypertension drug captopril. These results indicated the hypertension prophylactic effects of ABLS could be further explored as novel medicine or functional food in the future.
Alkaloids ; administration & dosage ; Animals ; Blood Pressure ; drug effects ; Disease Models, Animal ; Humans ; Hypertension ; chemically induced ; drug therapy ; metabolism ; physiopathology ; Interleukin-1beta ; genetics ; metabolism ; Male ; Mice ; Mice, Inbred ICR ; Nitroarginine ; Nymphaeaceae ; chemistry ; Seeds ; chemistry ; Tumor Necrosis Factor-alpha ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; genetics ; metabolism

Objective The purpose of this study is to explore the efficacy and predictors of second?line chemotherpy in advanced non?small cell lung cancer patients and suggest optimal protocols suitable for differently characterized patients. Methods The clinical data of 178 advanced NSCLC patients second?line?treated in Tianjin Cancer Hospital from 2009.1.1 to 2013.12.31 were retrospectively analyzed. According to the different second?line treatments, the patients were divided into standard mono?drug therapy group ( 46 cases), endostar combined with standard mono?drug therapy group (42 cases), and platinum?based doublet chemotherapy group ( 90 cases) . Kaplan?Meier and Log?rank analyses were used to estimate and compare the survival rates in the groups, and Cox′s hazard regression model was used to determine the prognostic factors. Chi?square test was used to analyze the differences among different groups. Results The median progression?free survivals ( mPFS) were 50 days, 54 days, and 79 days ( P=0.042) for the standard mono?drug therapy group, endostar combined with standard mono?drug therapy group, and platinum?based doublet chemotherapy group, respectively. The differences between the mono?drug therapy group and doublet chemotherapy group were statistically significant ( P=0. 011 ) . The disease control rate ( DCR ) for each group was 26.1%, 47.6% and 46.7% (P=0.041), and the DCR were statistically significantly different between the mono?drug therapy group and doublet chemotherapy group ( P=0.016) , and between the mono?drug therapy group and endostar combined with standard mono?drug therapy group (P=0.041). The overall response rate (ORR) for each group was 2.2%, 0, and 4.4% (P>0.05 for all). Multivariate analysis showed that the period from the begining of first?line to second?line chemotherapy ( progression?free time) , base?line clinical stage, neuron specific enolase ( NSE) before second?line therapy, the cycles of second?line chemotherapy and the response to second?line therapy were independent predictors for PFS ( P<0. 005 for all) . Subgroup analysis indicated that the patients obtained more clinical benefit from doublet chemotherapy rather than mono?drug therapy, with following factors: age<60 years, paclitaxel plus cisplatin for first?line treatment, chemotherapy cycles≤4, CR, PR and SD for response, progression time within 3?6 months from the begining of first?line to second?line chemotherapy, performance status score≤1 at the begining of second?line therapy,Ⅳ stage, and mild leukopenia ( P<0.05 for all) . The patients whose progression?free survival time within 3?6 months from the begining of the first?line to second?line chemotherapy got more clinical benefit from endostar combined with standard mono?drug chemotherapy than mono?drug therapy ( P=0.006) . Conclusions The period from the begining of first?line to second?line chemotherapy, base?line TNM stage, NSE before second?line chemotherapy, the cycles of second?line chemotherapy and the response to second?line therapy were independent predictors for PFS. Platinum?based doublet chemotherapy and endostar plus standard second?line regimen can improve the efficacy in some characterized advanced NSCLC as compared with the patients by standard mono?drug therapy, wherein the platinum?based chemotherapy revealed the best efficacy.

Objective The purpose of this study is to explore the efficacy and predictors of second?line chemotherpy in advanced non?small cell lung cancer patients and suggest optimal protocols suitable for differently characterized patients. Methods The clinical data of 178 advanced NSCLC patients second?line?treated in Tianjin Cancer Hospital from 2009.1.1 to 2013.12.31 were retrospectively analyzed. According to the different second?line treatments, the patients were divided into standard mono?drug therapy group ( 46 cases), endostar combined with standard mono?drug therapy group (42 cases), and platinum?based doublet chemotherapy group ( 90 cases) . Kaplan?Meier and Log?rank analyses were used to estimate and compare the survival rates in the groups, and Cox′s hazard regression model was used to determine the prognostic factors. Chi?square test was used to analyze the differences among different groups. Results The median progression?free survivals ( mPFS) were 50 days, 54 days, and 79 days ( P=0.042) for the standard mono?drug therapy group, endostar combined with standard mono?drug therapy group, and platinum?based doublet chemotherapy group, respectively. The differences between the mono?drug therapy group and doublet chemotherapy group were statistically significant ( P=0. 011 ) . The disease control rate ( DCR ) for each group was 26.1%, 47.6% and 46.7% (P=0.041), and the DCR were statistically significantly different between the mono?drug therapy group and doublet chemotherapy group ( P=0.016) , and between the mono?drug therapy group and endostar combined with standard mono?drug therapy group (P=0.041). The overall response rate (ORR) for each group was 2.2%, 0, and 4.4% (P>0.05 for all). Multivariate analysis showed that the period from the begining of first?line to second?line chemotherapy ( progression?free time) , base?line clinical stage, neuron specific enolase ( NSE) before second?line therapy, the cycles of second?line chemotherapy and the response to second?line therapy were independent predictors for PFS ( P<0. 005 for all) . Subgroup analysis indicated that the patients obtained more clinical benefit from doublet chemotherapy rather than mono?drug therapy, with following factors: age<60 years, paclitaxel plus cisplatin for first?line treatment, chemotherapy cycles≤4, CR, PR and SD for response, progression time within 3?6 months from the begining of first?line to second?line chemotherapy, performance status score≤1 at the begining of second?line therapy,Ⅳ stage, and mild leukopenia ( P<0.05 for all) . The patients whose progression?free survival time within 3?6 months from the begining of the first?line to second?line chemotherapy got more clinical benefit from endostar combined with standard mono?drug chemotherapy than mono?drug therapy ( P=0.006) . Conclusions The period from the begining of first?line to second?line chemotherapy, base?line TNM stage, NSE before second?line chemotherapy, the cycles of second?line chemotherapy and the response to second?line therapy were independent predictors for PFS. Platinum?based doublet chemotherapy and endostar plus standard second?line regimen can improve the efficacy in some characterized advanced NSCLC as compared with the patients by standard mono?drug therapy, wherein the platinum?based chemotherapy revealed the best efficacy.

OBJECTIVETo investigate the relationship between the stages of calcification of the third molar and age among children in Chengdu.

METHODSThe study subjects consisted of children between 2009 and 2011 at the Department of Orthodontics, West China Hospital of Stomatology, Sichuan University. Panoramic radiographs were obtained. The stages of calcification of the third molar were analyzed by Demirjian's method. The development of the third molar between the male and female was evaluated by independent samples t-test analysis. The Spearman rank correlation was applied to analyze the stages of calcification of the third molar and chronological age.

RESULTSThe development of the third molar of the male was different from that of the female at stage A of calcification of the third molar(P=0.026). The Spearman rank correlation coefficient revealed highly significant relationships between the stages of calcification of the third molar and chronological age of the three groups (male, r=0.808, P=0.000; female, r= 0.729, P=0.000; all, r=0.760, P=0.000). Furthermore, the linear regression equations were established as follows: Yall= 4.898+2.036X: Ymale=4.987+2.046X; Yfemale=4.819+2.036X.

CONCLUSIONThe stages of calcification of the third molar may be an available indicator in judging chronological age of teenager.

Adolescent ; Age Determination by Teeth ; China ; Female ; Hospitals ; Humans ; Male ; Molar, Third ; Oral Medicine ; Radiography, Panoramic ; Universities