No abstract available.
Agglutination* ; Alisma* ; Animals ; Erythrocytes* ; Mice* ; Plantago*

CBX protein(chromoboxin protein homolog)is an important member of PcG(polycombe group proteins)family protein,and PcG protein is an epigenetic regulatory complex that exists in the form of polymeric transcriptional inhibitory complexes PRCs.PcG protein can modify the target gene to transcript chromatin and plays an important role in stem cell differentiation and tumorigenesis and metastasis.The CBX protein family is an important member of the classic PRC1.Recent studies have focused on the different functions of CBX in stem cells,further demonstrating the composition and function of CBX.From more evidence,CBX plays an important role in tumorigenesis and development.This review summarizes the current advances in the study of CBX family members in tumors.

The syndrome of malignant migrating partial seizures in infancy (MMPSI) is one of the most severe epileptic encephalopathies of infancy onset.It has been firstly reported by Coppola in 1995.There have been approximately 80 cases reported worldwide by now,but there is no case reported in our country.The etiology for MMPSI is still unknown,the diagnosis is difficult,and conventional antiepileptic drugs have little efficacy.This paper reviews the possible causes,clinical characteristics,diagnosis and treatment of MMPSI.

Objective To explore the value of the combination of endoscopic sphincterotomy (EST) and laparoscopic cholecystestomy (LC) in the treatment of Cholecystolithiasis with extraheptatic bile duct stones. Methods 62 cases with cholecystolithiasis combined with extraheptatic bile duct stones were underwent LC following EST. Results Two cases were treated with common bile duct exploration after the failure of EST. 60 cases were executed sucessfully by EST and LC. No case occured serious complications. 8 patients with transient hyperamylasemia and 4 cases with intestinal bleeding after operation were cured by means of conservative therapy. Conclusion Combination of EST and LC is a safe and effective method in the treatment of cholecystolithiasis with extraheptatic bile duct stones.

Objective To investigate the cause of shallow anterior chamber after filtered operation and determine how to treat shallow anterior chamber.Methods 196 eyes of 168 cases who had suffered from glaucoma underwent trabeculectomy.The number of eyes and the extent of shallow anterior chamber were observed and treated correspondently.Results There were 39 eyes with shallow anterior chamber among 196 eyes,and the percentage was 20%.Over fluent drainage,aqueous humor leaking,choroidal detachment and malignant glaucoma were the main causes of shallow anterior chamber after trabculectomy.Careful operation good,good reconstruction and timely management were crucial to decrease the complications.Conclusion Shallow anterior chamber can not be fully preventable,but its happening can be minimized and cured effectively.It is significant to protect visual function.

Objective:To investigate the expression of Multiple resistance-associated protein-1(MRP1) and P-glycoprotein(P-pg) in oral squamous cell carcinoma(OSCC) before and after neoadjuvant chemotherapy(NACT) and its relation with tumor chemosensitivity and related clinical pathological factors.Methods:The specimens of 106 patients with OSCC before and after NACT were examined by SP immunohistochemistry.The correlation between P-pg and MRP1 expression and therapeutic effect was analyzed.Results:(1)P-pg positive expression rate in OSCC after NACT was 74.53%,which was significantly higher than thatbefore NACT(41.51%)(P﹤0.05);the effective rate of patient with P-pg negative expression before chemotherapy was 56.45%,which was significantly higher than that of patient with P-pg positive expression(34.09%)(P﹤0.05);P-pg expression levels were positively relative to the differentiation of the tumor(P﹤0.05),but not relative to clinical stages and cervical lymph node metastasis(P﹥0.05);(2)MRP1 positive expression rates in OSCC before and after NACT were 49.06%、and 57.55% respectively and the difference was not significant(P﹥0.05);the effective rates of patient with MRP1 negative expression in OSCC before chemotherapy were 50.00% and 44.23% respectively and the difference was not significant(P﹥0.05);MRP1 expression levels were not relative to clinical stages,differentiation of the tumor or cervical lymph node metastasis(P﹥0.05);Conclusion:P-pg and MRP1 have high expression in primary oral squamous cell carcinoma,but only P-pg expression levels are significantly associated with response to chemotherapy and it can be used as a predictive marker of the sensitivity to chemotherapy in patients with OSCC.

Objective:To investigate the effect of PEG-PEI/Fe3O4 Nano-magnetic fluid-TK on Apoptosis of liver cancer cells(HepG2)in vitro.Methods:Construcr ecombinant plasmid PEGFP-AFP-TK was constructed,which was delivered by nano-magnetic fuilds into AFP positive HepG2 cells and AFP negative SMMC7721 cells.The fluorescence was detected in order to evaluate the transfection rate 12h after transfection RT-PCR and Western blot were used to detect expression of HSV-TKgene at 48 h after transfection.MTT assay was used to evaluate the effect of HSV-TK on the proliferation of HepG2 cells,Flow cytometry was used to analyze the apoptosis of HepG2 cells.Results:Nano-Magnetic fluids delivered plasmid PEGFP-AFP-TK into HepG2 cells and HSV-TK gene was successfully expressed.The transfection eficacy of nano-magnetic fluids was superior than lipofectamine in HepG2 cells.RT-PCR and Western blot demonstrated that TK gene was expressed in HepG2 cells after being transfected with nano-magnetic fluids/PEGFP-AFP-TK.MTT and flow cytometry showed HSV-TK gene exerts a cell-killing effect.Conclusions:Nano-Magnetic fluids could successfully deliver PEGFP-AFP-TKinto HepG2 cells and induce expression of HSV-TK,it may become a promising gene vector for liver cancer gene therapy.AFP enhancer can specifically enhance the expression of target gene within the cell with positive AFP.

Objective:To prepare semiconductor quantum dots (QDs)-Smad4 monoclonal antibody Fluorescent probes ,and to detect nucleation shifting process of Smad4 signal protein in Rat dental papillae cells(RDPCs) stimulated by TGF-?1 with the Fluorescent probes. Methods:①QDs were chemically modified with Smad4 proteins to prepare water soluble QDs-Smad4 monoclonal antibody Fluorescent probes which were purified after the preparation. ②The properties of these probes were studied through absorption band analyses,fluorescence spectra,fluorescence spectrum analyses and confocal laser scanning fluorescence microscopy. ③The location of Smad4 proteins in RDPCs was studied with SP anti-Smad4 immunocytochemical method,QDs direct mark method,Smad4 monoclonal antibody direct mark method,and direct immunofluorescence imaging.And the properties of Quantum Dots-Smad4 monoclonal antibody Fluorescent probes in RDPCs were detected. ④After RDPCs ware cultivated by TGF-?1 for 0 hours,12 hours,and 14 hours,the dynamic nucleation shifting process of Smad4 signal protein in RDPCs was separately detected with SP anti-Smad4 immunocytochemical method and direct immunofluorescence imaging.Results:QDs and monoclonal antibody ware covalently bonded to form the fluorescent probes which could specifically and effectively recognize Smad4 proteins in RDPCs .It is to demonstrate the dynamic nucleation shifting process of Smad4 signal protein in RDPCs. These Fluorescent probes still had good properties,including broad excite spectra,narrow emission spectra,high fluorescence intensity,and photostability. Conclusion:QDs and monoclonal antibody could be covalently bonded to form the fluorescent probes with distinct optics character,special immunity recognition capability and the ability of imaging mark the protein in cells for long time,which provides the scientific evidence that QDs can visually research the movement and interaction of protein in living cell .

Objective To study the effect of the ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture on the VEGF mRNA expression of ECV-304 cell. Method Angelica sinensis and Hedysarum polybotrys’s mixture were refined by ultra-filtration technique. ECV-304 cells were cultured as model, and its proliferation was detected by MTT colorimetry. VEGF mRNA expression was observed by semi-quantitative RT-PCR. Results The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture could markly promote the growth of ECV-304 cells, there was significant difference between experimental and control group (P

Recently, we are confronted with prblems of dcelince in emplpyment rate,increase in contract breach and empleyement imbalance among different majors, ect. In this paper, we improved em-ployment work from the apects of implementation concept and methods of occupation career planning ed-ucation, entrepreneurship education, employment information system construction and quality feedback mechanism construction.