OBJECTIVETo study the chemical constituents in the stems of Kadsura oblongifolia.

METHODThe chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data.

RESULTSeven compounds named kaempferol-3-O-alpha-L-arabinofuranoside (1), kaempferol-3-O-alpha-D-arabinopyranoside (2), quercetin-3-O-alpha-D-arabinopyranoside (3), quercetin-3-O-alpha- L-arabinofuranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin (6), kaempferol (7) were isolated and identified.

CONCLUSIONCompounds 1-7 were obtained from this plant for the first time. Compounds 1-4 were isolated from Schisandraceae family for the first time.

A new dibenzocyclooctadiene lignan, renchangianin E (1) was isolated from the stems of Kadsura renchangiana. Its structure and stereochemistry were elucidated by spectroscopic methods, including 2D-NMR techniques.
Cyclooctanes ; chemistry ; Kadsura ; chemistry ; Lignans ; chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure

AIM: To study the dibenzocylooctadiene lignans from the stems of Kadsura heteroclita. METHOD: Chromatographic separations of silica gel and semi-preparative HPLC were used. All of the structures were elucidated on the basis of spectroscopic analysis, including 2D-NMR and HR-MS techniques. RESULTS: Four dibenzocylooctadiene lignans were isolated from K. heteroclita. Their structures were identified as heteroclitin R (1), heteroclitin S (2), gonisin O (3), and schisanlignone A (4). CONCLUSION Heteroclitin R (1) and heteroclitin S (2) are new natural lignans.
Kadsura ; chemistry ; Lignans ; chemistry ; isolation & purification ; Molecular Structure ; Plant Extracts ; chemistry

As a part of our search for bioactive material, the chemical investigation of the stems of Kadsura heteroclita led up to the isolation of a new lignan, heteroclitin R. The structure was elucidated on the basis of nuclear magnetic resonance spectroscopic data. Heteroclitin R was a new compound as a lignan dimer obtained from Schisandraceae family for the first time.
Dimerization ; Kadsura ; chemistry ; Lignans ; chemistry ; isolation & purification ; Molecular Structure ; Plant Extracts ; chemistry ; isolation & purification ; Plant Stems ; chemistry

The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.
Chromatography, High Pressure Liquid ; Circular Dichroism ; Cyclooctanes ; chemistry ; Kadsura ; chemistry ; Lignans ; chemistry ; Molecular Structure ; Oxygen ; Plant Extracts ; chemistry ; Stereoisomerism

To study the chemical constituents of K. oblongifolia, silica gel column chromatography, MCI and Sephadex LH-20 were used to separate the 70% acetone extract of the stems of K. oblongifolia. The structures of the isolated compounds have been established on the basis of physicochemical and NMR spectroscopic evidence as well as ESI-MS in some cases. Twenty compounds were obtained and identified as heteroclitalignan A (1), kadsulignan F (2), kadoblongifolin C (3), schizanrin F (4), heteroclitalignan C (5), kadsurarin (6), kadsulignan O (7), eburicol (8), meso-dihydroguaiaretic acid (9), kadsufolin A (10), tiegusanin M (11), heteroclitin B (12), (7'S)-parabenzlactone (13), angeloylbinankadsurin B (14), propinquain H (15), quercetin (16), kadsulignan P (17), schizanrin G (18), micrandilactone C (19) and (-)-shikimic acid (20). Compouds 1, 5, 8, 11-15, 18 and 20 were isolated from this plant for the first time. Toxicity of compounds 1-10 were evaluated with zebrafish model to observe the effect on its embryonic development and heart function. The results showed that compounds 7, 9 and 10 caused edema of zebrafish embryo and decreased the heart rate of zebrafish, which exhibited interference effect on heart development of zebrafish.
Animals ; Embryo, Nonmammalian ; drug effects ; Guaiacol ; analogs & derivatives ; toxicity ; Kadsura ; chemistry ; Lignans ; toxicity ; Plant Extracts ; toxicity ; Quercetin ; toxicity ; Triterpenes ; toxicity ; Zebrafish ; embryology

Family Schisandraceae is composed of two genera Schisandra and Kadsura, which are quite different in regard to the used part and medical function. Traditionally, the fruits of Schisandra plants were called 'Wuweizi' used as tonic for neurasthenia, antitussive, and sedative agents. The stems and roots of Kadsura plants, with names related to 'XueTeng', possess activating blood circulation and eliminating stasis and are used for the treatment of rheumatism, fractures and irregular menstruation. The dried fruit of Schisandra chinensis was recorded in Chinese Pharmacopoeia (2010 Edition) as 'Wuweizi', while the dried fruit of S. sphenanthera was recorded as an official origin of 'Nanwuweizi'. Historically, there was no evidence that the fruits of Kadsura plants were substituted as 'Wuweizi' either in ancient literatures or contemporary marketing. However, genus Kadsura is still popularly called 'Nanwuweizi' and plant K. longipedunculata is regarded as the origin of 'Nanwuweizi', thus this will cause confusion as well as misunderstanding of genus Kadsura. The authors recommended, therefore, the Chinese name of genus Kadsura should be 'Lengfantengshu' and K. longipedunculata with the name of 'Lengfanteng' in order to guarantee the drug authenticity.
Anticoagulants ; isolation & purification ; Antitussive Agents ; isolation & purification ; Drug Contamination ; Drugs, Chinese Herbal ; isolation & purification ; Fruit ; chemistry ; Kadsura ; chemistry ; Plant Roots ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; classification ; Schisandra ; classification ; Terminology as Topic

AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS. RESULTS: Two compounds were determined as (7'S,8'S,8R)-(8β,8'α)-dimethyl-4,4'-dihydroxy-5,3'-dimethoxy-5'-cyclolignan glucoside (1) and micrandiactone H (2), respectively. CONCLUSION Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide (NO) production in lipopolysaccharide-induced RAW264.7 macrophages.
Animals ; Cell Line ; Glycosides ; chemistry ; isolation & purification ; pharmacology ; Kadsura ; chemistry ; Lignin ; chemistry ; isolation & purification ; pharmacology ; Macrophages ; drug effects ; metabolism ; Magnetic Resonance Spectroscopy ; Mass Spectrometry ; Mice ; Molecular Structure ; Nitric Oxide ; metabolism ; Plant Extracts ; chemistry ; isolation & purification ; pharmacology ; Plant Roots ; chemistry

The progress in the research of the chemical constituents and their pharmacological activities of Kadsura was summarized. These chemical constituents mainly contain lignanoids and triterpnoids, much of which are characteristic ingredients of the plant and have such activities as anti-oxidation, anti-HIV, and platelet active factor antagonists.
Anti-HIV Agents ; pharmacology ; Antioxidants ; pharmacology ; Cyclooctanes ; chemistry ; isolation & purification ; Dioxoles ; chemistry ; isolation & purification ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Kadsura ; chemistry ; Lactones ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Molecular Structure ; Plants, Medicinal ; chemistry ; Platelet Activating Factor ; antagonists & inhibitors ; Triterpenes ; chemistry ; isolation & purification