Objective To study the effects of wedged insoles at different location on loads and motion of the knee joint. Methods Ten healthy female subjects participated in this experiment. Their gait patterns were assessed as six different wedged insoles and one controlled flat insole. The gait was monitored to study the kinetic changes in joint by using Motion Analysis System and force platforms. One way repeated measurement ANOVA was used to analyze the influences. Results Compared with the control group, the first peak of knee adduction moment was significant reduced by using medial forefoot wedged insole (P<0.05). The second peak of knee adduction moment was significantly reduced by both lateral forefoot wedged insole (P<0.05) and full-length lateral wedged insole (P<0.05). Conclusions The wedged insoles can effectively decrease the knee adduction moment in stance phase during walking, which will help to design an appropriate insole to reduce the knee pain due to osteoarthritis.