4.Double blind comparative study on the effect of low energy laser irradiation to rheumatoid arthritis.
Kenichi MIYAGI ; Y. OHOTANI ; S. SUZUKI ; A. AMANO ; T. AZUMA ; H. YOSHIZAWA ; A. HASHIMOTO ; Y. ISHIHARA ; K. MATSUTA
The Journal of The Japanese Society of Balneology, Climatology and Physical Medicine 1989;52(3):117-126
In order to investigate the safety and efficacy of low energy laser irradiation to the arthritis of the patients with rheumatoid arthrtis, a multi-center double blind comparative study was conducted.
Sixty eight patients with rheumatoid arthritis were randomly allocated into two groups of laser and sham irradiation. The each inflamed knee joint received 3 minut's irradiation of Ga-AI-As laser at 20mw with wave length of 830nm or 3 minut's sham irradiation, twice a week during 5 weeks. Evaluation items included pain and swelling of the knee joint, 15m walking time, ESR and morning stiffness. Routine laboratory tests including CBC, blood chemistry such as GOT, GPT, and LDH were also examined at the entry of the study and the completion of 10 times irradiation. Among various evaluation items, 15m walking time showed significant decrease in the laser group comparing with that in the sham group (P<0.01). The remaining items showed no significant differences between two groups. Laboratory data showed no remarkable changes during the study and no apparent adverse effect with irradiation was observed.
These results show some beneficial effect of low energy laser therapy to the patient with rheumatoid arthritis and also safety of irradiation.
5.What Is the Most Effective Drug Delivery System for Cisplatin during the Treatment of Hepatic Tumors with Single-Session Transcatheter Chemotherapy? A Pilot Study.
Yusuke KAWAMURA ; Kenji IKEDA ; Taito FUKUSHIMA ; Yuya SEKO ; Tasuku HARA ; Hitomi SEZAKI ; Tetsuya HOSAKA ; Norio AKUTA ; Masahiro KOBAYASHI ; Satoshi SAITOH ; Fumitaka SUZUKI ; Yoshiyuki SUZUKI ; Yasuji ARASE ; Hiromitsu KUMADA
Gut and Liver 2013;7(5):576-584
BACKGROUND/AIMS: The aim of this study was to determine the pharmacodynamics of cisplatin following three different treatment procedures for intrahepatic arterial infusion therapy for hepatocellular carcinoma (HCC). METHODS: We divided 13 HCC patients into the following three groups: group A, lone injection of cisplatin (n=3); group B, combined injection of cisplatin and lipiodol, with embolization using small gelatin cubes (GCs) (n=5); and group C, injection of suspended lipiodol with cisplatin powder, with embolization using small GCs (n=5). In each group, the free cisplatin concentration in the hepatic vein was measured at 0, 5, 10, and 30 minutes. RESULTS: The mean free cisplatin concentrations were as follows. For group A, the mean was 48.58 microg/mL at 0 minute, 7.31 microg/mL at 5 minutes, 5.70 microg/mL at 10 minutes, and 7.15 microg/mL at 30 minutes. For the same time points, for group B, the concentrations were 8.66, 4.23, 3.22, and 1.65 microg/mL, respectively, and for group C, the concentrations were 4.81, 2.61, 2.52, and 1.75 microg/mL, respectively. The mean area under the curve (AUC)0-infinity for the free cisplatin concentration was 7.80 in group A, 2.48 in group B, and 2.27 in group C. The AUC0-infinity for the free cisplatin concentration gradually decreased, from group A to group C. CONCLUSIONS: These results indicate that the combination of lipiodol and small GCs may be useful for delaying cisplatin drainage from the liver.
Carcinoma, Hepatocellular
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Cisplatin
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Drainage
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Drug Delivery Systems
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Ethiodized Oil
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Gelatin
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Hepatic Veins
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Humans
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Liver
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Pilot Projects
6.Ovipositional Behavior of Anopheles gambiae Mosquitoes
Jeffrey Agyapong ; Joseph Chabi ; Aikins Ablorde ; Worlasi D Kartey ; Joseph H. N. Osei ; Dziedzom K. de Souza ; Samuel Dadzie ; Daniel A. Boakye ; Nobuo Ohta ; Melinda P. Hadi ; Takashi Suzuki
Tropical Medicine and Health 2014;():-
Mosquito eggs laid within two hours are necessary for transgenic (injection) studies, because mosquito eggs become hard after that period. Thus, in order to have eggs available within this two-hour window, it is important for transgenic studies to understand the ovipositional behavior of Anopheles gambiae s.s.. In the present study, the ovipositional behavior of An. gambiae s.s. (Kisumu) was investigated in several different conditions: age of mosquitoes, time post blood meal to access oviposition substrate, and light conditions. Two groups of mosquitoes, 3–5 day old and 9–11 day old mosquitoes were blood-fed. For those mosquito groups, an oviposition dish was set either at 48 hours or 72 hours after the blood meal either in a light condition or in an artificial dark condition. The number of laid eggs was compared among different conditions. The 3–5 day old mosquitoes apparently produced a higher number of eggs than 9–11 day old ones, while there was no significant difference between the 2 groups. The number of laid eggs per one surviving blood-fed mosquito in the dark condition was significantly higher than that in the light condition (p = 0.03). Providing an oviposition dish at 72 hours after blood meal resulted in a significantly higher number of laid eggs per one surviving blood-fed mosquito compared to providing an oviposition dish at 48 hours after blood meal (p = 0.03). In conclusion, the optimal condition to have readily available egg supply in the present study for transgenic analysis was as follows: 3–5 days old mosquitoes with an oviposition dish placed at 72 hours after the blood meal in a dark environment.
7.Ovipositional Behavior of Anopheles gambiae Mosquitoes
Jeffrey Agyapong ; Joseph Chabi ; Aikins Ablorde ; Worlasi D. Kartey ; Joseph H.N. Osei ; Dziedzom K. de Souza ; Samuel Dadzie ; Daniel A. Boakye ; Nobuo Ohta ; Melinda P. Hadi ; Takashi Suzuki
Tropical Medicine and Health 2014;42(4):187-190
Mosquito eggs laid within two hours are necessary for transgenic (injection) studies, because mosquito eggs become hard after that period. Thus, in order to have eggs available within this two-hour window, it is important to understand the ovipositional behavior of Anopheles gambiae s.s.. In the present study, the ovipositional behavior of An. gambiae s.s. (Kisumu) was investigated in several different conditions: age of mosquitoes, time post blood meal to access oviposition substrate, and light conditions. Two groups of mosquitoes, 3–5 days old and 9–11 days old were blood-fed. For those mosquito groups, an oviposition dish was set either at 48 hours or 72 hours after the blood meal either in a light condition or in an artificial dark condition. The number of laid eggs was compared among the different conditions. The 3–5 day-old mosquitoes apparently produced a higher number of eggs than 9–11 day-old mosquitoes, while there was no significant difference between the two groups. The number of laid eggs per one surviving blood-fed mosquito in the dark condition was significantly higher than that in the light condition (p = 0.03). Providing an oviposition dish at 72 hours after blood meal resulted in a significantly higher number of laid eggs per one surviving blood-fed mosquito than at 48 hours after blood meal (p = 0.03). In conclusion, the optimal condition to have readily available egg supply for transgenic analysis was as follows: 3–5 day-old mosquitoes with an oviposition dish placed at 72 hours after the blood meal in a dark environment.
8.PHYSICAL ACTIVITY ATTENUATES THE DETRIMENTAL ASSOCIATION OF SITTING TIME WITH CARDIORESPIRATORY FITNESS IN ASIAN YOUTHS: THE ASIA-FIT STUDY
T. KIDOKORO ; K. SUZUKI ; H. NAITO ; G. BALASEKARAN ; JK. SONG ; SY. PARK, ; YM. LIOU ; D. LU ; BK. POH ; K. KIJBOONCHOO ; C. SHEN ; SS. HUI
Japanese Journal of Physical Fitness and Sports Medicine 2018;67(1):79-79
9.Micromolar sodium fluoride mediates anti-osteoclastogenesis in Porphyromonas gingivalis-induced alveolar bone loss.
Ujjal K BHAWAL ; Hye-Jin LEE ; Kazumune ARIKAWA ; Michiharu SHIMOSAKA ; Masatoshi SUZUKI ; Toshizo TOYAMA ; Takenori SATO ; Ryota KAWAMATA ; Chieko TAGUCHI ; Nobushiro HAMADA ; Ikuo NASU ; Hirohisa ARAKAWA ; Koh SHIBUTANI
International Journal of Oral Science 2015;7(4):242-249
Osteoclasts are bone-specific multinucleated cells generated by the differentiation of monocyte/macrophage lineage precursors. Regulation of osteoclast differentiation is considered an effective therapeutic approach to the treatment of bone-lytic diseases. Periodontitis is an inflammatory disease characterized by extensive bone resorption. In this study, we investigated the effects of sodium fluoride (NaF) on osteoclastogenesis induced by Porphyromonas gingivalis, an important colonizer of the oral cavity that has been implicated in periodontitis. NaF strongly inhibited the P. gingivalis-induced alveolar bone loss. That effect was accompanied by decreased levels of cathepsin K, interleukin (IL)-1β, matrix metalloproteinase 9 (MMP9), and tartrate-resistant acid phosphatase, which were up-regulated during P. gingivalis-induced osteoclastogenesis. Consistent with the in vivo anti-osteoclastogenic effect, NaF inhibited osteoclast formation caused by the differentiation factor RANKL (receptor activator of nuclear factor κB ligand) and macrophage colony-stimulating factor (M-CSF). The RANKL-stimulated induction of the transcription factor nuclear factor of activated T cells (NFAT) c1 was also abrogated by NaF. Taken together, our data demonstrate that NaF inhibits RANKL-induced osteoclastogenesis by reducing the induction of NFATc1, ultimately leading to the suppressed expression of cathepsin K and MMP9. The in vivo effect of NaF on the inhibition of P. gingivalis-induced osteoclastogenesis strengthens the potential usefulness of NaF for treating periodontal diseases.
Acid Phosphatase
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drug effects
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Alveolar Bone Loss
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microbiology
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prevention & control
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Animals
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Anti-Bacterial Agents
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therapeutic use
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Anti-Inflammatory Agents
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therapeutic use
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Bacteroidaceae Infections
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microbiology
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prevention & control
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Bone Density Conservation Agents
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therapeutic use
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Cathepsin K
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drug effects
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Interleukin-1beta
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drug effects
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Interleukin-6
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analysis
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Interleukin-8
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drug effects
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Isoenzymes
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drug effects
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Macrophage Colony-Stimulating Factor
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drug effects
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Male
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Matrix Metalloproteinase 9
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drug effects
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Osteoclasts
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drug effects
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Periodontitis
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microbiology
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prevention & control
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Porphyromonas gingivalis
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drug effects
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RANK Ligand
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drug effects
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Rats
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Rats, Sprague-Dawley
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Sodium Fluoride
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therapeutic use
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Tartrate-Resistant Acid Phosphatase
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Transcription Factors
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drug effects
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X-Ray Microtomography
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methods
10.Integration of publicly available case-based data for real-time coronavirus disease 2019 risk assessment, Japan
Kota Ninomiya ; Mariko Kanamori ; Naomi Ikeda ; Kazuaki Jindai ; Yura K Ko ; Kanako Otani ; Yuki Furuse ; Hiroki Akaba ; Reiko Miyahara ; Mayuko Saito ; Motoi Suzuki ; Hitoshi Oshitani
Western Pacific Surveillance and Response 2022;13(1):43-48
In response to the outbreak of coronavirus disease 2019 (COVID-19) in Japan, a national COVID-19 cluster taskforce (comprising governmental and nongovernmental experts) was established to support the country’s Ministry of Health, Labour and Welfare in conducting daily risk assessment. The assessment was carried out using established infectious disease surveillance systems; however, in the initial stages of the pandemic these were not sufficient for real-time risk assessment owing to limited accessibility, delay in data entry and inadequate case information. Also, local governments were publishing anonymized data on confirmed COVID-19 cases on their official websites as daily press releases. We developed a unique database for nationwide real-time risk assessment that included these case lists from local government websites and integrated all case data into a standardized format. The database was updated daily and checked systematically to ensure comprehensiveness and quality. Between 15 January 2020 and 15 June 2021, 776 459 cases were logged in the database, allowing for analysis of real-time risk from the pandemic. This semi-automated database was used in daily risk assessments, and to evaluate and update control measures to prevent community transmission of COVID-19 in Japan. The data were reported almost every week to the Japanese Government Advisory Panel on COVID-19 for public health responses.